Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula; Rhodopirellula baltica

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7UJF6|Q7UJF6_RHOBA Transport permease protein OS=Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1) OX=243090 GN=RB11929 PE=3 SV=1
MM1 pKa = 7.22EE2 pKa = 4.19VVEE5 pKa = 5.1IMNHH9 pKa = 6.23HH10 pKa = 7.37DD11 pKa = 5.35SDD13 pKa = 4.64DD14 pKa = 4.77LDD16 pKa = 4.61DD17 pKa = 5.03ASQTRR22 pKa = 11.84RR23 pKa = 11.84DD24 pKa = 3.37KK25 pKa = 11.1RR26 pKa = 11.84IGEE29 pKa = 4.21EE30 pKa = 4.24SPSDD34 pKa = 5.19LVPEE38 pKa = 4.47WQRR41 pKa = 11.84LLNDD45 pKa = 4.44DD46 pKa = 5.57PLPQDD51 pKa = 5.27DD52 pKa = 4.84NWDD55 pKa = 3.97SPDD58 pKa = 3.5PTATDD63 pKa = 3.3AALDD67 pKa = 3.74GTYY70 pKa = 10.59VCDD73 pKa = 3.5NCGEE77 pKa = 4.37EE78 pKa = 4.31IVIPLDD84 pKa = 2.98IAAGRR89 pKa = 11.84DD90 pKa = 3.34QQYY93 pKa = 10.01VEE95 pKa = 5.0DD96 pKa = 5.29CPVCCSPSVIHH107 pKa = 6.36VHH109 pKa = 6.98FDD111 pKa = 3.58DD112 pKa = 5.54SDD114 pKa = 3.99HH115 pKa = 6.93ADD117 pKa = 2.79VWAEE121 pKa = 3.96AEE123 pKa = 3.91QDD125 pKa = 3.26RR126 pKa = 11.84YY127 pKa = 11.03

Molecular weight: 14.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7UJR5|Q7UJR5_RHOBA Uncharacterized protein OS=Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1) OX=243090 GN=RB11114 PE=4 SV=1
MM1 pKa = 7.58LWNKK5 pKa = 9.38ARR7 pKa = 11.84RR8 pKa = 11.84PTTPARR14 pKa = 11.84PNAVRR19 pKa = 11.84PITPPGTTWPTTPPSQPAALARR41 pKa = 11.84PPHH44 pKa = 6.19RR45 pKa = 11.84TNSWRR50 pKa = 11.84IRR52 pKa = 11.84LRR54 pKa = 11.84NPNPTRR60 pKa = 11.84QRR62 pKa = 11.84GTTLNPGQSPTTPRR76 pKa = 11.84GG77 pKa = 3.22

Molecular weight: 8.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7271

0

7271

2290149

35

8173

315.0

34.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.271 ± 0.034

1.267 ± 0.015

6.237 ± 0.036

6.014 ± 0.031

3.699 ± 0.016

7.488 ± 0.044

2.305 ± 0.022

4.95 ± 0.018

3.408 ± 0.03

9.335 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.431 ± 0.017

3.372 ± 0.027

5.332 ± 0.031

4.03 ± 0.025

6.935 ± 0.048

7.375 ± 0.032

5.856 ± 0.047

7.053 ± 0.032

1.544 ± 0.015

2.096 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski