Mycobacterium sp. ACS1612

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; unclassified Mycobacterium

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6361 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A1YRU0|A0A1A1YRU0_9MYCO Peroxidase OS=Mycobacterium sp. ACS1612 OX=1834117 GN=A5724_17530 PE=4 SV=1
MM1 pKa = 7.16NRR3 pKa = 11.84PYY5 pKa = 10.01TAYY8 pKa = 10.29SPYY11 pKa = 10.87SLTPTEE17 pKa = 5.32RR18 pKa = 11.84IPSQWDD24 pKa = 3.41DD25 pKa = 3.38GSEE28 pKa = 4.21TVDD31 pKa = 4.33LDD33 pKa = 3.59RR34 pKa = 11.84DD35 pKa = 3.72RR36 pKa = 11.84YY37 pKa = 11.13DD38 pKa = 4.51DD39 pKa = 5.53DD40 pKa = 4.72EE41 pKa = 7.01GPDD44 pKa = 3.38EE45 pKa = 4.58YY46 pKa = 11.38EE47 pKa = 5.32DD48 pKa = 3.37IEE50 pKa = 4.65EE51 pKa = 4.34YY52 pKa = 10.92YY53 pKa = 10.93EE54 pKa = 4.65PIDD57 pKa = 5.46RR58 pKa = 11.84RR59 pKa = 11.84WIWIAAVAGVILLVAVICTVVILGGGDD86 pKa = 3.27SGSVSTTVAPPVTTSQPAATTSAPPVTTSAAPPPAATLPPEE127 pKa = 4.33TVTTVTPTPTAVATPTDD144 pKa = 4.03TPAQAAPAPAAAPNTITYY162 pKa = 9.8RR163 pKa = 11.84VTGQRR168 pKa = 11.84SLLDD172 pKa = 4.02LVTVIYY178 pKa = 10.26TDD180 pKa = 3.26SQGALQTDD188 pKa = 4.16VNVALPWAKK197 pKa = 9.15TVTLDD202 pKa = 3.73PGVTLGSVTATSVTGQLNCSITDD225 pKa = 3.57AAGTLVAVQNNNTMIANCTKK245 pKa = 10.86

Molecular weight: 25.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A1Z580|A0A1A1Z580_9MYCO Protein-export membrane protein SecF OS=Mycobacterium sp. ACS1612 OX=1834117 GN=secF PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSNRR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.77GRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 8.65LTAA47 pKa = 3.95

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6361

0

6361

2047305

36

4356

321.9

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.941 ± 0.044

0.821 ± 0.009

6.373 ± 0.027

5.201 ± 0.028

3.102 ± 0.018

8.701 ± 0.031

2.209 ± 0.014

4.334 ± 0.021

2.356 ± 0.019

9.767 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.096 ± 0.012

2.309 ± 0.016

5.737 ± 0.028

3.055 ± 0.017

7.104 ± 0.03

5.384 ± 0.017

6.025 ± 0.024

8.79 ± 0.029

1.505 ± 0.014

2.189 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski