Amino acid dipepetide frequency for Mycobacterium sp. ACS1612

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.74AlaAla: 19.74 ± 0.147
1.003AlaCys: 1.003 ± 0.023
8.642AlaAsp: 8.642 ± 0.076
7.65AlaGlu: 7.65 ± 0.075
3.594AlaPhe: 3.594 ± 0.045
11.154AlaGly: 11.154 ± 0.097
2.56AlaHis: 2.56 ± 0.037
5.371AlaIle: 5.371 ± 0.051
3.316AlaLys: 3.316 ± 0.048
12.745AlaLeu: 12.745 ± 0.099
3.025AlaMet: 3.025 ± 0.042
2.757AlaAsn: 2.757 ± 0.041
6.12AlaPro: 6.12 ± 0.073
4.018AlaGln: 4.018 ± 0.05
8.263AlaArg: 8.263 ± 0.074
6.2AlaSer: 6.2 ± 0.06
7.228AlaThr: 7.228 ± 0.068
11.946AlaVal: 11.946 ± 0.094
1.64AlaTrp: 1.64 ± 0.03
2.445AlaTyr: 2.445 ± 0.034
0.0AlaXaa: 0.0 ± 0.0
Cys
1.08CysAla: 1.08 ± 0.024
0.083CysCys: 0.083 ± 0.007
0.555CysAsp: 0.555 ± 0.018
0.421CysGlu: 0.421 ± 0.013
0.225CysPhe: 0.225 ± 0.011
0.956CysGly: 0.956 ± 0.024
0.189CysHis: 0.189 ± 0.01
0.274CysIle: 0.274 ± 0.011
0.134CysLys: 0.134 ± 0.008
0.69CysLeu: 0.69 ± 0.019
0.131CysMet: 0.131 ± 0.008
0.166CysAsn: 0.166 ± 0.01
0.473CysPro: 0.473 ± 0.014
0.221CysGln: 0.221 ± 0.01
0.596CysArg: 0.596 ± 0.018
0.48CysSer: 0.48 ± 0.014
0.494CysThr: 0.494 ± 0.015
0.706CysVal: 0.706 ± 0.018
0.146CysTrp: 0.146 ± 0.008
0.191CysTyr: 0.191 ± 0.009
0.0CysXaa: 0.0 ± 0.0
Asp
7.947AspAla: 7.947 ± 0.082
0.462AspCys: 0.462 ± 0.015
4.498AspAsp: 4.498 ± 0.055
4.194AspGlu: 4.194 ± 0.044
1.902AspPhe: 1.902 ± 0.031
5.969AspGly: 5.969 ± 0.058
1.404AspHis: 1.404 ± 0.027
2.667AspIle: 2.667 ± 0.041
1.447AspLys: 1.447 ± 0.028
5.915AspLeu: 5.915 ± 0.053
1.098AspMet: 1.098 ± 0.023
1.36AspAsn: 1.36 ± 0.027
4.296AspPro: 4.296 ± 0.05
1.795AspGln: 1.795 ± 0.031
4.632AspArg: 4.632 ± 0.049
2.861AspSer: 2.861 ± 0.049
3.166AspThr: 3.166 ± 0.04
5.645AspVal: 5.645 ± 0.061
0.99AspTrp: 0.99 ± 0.022
1.481AspTyr: 1.481 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
6.171GluAla: 6.171 ± 0.07
0.39GluCys: 0.39 ± 0.014
2.485GluAsp: 2.485 ± 0.039
2.459GluGlu: 2.459 ± 0.039
1.864GluPhe: 1.864 ± 0.031
3.294GluGly: 3.294 ± 0.037
1.538GluHis: 1.538 ± 0.029
2.624GluIle: 2.624 ± 0.035
1.444GluLys: 1.444 ± 0.031
6.425GluLeu: 6.425 ± 0.066
1.094GluMet: 1.094 ± 0.025
1.156GluAsn: 1.156 ± 0.027
3.015GluPro: 3.015 ± 0.042
2.283GluGln: 2.283 ± 0.035
4.245GluArg: 4.245 ± 0.051
2.68GluSer: 2.68 ± 0.038
2.647GluThr: 2.647 ± 0.037
4.358GluVal: 4.358 ± 0.047
0.728GluTrp: 0.728 ± 0.019
1.113GluTyr: 1.113 ± 0.021
0.0GluXaa: 0.0 ± 0.0
Phe
4.087PheAla: 4.087 ± 0.05
0.308PheCys: 0.308 ± 0.01
2.526PheAsp: 2.526 ± 0.033
1.622PheGlu: 1.622 ± 0.027
1.027PhePhe: 1.027 ± 0.025
3.516PheGly: 3.516 ± 0.049
0.638PheHis: 0.638 ± 0.017
1.174PheIle: 1.174 ± 0.028
0.596PheLys: 0.596 ± 0.02
2.554PheLeu: 2.554 ± 0.04
0.529PheMet: 0.529 ± 0.017
0.818PheAsn: 0.818 ± 0.02
1.401PhePro: 1.401 ± 0.027
0.687PheGln: 0.687 ± 0.02
1.739PheArg: 1.739 ± 0.029
1.751PheSer: 1.751 ± 0.028
2.146PheThr: 2.146 ± 0.034
2.714PheVal: 2.714 ± 0.042
0.468PheTrp: 0.468 ± 0.015
0.722PheTyr: 0.722 ± 0.02
0.0PheXaa: 0.0 ± 0.0
Gly
9.885GlyAla: 9.885 ± 0.082
0.812GlyCys: 0.812 ± 0.02
5.042GlyAsp: 5.042 ± 0.06
4.259GlyGlu: 4.259 ± 0.044
3.06GlyPhe: 3.06 ± 0.043
7.682GlyGly: 7.682 ± 0.1
2.014GlyHis: 2.014 ± 0.03
4.164GlyIle: 4.164 ± 0.043
2.396GlyLys: 2.396 ± 0.038
8.518GlyLeu: 8.518 ± 0.072
2.142GlyMet: 2.142 ± 0.033
1.977GlyAsn: 1.977 ± 0.038
4.403GlyPro: 4.403 ± 0.057
2.84GlyGln: 2.84 ± 0.038
6.07GlyArg: 6.07 ± 0.057
4.925GlySer: 4.925 ± 0.059
5.086GlyThr: 5.086 ± 0.055
7.694GlyVal: 7.694 ± 0.062
1.634GlyTrp: 1.634 ± 0.03
2.41GlyTyr: 2.41 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.424HisAla: 2.424 ± 0.036
0.214HisCys: 0.214 ± 0.01
1.389HisAsp: 1.389 ± 0.026
1.067HisGlu: 1.067 ± 0.024
0.726HisPhe: 0.726 ± 0.019
2.109HisGly: 2.109 ± 0.031
0.665HisHis: 0.665 ± 0.017
0.866HisIle: 0.866 ± 0.019
0.402HisLys: 0.402 ± 0.014
2.106HisLeu: 2.106 ± 0.035
0.378HisMet: 0.378 ± 0.014
0.515HisAsn: 0.515 ± 0.015
1.649HisPro: 1.649 ± 0.027
0.684HisGln: 0.684 ± 0.02
1.852HisArg: 1.852 ± 0.029
1.115HisSer: 1.115 ± 0.022
1.226HisThr: 1.226 ± 0.024
1.754HisVal: 1.754 ± 0.025
0.4HisTrp: 0.4 ± 0.015
0.546HisTyr: 0.546 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.579IleAla: 6.579 ± 0.06
0.423IleCys: 0.423 ± 0.014
3.444IleAsp: 3.444 ± 0.04
2.675IleGlu: 2.675 ± 0.039
1.099IlePhe: 1.099 ± 0.027
4.656IleGly: 4.656 ± 0.053
0.803IleHis: 0.803 ± 0.019
1.489IleIle: 1.489 ± 0.029
0.962IleLys: 0.962 ± 0.024
3.008IleLeu: 3.008 ± 0.044
0.62IleMet: 0.62 ± 0.019
1.173IleAsn: 1.173 ± 0.028
2.379IlePro: 2.379 ± 0.034
0.997IleGln: 0.997 ± 0.025
2.674IleArg: 2.674 ± 0.039
2.321IleSer: 2.321 ± 0.033
2.797IleThr: 2.797 ± 0.036
3.91IleVal: 3.91 ± 0.047
0.493IleTrp: 0.493 ± 0.018
0.839IleTyr: 0.839 ± 0.02
0.0IleXaa: 0.0 ± 0.0
Lys
2.983LysAla: 2.983 ± 0.046
0.128LysCys: 0.128 ± 0.008
1.276LysAsp: 1.276 ± 0.03
1.038LysGlu: 1.038 ± 0.026
0.695LysPhe: 0.695 ± 0.021
1.659LysGly: 1.659 ± 0.03
0.544LysHis: 0.544 ± 0.016
1.05LysIle: 1.05 ± 0.024
0.739LysLys: 0.739 ± 0.03
2.381LysLeu: 2.381 ± 0.032
0.538LysMet: 0.538 ± 0.018
0.534LysAsn: 0.534 ± 0.019
1.641LysPro: 1.641 ± 0.033
0.818LysGln: 0.818 ± 0.018
1.731LysArg: 1.731 ± 0.028
1.366LysSer: 1.366 ± 0.027
1.459LysThr: 1.459 ± 0.026
2.092LysVal: 2.092 ± 0.035
0.367LysTrp: 0.367 ± 0.015
0.518LysTyr: 0.518 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
13.8LeuAla: 13.8 ± 0.091
0.826LeuCys: 0.826 ± 0.017
6.384LeuAsp: 6.384 ± 0.072
4.375LeuGlu: 4.375 ± 0.053
2.765LeuPhe: 2.765 ± 0.04
8.564LeuGly: 8.564 ± 0.07
2.053LeuHis: 2.053 ± 0.032
4.256LeuIle: 4.256 ± 0.056
1.99LeuLys: 1.99 ± 0.028
9.701LeuLeu: 9.701 ± 0.098
1.821LeuMet: 1.821 ± 0.03
2.195LeuAsn: 2.195 ± 0.031
5.666LeuPro: 5.666 ± 0.058
2.691LeuGln: 2.691 ± 0.039
7.397LeuArg: 7.397 ± 0.068
5.631LeuSer: 5.631 ± 0.051
6.33LeuThr: 6.33 ± 0.058
8.294LeuVal: 8.294 ± 0.074
1.227LeuTrp: 1.227 ± 0.027
1.707LeuTyr: 1.707 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.72MetAla: 2.72 ± 0.038
0.19MetCys: 0.19 ± 0.008
0.894MetAsp: 0.894 ± 0.019
0.735MetGlu: 0.735 ± 0.018
0.66MetPhe: 0.66 ± 0.017
1.489MetGly: 1.489 ± 0.025
0.438MetHis: 0.438 ± 0.014
0.905MetIle: 0.905 ± 0.022
0.509MetLys: 0.509 ± 0.018
2.068MetLeu: 2.068 ± 0.032
0.462MetMet: 0.462 ± 0.015
0.531MetAsn: 0.531 ± 0.015
1.289MetPro: 1.289 ± 0.024
0.595MetGln: 0.595 ± 0.018
1.644MetArg: 1.644 ± 0.026
1.529MetSer: 1.529 ± 0.027
1.932MetThr: 1.932 ± 0.032
1.728MetVal: 1.728 ± 0.028
0.284MetTrp: 0.284 ± 0.011
0.361MetTyr: 0.361 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.813AsnAla: 2.813 ± 0.043
0.211AsnCys: 0.211 ± 0.011
1.297AsnAsp: 1.297 ± 0.028
1.052AsnGlu: 1.052 ± 0.022
0.68AsnPhe: 0.68 ± 0.022
2.204AsnGly: 2.204 ± 0.038
0.497AsnHis: 0.497 ± 0.015
1.03AsnIle: 1.03 ± 0.024
0.529AsnLys: 0.529 ± 0.016
2.182AsnLeu: 2.182 ± 0.033
0.453AsnMet: 0.453 ± 0.014
0.615AsnAsn: 0.615 ± 0.019
1.814AsnPro: 1.814 ± 0.031
0.655AsnGln: 0.655 ± 0.02
1.613AsnArg: 1.613 ± 0.028
1.183AsnSer: 1.183 ± 0.026
1.344AsnThr: 1.344 ± 0.027
1.903AsnVal: 1.903 ± 0.031
0.414AsnTrp: 0.414 ± 0.015
0.599AsnTyr: 0.599 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
6.933ProAla: 6.933 ± 0.074
0.308ProCys: 0.308 ± 0.014
4.422ProAsp: 4.422 ± 0.05
3.475ProGlu: 3.475 ± 0.049
1.679ProPhe: 1.679 ± 0.028
5.319ProGly: 5.319 ± 0.055
1.25ProHis: 1.25 ± 0.028
2.182ProIle: 2.182 ± 0.034
1.441ProLys: 1.441 ± 0.032
4.956ProLeu: 4.956 ± 0.046
1.213ProMet: 1.213 ± 0.025
1.394ProAsn: 1.394 ± 0.03
3.851ProPro: 3.851 ± 0.086
1.867ProGln: 1.867 ± 0.033
3.265ProArg: 3.265 ± 0.043
3.098ProSer: 3.098 ± 0.044
3.54ProThr: 3.54 ± 0.045
5.05ProVal: 5.05 ± 0.05
0.88ProTrp: 0.88 ± 0.022
1.248ProTyr: 1.248 ± 0.021
0.0ProXaa: 0.0 ± 0.0
Gln
3.712GlnAla: 3.712 ± 0.045
0.232GlnCys: 0.232 ± 0.011
1.249GlnAsp: 1.249 ± 0.022
1.165GlnGlu: 1.165 ± 0.024
1.044GlnPhe: 1.044 ± 0.025
1.973GlnGly: 1.973 ± 0.032
0.748GlnHis: 0.748 ± 0.017
1.566GlnIle: 1.566 ± 0.027
0.684GlnLys: 0.684 ± 0.017
3.604GlnLeu: 3.604 ± 0.047
0.714GlnMet: 0.714 ± 0.018
0.666GlnAsn: 0.666 ± 0.019
2.008GlnPro: 2.008 ± 0.03
1.401GlnGln: 1.401 ± 0.029
2.813GlnArg: 2.813 ± 0.037
1.555GlnSer: 1.555 ± 0.03
1.696GlnThr: 1.696 ± 0.027
2.452GlnVal: 2.452 ± 0.041
0.598GlnTrp: 0.598 ± 0.018
0.672GlnTyr: 0.672 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
8.081ArgAla: 8.081 ± 0.079
0.604ArgCys: 0.604 ± 0.021
4.343ArgAsp: 4.343 ± 0.049
3.732ArgGlu: 3.732 ± 0.045
2.429ArgPhe: 2.429 ± 0.033
5.061ArgGly: 5.061 ± 0.057
1.733ArgHis: 1.733 ± 0.031
3.492ArgIle: 3.492 ± 0.047
1.643ArgLys: 1.643 ± 0.03
7.354ArgLeu: 7.354 ± 0.07
1.768ArgMet: 1.768 ± 0.032
1.696ArgAsn: 1.696 ± 0.032
3.7ArgPro: 3.7 ± 0.045
2.353ArgGln: 2.353 ± 0.04
6.41ArgArg: 6.41 ± 0.074
3.84ArgSer: 3.84 ± 0.046
4.078ArgThr: 4.078 ± 0.048
5.465ArgVal: 5.465 ± 0.064
1.314ArgTrp: 1.314 ± 0.025
1.942ArgTyr: 1.942 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
6.926SerAla: 6.926 ± 0.058
0.418SerCys: 0.418 ± 0.014
3.318SerAsp: 3.318 ± 0.043
2.638SerGlu: 2.638 ± 0.042
1.649SerPhe: 1.649 ± 0.026
5.421SerGly: 5.421 ± 0.053
1.094SerHis: 1.094 ± 0.023
2.253SerIle: 2.253 ± 0.033
1.236SerLys: 1.236 ± 0.027
4.8SerLeu: 4.8 ± 0.051
1.381SerMet: 1.381 ± 0.024
1.137SerAsn: 1.137 ± 0.023
3.087SerPro: 3.087 ± 0.039
1.584SerGln: 1.584 ± 0.03
3.664SerArg: 3.664 ± 0.045
3.1SerSer: 3.1 ± 0.044
3.445SerThr: 3.445 ± 0.042
4.597SerVal: 4.597 ± 0.051
0.877SerTrp: 0.877 ± 0.022
1.214SerTyr: 1.214 ± 0.022
0.0SerXaa: 0.0 ± 0.0
Thr
7.846ThrAla: 7.846 ± 0.064
0.419ThrCys: 0.419 ± 0.015
3.776ThrAsp: 3.776 ± 0.041
3.115ThrGlu: 3.115 ± 0.043
1.894ThrPhe: 1.894 ± 0.03
5.751ThrGly: 5.751 ± 0.054
1.179ThrHis: 1.179 ± 0.026
2.482ThrIle: 2.482 ± 0.038
1.41ThrLys: 1.41 ± 0.031
5.602ThrLeu: 5.602 ± 0.057
1.211ThrMet: 1.211 ± 0.025
1.333ThrAsn: 1.333 ± 0.028
3.795ThrPro: 3.795 ± 0.048
1.553ThrGln: 1.553 ± 0.03
3.541ThrArg: 3.541 ± 0.039
3.311ThrSer: 3.311 ± 0.04
3.968ThrThr: 3.968 ± 0.066
5.907ThrVal: 5.907 ± 0.064
0.799ThrTrp: 0.799 ± 0.019
1.356ThrTyr: 1.356 ± 0.029
0.0ThrXaa: 0.0 ± 0.0
Val
11.642ValAla: 11.642 ± 0.085
0.774ValCys: 0.774 ± 0.021
5.96ValAsp: 5.96 ± 0.059
4.624ValGlu: 4.624 ± 0.046
2.686ValPhe: 2.686 ± 0.043
7.185ValGly: 7.185 ± 0.065
1.848ValHis: 1.848 ± 0.031
4.108ValIle: 4.108 ± 0.049
1.9ValLys: 1.9 ± 0.035
8.929ValLeu: 8.929 ± 0.08
1.72ValMet: 1.72 ± 0.034
2.135ValAsn: 2.135 ± 0.037
4.661ValPro: 4.661 ± 0.051
2.159ValGln: 2.159 ± 0.032
5.78ValArg: 5.78 ± 0.06
4.805ValSer: 4.805 ± 0.053
5.495ValThr: 5.495 ± 0.054
8.758ValVal: 8.758 ± 0.082
1.095ValTrp: 1.095 ± 0.025
1.634ValTyr: 1.634 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.558TrpAla: 1.558 ± 0.031
0.167TrpCys: 0.167 ± 0.009
0.792TrpAsp: 0.792 ± 0.02
0.626TrpGlu: 0.626 ± 0.016
0.526TrpPhe: 0.526 ± 0.017
1.012TrpGly: 1.012 ± 0.023
0.399TrpHis: 0.399 ± 0.015
0.694TrpIle: 0.694 ± 0.019
0.34TrpLys: 0.34 ± 0.013
1.765TrpLeu: 1.765 ± 0.027
0.336TrpMet: 0.336 ± 0.012
0.405TrpAsn: 0.405 ± 0.014
0.854TrpPro: 0.854 ± 0.02
0.641TrpGln: 0.641 ± 0.019
1.262TrpArg: 1.262 ± 0.029
0.939TrpSer: 0.939 ± 0.023
0.918TrpThr: 0.918 ± 0.02
1.147TrpVal: 1.147 ± 0.023
0.323TrpTrp: 0.323 ± 0.012
0.352TrpTyr: 0.352 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.487TyrAla: 2.487 ± 0.035
0.243TyrCys: 0.243 ± 0.011
1.438TyrAsp: 1.438 ± 0.029
1.091TyrGlu: 1.091 ± 0.024
0.819TyrPhe: 0.819 ± 0.019
2.039TyrGly: 2.039 ± 0.039
0.492TyrHis: 0.492 ± 0.015
0.691TyrIle: 0.691 ± 0.021
0.438TyrLys: 0.438 ± 0.015
2.372TyrLeu: 2.372 ± 0.036
0.331TyrMet: 0.331 ± 0.013
0.524TyrAsn: 0.524 ± 0.016
1.268TyrPro: 1.268 ± 0.028
0.711TyrGln: 0.711 ± 0.02
1.845TyrArg: 1.845 ± 0.034
1.152TyrSer: 1.152 ± 0.025
1.255TyrThr: 1.255 ± 0.028
1.779TyrVal: 1.779 ± 0.029
0.377TyrTrp: 0.377 ± 0.012
0.537TyrTyr: 0.537 ± 0.018
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6361 proteins (2047305 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski