Panax notoginseng virus A
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U4DRM0|A0A0U4DRM0_9VIRU RNA-directed RNA polymerase OS=Panax notoginseng virus A OX=1777016 PE=3 SV=1
MM1 pKa = 8.04 DD2 pKa = 3.91 SFVRR6 pKa = 11.84 QFFPDD11 pKa = 4.24 FNSPGVDD18 pKa = 4.0 FNFDD22 pKa = 3.6 SDD24 pKa = 4.69 LKK26 pKa = 7.94 THH28 pKa = 6.63 TYY30 pKa = 8.2 MGCGLRR36 pKa = 11.84 VYY38 pKa = 10.42 DD39 pKa = 4.36 GHH41 pKa = 8.14 IDD43 pKa = 3.7 QNRR46 pKa = 11.84 HH47 pKa = 3.72 FHH49 pKa = 6.26 SFQPMTPLGHH59 pKa = 6.54 YY60 pKa = 10.12 DD61 pKa = 3.51 CEE63 pKa = 4.44 FTGGSTSLDD72 pKa = 3.31 GLTKK76 pKa = 10.67 NYY78 pKa = 7.4 LTPEE82 pKa = 4.08 GTVDD86 pKa = 3.67 LTALADD92 pKa = 3.9 TLKK95 pKa = 10.99 GSAGLQTALLSAHH108 pKa = 6.1 VSHH111 pKa = 6.65 MLHH114 pKa = 6.67 WSWADD119 pKa = 3.08 NHH121 pKa = 6.02 VALLFNMFRR130 pKa = 11.84 YY131 pKa = 10.57 VMIEE135 pKa = 4.39 RR136 pKa = 11.84 IRR138 pKa = 11.84 EE139 pKa = 3.93 AAGSLVGKK147 pKa = 10.32 LGLYY151 pKa = 10.14 EE152 pKa = 5.19 DD153 pKa = 3.88 GHH155 pKa = 8.41 IGIDD159 pKa = 3.35 MDD161 pKa = 4.12 QFWKK165 pKa = 10.6 EE166 pKa = 4.19 SYY168 pKa = 10.55 PEE170 pKa = 4.55 DD171 pKa = 4.51 GGDD174 pKa = 3.54 RR175 pKa = 11.84 CWPGGGDD182 pKa = 3.57 DD183 pKa = 5.7 LSYY186 pKa = 10.91 PDD188 pKa = 4.96 YY189 pKa = 11.07 IRR191 pKa = 11.84 LTATTPSTGDD201 pKa = 2.83 WALDD205 pKa = 3.48 LRR207 pKa = 11.84 GMSARR212 pKa = 11.84 DD213 pKa = 3.39 ARR215 pKa = 11.84 FVLLMLGSWKK225 pKa = 9.98 RR226 pKa = 11.84 RR227 pKa = 11.84 SRR229 pKa = 11.84 LRR231 pKa = 11.84 LDD233 pKa = 3.53 CSTPRR238 pKa = 11.84 LCDD241 pKa = 3.21 NVLYY245 pKa = 10.27 RR246 pKa = 11.84 SSVEE250 pKa = 3.85 VTNITDD256 pKa = 4.42 WISDD260 pKa = 3.67 KK261 pKa = 11.21 DD262 pKa = 3.66 KK263 pKa = 10.81 QAPPRR268 pKa = 11.84 TLSSSEE274 pKa = 3.19 AWRR277 pKa = 11.84 ALKK280 pKa = 10.58 LYY282 pKa = 7.81 VTQNRR287 pKa = 11.84 LYY289 pKa = 10.65 DD290 pKa = 3.75 HH291 pKa = 7.15 FSATLYY297 pKa = 9.95 TISTMMYY304 pKa = 8.84 QFVPATAEE312 pKa = 3.87 ATNWLSLHH320 pKa = 4.97 WTVNIPTFRR329 pKa = 11.84 AVRR332 pKa = 11.84 GRR334 pKa = 11.84 YY335 pKa = 8.4 EE336 pKa = 3.74 FLNEE340 pKa = 3.86 GEE342 pKa = 4.42 AALLSHH348 pKa = 7.25 RR349 pKa = 11.84 SLNEE353 pKa = 3.09 WGYY356 pKa = 10.98 INGKK360 pKa = 8.13 LEE362 pKa = 4.17 KK363 pKa = 9.75 VNLMALVFSQAYY375 pKa = 6.63 HH376 pKa = 6.12 TGLAVRR382 pKa = 11.84 GVRR385 pKa = 11.84 KK386 pKa = 9.88 GLEE389 pKa = 3.79 LHH391 pKa = 6.81 PNDD394 pKa = 3.89 VFASEE399 pKa = 3.82 IDD401 pKa = 4.06 FYY403 pKa = 11.37 TSANFISAAASEE415 pKa = 4.55 ATRR418 pKa = 11.84 TDD420 pKa = 3.11 TPLPGMVGIHH430 pKa = 6.25 FVTDD434 pKa = 4.09 DD435 pKa = 4.56 DD436 pKa = 5.02 FDD438 pKa = 3.79 MYY440 pKa = 11.58 NDD442 pKa = 3.52 DD443 pKa = 4.06 RR444 pKa = 11.84 KK445 pKa = 10.93 VEE447 pKa = 4.06 TRR449 pKa = 11.84 EE450 pKa = 3.93 NDD452 pKa = 3.77 DD453 pKa = 4.06 EE454 pKa = 5.68 LLDD457 pKa = 5.2 DD458 pKa = 3.95 YY459 pKa = 10.29 HH460 pKa = 7.36 TSISKK465 pKa = 10.57 VGLKK469 pKa = 10.26 KK470 pKa = 10.29 GTTILVDD477 pKa = 3.17 IADD480 pKa = 3.79 TRR482 pKa = 11.84 LEE484 pKa = 4.46 KK485 pKa = 10.35 VTLDD489 pKa = 3.38 QVKK492 pKa = 10.49 KK493 pKa = 10.54 KK494 pKa = 10.3 IRR496 pKa = 11.84 TLTIGANAKK505 pKa = 10.47 LKK507 pKa = 10.46 DD508 pKa = 3.88 DD509 pKa = 4.52 PEE511 pKa = 5.8 FEE513 pKa = 4.33 LTEE516 pKa = 4.18 EE517 pKa = 4.26 EE518 pKa = 4.42 SMAMEE523 pKa = 4.35 GKK525 pKa = 9.74 MYY527 pKa = 10.8 VSLSADD533 pKa = 3.31 EE534 pKa = 5.07 DD535 pKa = 3.93 DD536 pKa = 3.86 VRR538 pKa = 11.84 EE539 pKa = 4.13 DD540 pKa = 3.18 RR541 pKa = 11.84 VFVHH545 pKa = 5.87 MPWYY549 pKa = 9.19 TFAGVPTMILPLSPFPYY566 pKa = 8.96 NTPFNMKK573 pKa = 8.87 GTISPEE579 pKa = 3.65 LGKK582 pKa = 9.49 MDD584 pKa = 3.14 RR585 pKa = 11.84 RR586 pKa = 11.84 GFRR589 pKa = 11.84 LMSRR593 pKa = 11.84 NAWEE597 pKa = 3.9 IANLLRR603 pKa = 11.84 LCGYY607 pKa = 8.28 DD608 pKa = 2.96 ARR610 pKa = 11.84 MRR612 pKa = 11.84 DD613 pKa = 3.36 GGEE616 pKa = 3.49 IAGAGTYY623 pKa = 7.78 FAPNDD628 pKa = 3.92 ANMVWPVMQVPDD640 pKa = 4.16 AQDD643 pKa = 3.59 DD644 pKa = 4.11 DD645 pKa = 5.4 VILTGQIDD653 pKa = 3.9 RR654 pKa = 11.84 EE655 pKa = 4.38 VQFIDD660 pKa = 4.61 LPPVYY665 pKa = 10.71 NRR667 pKa = 11.84 FFTGKK672 pKa = 9.77 KK673 pKa = 8.26 IEE675 pKa = 4.07 YY676 pKa = 9.79 SITVLKK682 pKa = 10.67 RR683 pKa = 11.84 GTATSFTDD691 pKa = 3.06 NRR693 pKa = 11.84 KK694 pKa = 9.76 DD695 pKa = 3.4 VAEE698 pKa = 4.08 WGGPVTLTRR707 pKa = 11.84 EE708 pKa = 4.01 VTVTYY713 pKa = 8.66 EE714 pKa = 4.03 VPEE717 pKa = 4.25 SVSRR721 pKa = 11.84 LRR723 pKa = 11.84 AYY725 pKa = 9.86 ISRR728 pKa = 11.84 NEE730 pKa = 3.91 SGFRR734 pKa = 11.84 FVDD737 pKa = 3.4 NAPAGVIPMLEE748 pKa = 4.0 RR749 pKa = 11.84 EE750 pKa = 3.97 IDD752 pKa = 3.64 VQGVVEE758 pKa = 4.4 DD759 pKa = 3.82
Molecular weight: 85.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.951
IPC2_protein 4.952
IPC_protein 4.94
Toseland 4.863
ProMoST 5.067
Dawson 4.94
Bjellqvist 5.08
Wikipedia 4.838
Rodwell 4.838
Grimsley 4.774
Solomon 4.94
Lehninger 4.902
Nozaki 5.067
DTASelect 5.27
Thurlkill 4.863
EMBOSS 4.876
Sillero 5.118
Patrickios 4.037
IPC_peptide 4.952
IPC2_peptide 5.118
IPC2.peptide.svr19 5.076
Protein with the highest isoelectric point:
>tr|A0A0U4DRM0|A0A0U4DRM0_9VIRU RNA-directed RNA polymerase OS=Panax notoginseng virus A OX=1777016 PE=3 SV=1
MM1 pKa = 7.45 SKK3 pKa = 10.48 VPLTRR8 pKa = 11.84 NNIPAWCNIRR18 pKa = 11.84 HH19 pKa = 6.09 NNLSACSRR27 pKa = 11.84 EE28 pKa = 3.75 KK29 pKa = 10.48 AGYY32 pKa = 8.53 VLGHH36 pKa = 5.78 ITYY39 pKa = 9.98 DD40 pKa = 3.06 IKK42 pKa = 11.4 SSVGGFGAHH51 pKa = 5.04 RR52 pKa = 11.84 VGNNSVRR59 pKa = 11.84 SVAVKK64 pKa = 10.94 CEE66 pKa = 4.04 DD67 pKa = 2.92 FWLLYY72 pKa = 10.95 VKK74 pKa = 7.58 TTHH77 pKa = 7.47 DD78 pKa = 3.71 ATLLPPMIRR87 pKa = 11.84 RR88 pKa = 11.84 ALSVAYY94 pKa = 10.0 SQVDD98 pKa = 4.01 GYY100 pKa = 11.4 DD101 pKa = 3.38 YY102 pKa = 11.51 SDD104 pKa = 3.16 WQLFRR109 pKa = 11.84 YY110 pKa = 9.09 LRR112 pKa = 11.84 RR113 pKa = 11.84 EE114 pKa = 3.93 FKK116 pKa = 10.55 VDD118 pKa = 2.8 RR119 pKa = 11.84 KK120 pKa = 9.62 MITHH124 pKa = 6.75 ARR126 pKa = 11.84 VGTGPRR132 pKa = 11.84 PEE134 pKa = 4.23 EE135 pKa = 3.95 FPRR138 pKa = 11.84 ATVTGEE144 pKa = 3.63 HH145 pKa = 6.15 HH146 pKa = 4.79 THH148 pKa = 6.31 FRR150 pKa = 11.84 PEE152 pKa = 4.18 EE153 pKa = 3.74 VWEE156 pKa = 4.07 VSKK159 pKa = 10.81 KK160 pKa = 10.16 YY161 pKa = 9.66 RR162 pKa = 11.84 AKK164 pKa = 10.17 LRR166 pKa = 11.84 AMSVVYY172 pKa = 10.96 NNLKK176 pKa = 10.25 KK177 pKa = 10.32 IEE179 pKa = 4.3 GVTEE183 pKa = 4.08 AVVSTMFLYY192 pKa = 10.34 ILFARR197 pKa = 11.84 PQTAYY202 pKa = 11.14 LFACSAKK209 pKa = 8.58 IWSVRR214 pKa = 11.84 DD215 pKa = 3.35 INGLSDD221 pKa = 3.32 ILKK224 pKa = 9.45 TISTPLKK231 pKa = 10.05 SVQNNDD237 pKa = 3.34 LCDD240 pKa = 3.51 VTQLFEE246 pKa = 4.32 LQCLVNRR253 pKa = 11.84 GIGKK257 pKa = 9.47 VDD259 pKa = 3.02 WRR261 pKa = 11.84 KK262 pKa = 9.57 EE263 pKa = 3.58 RR264 pKa = 11.84 ANRR267 pKa = 11.84 EE268 pKa = 3.93 KK269 pKa = 10.75 PNVVIVDD276 pKa = 3.7 EE277 pKa = 4.51 KK278 pKa = 11.02 KK279 pKa = 10.72 VYY281 pKa = 10.22 HH282 pKa = 6.23 EE283 pKa = 4.18 AMNLFRR289 pKa = 11.84 MGISHH294 pKa = 7.1 GYY296 pKa = 9.6 KK297 pKa = 9.17 YY298 pKa = 11.44 ARR300 pKa = 11.84 MDD302 pKa = 3.43 LNKK305 pKa = 9.92 YY306 pKa = 9.09 VKK308 pKa = 10.38 SRR310 pKa = 11.84 WEE312 pKa = 3.92 WVPSGSVHH320 pKa = 5.65 SQYY323 pKa = 11.25 EE324 pKa = 3.76 EE325 pKa = 4.0 DD326 pKa = 3.5 EE327 pKa = 4.58 PYY329 pKa = 10.15 IKK331 pKa = 10.1 KK332 pKa = 10.88 SNMHH336 pKa = 5.15 RR337 pKa = 11.84 TKK339 pKa = 10.58 FVTVNMLSRR348 pKa = 11.84 AHH350 pKa = 6.44 IRR352 pKa = 11.84 RR353 pKa = 11.84 IFARR357 pKa = 11.84 KK358 pKa = 9.67 KK359 pKa = 10.06 EE360 pKa = 3.84 IHH362 pKa = 6.0 AWASVKK368 pKa = 10.46 YY369 pKa = 8.97 EE370 pKa = 3.86 WAKK373 pKa = 10.25 QRR375 pKa = 11.84 AIYY378 pKa = 10.09 GVDD381 pKa = 3.43 LTSSIITNFAMFRR394 pKa = 11.84 CEE396 pKa = 3.74 EE397 pKa = 4.21 VFKK400 pKa = 11.14 HH401 pKa = 5.86 RR402 pKa = 11.84 FPVGEE407 pKa = 3.82 EE408 pKa = 3.53 AAASRR413 pKa = 11.84 VHH415 pKa = 6.42 KK416 pKa = 9.57 RR417 pKa = 11.84 LKK419 pKa = 10.36 MMLDD423 pKa = 3.72 GNEE426 pKa = 4.16 SFCYY430 pKa = 10.74 DD431 pKa = 3.41 FDD433 pKa = 5.14 DD434 pKa = 5.86 FNAQHH439 pKa = 6.12 STGSMYY445 pKa = 11.1 AVLQAYY451 pKa = 8.75 MDD453 pKa = 3.72 VFRR456 pKa = 11.84 GSMSRR461 pKa = 11.84 DD462 pKa = 3.16 QVSAMEE468 pKa = 4.1 WVRR471 pKa = 11.84 DD472 pKa = 3.78 SILSVKK478 pKa = 8.79 VHH480 pKa = 5.0 NHH482 pKa = 4.61 EE483 pKa = 4.17 EE484 pKa = 4.26 GRR486 pKa = 11.84 EE487 pKa = 3.85 EE488 pKa = 4.53 FYY490 pKa = 10.15 KK491 pKa = 10.72 TDD493 pKa = 3.38 GTLLSGWRR501 pKa = 11.84 LTTFMNTALNYY512 pKa = 9.79 IYY514 pKa = 10.46 FKK516 pKa = 10.88 LAGAFDD522 pKa = 3.84 VTGVRR527 pKa = 11.84 DD528 pKa = 3.88 SVHH531 pKa = 6.62 NGDD534 pKa = 4.63 DD535 pKa = 3.45 VLVAIRR541 pKa = 11.84 DD542 pKa = 3.53 IRR544 pKa = 11.84 SACSIHH550 pKa = 6.8 GAMARR555 pKa = 11.84 INARR559 pKa = 11.84 AQATKK564 pKa = 10.8 CNVFSVGEE572 pKa = 3.91 FLRR575 pKa = 11.84 VEE577 pKa = 4.18 HH578 pKa = 6.68 KK579 pKa = 9.07 VTRR582 pKa = 11.84 EE583 pKa = 3.68 EE584 pKa = 4.2 GLGAQYY590 pKa = 9.05 LTRR593 pKa = 11.84 AAATLAHH600 pKa = 6.4 SRR602 pKa = 11.84 IEE604 pKa = 4.27 SQEE607 pKa = 3.57 PTRR610 pKa = 11.84 LVDD613 pKa = 3.96 SIKK616 pKa = 11.09 GMVTRR621 pKa = 11.84 CEE623 pKa = 4.16 EE624 pKa = 3.43 ITARR628 pKa = 11.84 SFGMEE633 pKa = 3.73 TVAYY637 pKa = 8.82 EE638 pKa = 4.22 LLTHH642 pKa = 6.1 SVRR645 pKa = 11.84 RR646 pKa = 11.84 LCGIFGVQAEE656 pKa = 4.69 DD657 pKa = 4.18 GYY659 pKa = 11.67 KK660 pKa = 9.82 VLKK663 pKa = 10.54 GHH665 pKa = 5.79 TLVGGAMEE673 pKa = 4.64 TFDD676 pKa = 5.56 GSIKK680 pKa = 10.24 YY681 pKa = 10.04 KK682 pKa = 10.14 IRR684 pKa = 11.84 EE685 pKa = 3.84 EE686 pKa = 4.02 VLIGKK691 pKa = 7.08 TVITADD697 pKa = 3.39 NRR699 pKa = 11.84 HH700 pKa = 6.13 EE701 pKa = 4.1 YY702 pKa = 9.24 ATLKK706 pKa = 11.22 DD707 pKa = 4.07 MMPGILDD714 pKa = 3.44 YY715 pKa = 11.73 AEE717 pKa = 4.57 ILNNQYY723 pKa = 11.18 GDD725 pKa = 4.39 YY726 pKa = 10.9 IPIDD730 pKa = 3.98 NIKK733 pKa = 10.54 KK734 pKa = 9.09 KK735 pKa = 10.33 IIYY738 pKa = 7.45 ATHH741 pKa = 6.38 RR742 pKa = 11.84 QLEE745 pKa = 4.55 VTRR748 pKa = 11.84 GTRR751 pKa = 11.84 LIVTDD756 pKa = 3.64 VQHH759 pKa = 7.05 DD760 pKa = 3.75 DD761 pKa = 3.02 KK762 pKa = 11.52 YY763 pKa = 11.08 KK764 pKa = 9.83 YY765 pKa = 10.49 GRR767 pKa = 11.84 ALFRR771 pKa = 11.84 MYY773 pKa = 10.41 KK774 pKa = 10.1 GLITIPYY781 pKa = 7.8 VEE783 pKa = 4.53 KK784 pKa = 10.9 ARR786 pKa = 11.84 FLGIPPIAMLSHH798 pKa = 7.13 SSMKK802 pKa = 10.26 FVTKK806 pKa = 10.64 LISSVKK812 pKa = 10.58 DD813 pKa = 2.91 VDD815 pKa = 3.66 YY816 pKa = 10.72 TLRR819 pKa = 11.84 TLLL822 pKa = 4.17
Molecular weight: 94.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.77
IPC2_protein 8.756
IPC_protein 8.639
Toseland 9.209
ProMoST 9.107
Dawson 9.56
Bjellqvist 9.355
Wikipedia 9.75
Rodwell 9.736
Grimsley 9.648
Solomon 9.589
Lehninger 9.531
Nozaki 9.414
DTASelect 9.297
Thurlkill 9.399
EMBOSS 9.677
Sillero 9.531
Patrickios 4.66
IPC_peptide 9.575
IPC2_peptide 8.053
IPC2.peptide.svr19 7.736
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1581
759
822
790.5
90.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.021 ± 0.219
1.139 ± 0.252
6.831 ± 1.352
5.882 ± 0.157
4.364 ± 0.275
6.452 ± 0.481
2.973 ± 0.436
5.06 ± 0.708
5.313 ± 1.082
8.223 ± 0.725
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.352 ± 0.053
3.922 ± 0.022
3.416 ± 0.771
2.087 ± 0.08
7.211 ± 0.452
6.388 ± 0.047
6.705 ± 0.584
7.653 ± 0.677
1.708 ± 0.195
4.301 ± 0.348
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here