Sodiomyces alkalinus F11
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9397 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3N2PPY4|A0A3N2PPY4_9PEZI Chitinase OS=Sodiomyces alkalinus F11 OX=1314773 GN=SODALDRAFT_281833 PE=4 SV=1
MM1 pKa = 7.15 TPKK4 pKa = 10.7 SPVDD8 pKa = 3.92 EE9 pKa = 4.33 YY10 pKa = 11.88 AMGSDD15 pKa = 4.32 EE16 pKa = 4.5 EE17 pKa = 4.95 EE18 pKa = 4.19 EE19 pKa = 4.36 MANLAPGIADD29 pKa = 3.49 ICTEE33 pKa = 4.16 APPLSVALDD42 pKa = 3.51 MDD44 pKa = 3.96 EE45 pKa = 4.62 EE46 pKa = 4.49 SDD48 pKa = 3.64 YY49 pKa = 11.85 DD50 pKa = 3.67 ALFDD54 pKa = 3.99 HH55 pKa = 6.38 THH57 pKa = 5.9 QYY59 pKa = 11.78 ASLKK63 pKa = 9.02 TDD65 pKa = 3.38 STIHH69 pKa = 5.74 SQHH72 pKa = 5.75 KK73 pKa = 10.34 HH74 pKa = 5.24 IDD76 pKa = 3.76 LLDD79 pKa = 4.05 EE80 pKa = 5.54 DD81 pKa = 5.22 IDD83 pKa = 3.74 WTDD86 pKa = 2.96 VSRR89 pKa = 11.84 VTEE92 pKa = 4.26 CEE94 pKa = 4.02 APDD97 pKa = 3.91 GSVPSASS104 pKa = 3.22
Molecular weight: 11.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.592
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.719
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.91
DTASelect 4.126
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.923
Patrickios 1.888
IPC_peptide 3.783
IPC2_peptide 3.897
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A3N2PYJ6|A0A3N2PYJ6_9PEZI Uncharacterized protein OS=Sodiomyces alkalinus F11 OX=1314773 GN=SODALDRAFT_159256 PE=4 SV=1
MM1 pKa = 7.28 QFRR4 pKa = 11.84 PPPVITTLFATPPPGSSQRR23 pKa = 11.84 QRR25 pKa = 11.84 RR26 pKa = 11.84 SVARR30 pKa = 11.84 NHH32 pKa = 6.5 PIARR36 pKa = 11.84 ISRR39 pKa = 11.84 IPPVPAPPPQPMM51 pKa = 3.67
Molecular weight: 5.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9396
1
9397
4378554
49
5151
466.0
51.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.108 ± 0.025
1.231 ± 0.01
5.837 ± 0.018
6.096 ± 0.024
3.533 ± 0.016
7.159 ± 0.024
2.579 ± 0.013
4.233 ± 0.016
4.328 ± 0.022
8.764 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.089 ± 0.009
3.322 ± 0.013
6.703 ± 0.029
3.981 ± 0.019
6.928 ± 0.023
8.168 ± 0.03
5.851 ± 0.015
6.17 ± 0.02
1.403 ± 0.009
2.515 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here