Slackia heliotrinireducens (strain ATCC 29202 / DSM 20476 / NCTC 11029 / RHS 1) (Peptococcus heliotrinreducens)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Coriobacteriia; Eggerthellales; Eggerthellaceae; Slackia; Slackia heliotrinireducens

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7N1K1|C7N1K1_SLAHD tRNA N6-adenosine threonylcarbamoyltransferase OS=Slackia heliotrinireducens (strain ATCC 29202 / DSM 20476 / NCTC 11029 / RHS 1) OX=471855 GN=tsaD PE=3 SV=1
MM1 pKa = 7.59FCPNCGTQLPDD12 pKa = 3.44GSAFCGNCGNRR23 pKa = 11.84LNAAPNPAPAPGYY36 pKa = 9.84PPVAQPAGSFDD47 pKa = 3.14IKK49 pKa = 10.74KK50 pKa = 9.56FLPIIIGVAAAVLLLALFLIIRR72 pKa = 11.84PFGGGLSGEE81 pKa = 4.25NVGKK85 pKa = 10.42IMTMEE90 pKa = 4.18PEE92 pKa = 4.77KK93 pKa = 10.48IAKK96 pKa = 9.58QLDD99 pKa = 3.36KK100 pKa = 10.81MEE102 pKa = 4.32SVKK105 pKa = 10.43VDD107 pKa = 3.29GQRR110 pKa = 11.84LYY112 pKa = 11.23VSSEE116 pKa = 3.84EE117 pKa = 4.05FGDD120 pKa = 4.32LIKK123 pKa = 11.17DD124 pKa = 3.56ADD126 pKa = 4.32DD127 pKa = 4.22GLSKK131 pKa = 10.87KK132 pKa = 9.37EE133 pKa = 3.46AAAADD138 pKa = 4.31GEE140 pKa = 4.55WAFAVNPDD148 pKa = 4.2ARR150 pKa = 11.84DD151 pKa = 3.67LEE153 pKa = 5.85DD154 pKa = 5.44GDD156 pKa = 5.23DD157 pKa = 4.46LGSVSCFRR165 pKa = 11.84VYY167 pKa = 11.47ADD169 pKa = 4.61TDD171 pKa = 3.65DD172 pKa = 4.86LNCNKK177 pKa = 9.82IVDD180 pKa = 3.99YY181 pKa = 10.92AKK183 pKa = 11.31DD184 pKa = 3.21MGLDD188 pKa = 3.17VGTYY192 pKa = 9.49ACYY195 pKa = 10.62VGDD198 pKa = 4.07GGDD201 pKa = 3.54YY202 pKa = 11.18GYY204 pKa = 9.21LTAYY208 pKa = 10.21NDD210 pKa = 4.15DD211 pKa = 3.58YY212 pKa = 11.59SINLNVSIDD221 pKa = 3.44SHH223 pKa = 5.89ATVASATVYY232 pKa = 10.65TDD234 pKa = 3.96DD235 pKa = 6.29GYY237 pKa = 11.43DD238 pKa = 3.44LKK240 pKa = 11.15DD241 pKa = 3.7AQDD244 pKa = 3.52EE245 pKa = 4.7VEE247 pKa = 4.87NYY249 pKa = 10.86ADD251 pKa = 3.75NLEE254 pKa = 4.54DD255 pKa = 4.61EE256 pKa = 4.65MDD258 pKa = 3.51EE259 pKa = 4.1FDD261 pKa = 4.54YY262 pKa = 11.73EE263 pKa = 4.29EE264 pKa = 4.08IQTNVKK270 pKa = 10.15

Molecular weight: 29.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7N4A7|C7N4A7_SLAHD DMSO reductase iron-sulfur subunit OS=Slackia heliotrinireducens (strain ATCC 29202 / DSM 20476 / NCTC 11029 / RHS 1) OX=471855 GN=Shel_06830 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.31QPNKK9 pKa = 7.52RR10 pKa = 11.84HH11 pKa = 5.83RR12 pKa = 11.84AKK14 pKa = 9.65THH16 pKa = 5.05GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.76GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2750

0

2750

898832

31

3920

326.8

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.798 ± 0.067

1.65 ± 0.024

6.522 ± 0.04

6.882 ± 0.048

3.822 ± 0.026

7.985 ± 0.041

1.862 ± 0.022

5.308 ± 0.044

4.046 ± 0.034

8.689 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.937 ± 0.023

3.268 ± 0.032

4.224 ± 0.028

3.025 ± 0.027

5.411 ± 0.058

5.691 ± 0.036

5.535 ± 0.076

7.908 ± 0.043

1.173 ± 0.022

3.263 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski