Burkholderia phage KS14

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Kisquattuordecimvirus; Burkholderia virus KS14

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5FFJ6|E5FFJ6_9CAUD Gp43 OS=Burkholderia phage KS14 OX=910475 GN=43 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84VAAVVLIFAALEE14 pKa = 4.05IACASAWGVIADD26 pKa = 5.03ASDD29 pKa = 3.18TCQIDD34 pKa = 3.51DD35 pKa = 4.08EE36 pKa = 5.02KK37 pKa = 11.18ICVEE41 pKa = 4.2SIGWLEE47 pKa = 3.81RR48 pKa = 3.58

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5FFF8|E5FFF8_9CAUD Gp5 OS=Burkholderia phage KS14 OX=910475 GN=5 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84TMTSRR7 pKa = 11.84TSPAKK12 pKa = 9.86RR13 pKa = 11.84VPIPLSAEE21 pKa = 4.01QIDD24 pKa = 3.87NLQRR28 pKa = 11.84LAKK31 pKa = 9.89QEE33 pKa = 4.01QRR35 pKa = 11.84SEE37 pKa = 3.87AQMARR42 pKa = 11.84IIYY45 pKa = 9.85LVGLEE50 pKa = 4.11QYY52 pKa = 10.04SSKK55 pKa = 10.62VKK57 pKa = 10.46RR58 pKa = 11.84RR59 pKa = 3.4

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

9937

48

923

225.8

24.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.038 ± 0.663

0.765 ± 0.141

6.219 ± 0.299

5.676 ± 0.265

3.18 ± 0.221

7.668 ± 0.467

1.982 ± 0.232

4.871 ± 0.136

4.478 ± 0.297

8.876 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.207

3.009 ± 0.169

4.287 ± 0.246

3.432 ± 0.205

7.135 ± 0.425

5.515 ± 0.215

6.229 ± 0.302

6.34 ± 0.216

1.338 ± 0.143

2.405 ± 0.201
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski