Amino acid dipepetide frequency for Burkholderia phage KS14

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.82AlaAla: 18.82 ± 2.262
1.409AlaCys: 1.409 ± 0.367
8.857AlaAsp: 8.857 ± 1.078
9.259AlaGlu: 9.259 ± 0.89
3.925AlaPhe: 3.925 ± 0.681
12.681AlaGly: 12.681 ± 1.296
2.919AlaHis: 2.919 ± 0.666
6.844AlaIle: 6.844 ± 0.727
5.737AlaLys: 5.737 ± 1.169
13.99AlaLeu: 13.99 ± 1.149
3.724AlaMet: 3.724 ± 0.628
3.824AlaAsn: 3.824 ± 0.634
5.837AlaPro: 5.837 ± 1.07
5.233AlaGln: 5.233 ± 0.885
9.259AlaArg: 9.259 ± 1.289
7.448AlaSer: 7.448 ± 0.693
6.743AlaThr: 6.743 ± 1.49
7.246AlaVal: 7.246 ± 0.882
2.818AlaTrp: 2.818 ± 0.491
3.824AlaTyr: 3.824 ± 0.591
0.0AlaXaa: 0.0 ± 0.0
Cys
1.208CysAla: 1.208 ± 0.658
0.101CysCys: 0.101 ± 0.102
0.503CysAsp: 0.503 ± 0.206
0.805CysGlu: 0.805 ± 0.29
0.403CysPhe: 0.403 ± 0.196
0.503CysGly: 0.503 ± 0.219
0.0CysHis: 0.0 ± 0.0
0.302CysIle: 0.302 ± 0.211
0.302CysLys: 0.302 ± 0.214
0.604CysLeu: 0.604 ± 0.304
0.302CysMet: 0.302 ± 0.157
0.101CysAsn: 0.101 ± 0.115
0.201CysPro: 0.201 ± 0.126
0.503CysGln: 0.503 ± 0.306
0.302CysArg: 0.302 ± 0.163
0.503CysSer: 0.503 ± 0.198
0.403CysThr: 0.403 ± 0.286
0.503CysVal: 0.503 ± 0.234
0.0CysTrp: 0.0 ± 0.0
0.101CysTyr: 0.101 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
8.756AspAla: 8.756 ± 1.1
0.503AspCys: 0.503 ± 0.236
4.831AspAsp: 4.831 ± 0.669
3.824AspGlu: 3.824 ± 0.569
2.717AspPhe: 2.717 ± 0.512
4.126AspGly: 4.126 ± 0.841
1.208AspHis: 1.208 ± 0.371
2.717AspIle: 2.717 ± 0.635
2.717AspLys: 2.717 ± 0.679
5.737AspLeu: 5.737 ± 0.735
1.912AspMet: 1.912 ± 0.677
1.51AspAsn: 1.51 ± 0.441
3.12AspPro: 3.12 ± 0.477
1.711AspGln: 1.711 ± 0.43
3.623AspArg: 3.623 ± 0.621
2.919AspSer: 2.919 ± 0.47
3.925AspThr: 3.925 ± 0.607
4.026AspVal: 4.026 ± 0.649
0.705AspTrp: 0.705 ± 0.237
1.61AspTyr: 1.61 ± 0.451
0.0AspXaa: 0.0 ± 0.0
Glu
7.045GluAla: 7.045 ± 0.847
0.604GluCys: 0.604 ± 0.313
1.308GluAsp: 1.308 ± 0.324
1.409GluGlu: 1.409 ± 0.28
2.114GluPhe: 2.114 ± 0.564
3.523GluGly: 3.523 ± 0.515
1.006GluHis: 1.006 ± 0.261
3.019GluIle: 3.019 ± 0.563
3.019GluLys: 3.019 ± 0.649
7.146GluLeu: 7.146 ± 0.912
1.61GluMet: 1.61 ± 0.357
2.013GluAsn: 2.013 ± 0.492
2.315GluPro: 2.315 ± 0.536
2.415GluGln: 2.415 ± 0.542
3.824GluArg: 3.824 ± 0.605
3.724GluSer: 3.724 ± 0.765
4.126GluThr: 4.126 ± 0.699
3.321GluVal: 3.321 ± 0.634
1.409GluTrp: 1.409 ± 0.31
1.812GluTyr: 1.812 ± 0.46
0.0GluXaa: 0.0 ± 0.0
Phe
4.932PheAla: 4.932 ± 0.724
0.0PheCys: 0.0 ± 0.0
2.717PheAsp: 2.717 ± 0.422
2.114PheGlu: 2.114 ± 0.387
0.705PhePhe: 0.705 ± 0.226
2.717PheGly: 2.717 ± 0.647
0.403PheHis: 0.403 ± 0.211
1.711PheIle: 1.711 ± 0.416
1.51PheLys: 1.51 ± 0.457
1.812PheLeu: 1.812 ± 0.401
0.805PheMet: 0.805 ± 0.258
0.906PheAsn: 0.906 ± 0.263
1.409PhePro: 1.409 ± 0.509
0.805PheGln: 0.805 ± 0.351
2.617PheArg: 2.617 ± 0.501
2.013PheSer: 2.013 ± 0.405
2.415PheThr: 2.415 ± 0.495
0.805PheVal: 0.805 ± 0.387
0.604PheTrp: 0.604 ± 0.247
0.805PheTyr: 0.805 ± 0.337
0.0PheXaa: 0.0 ± 0.0
Gly
9.762GlyAla: 9.762 ± 1.499
0.302GlyCys: 0.302 ± 0.154
4.932GlyAsp: 4.932 ± 0.585
5.334GlyGlu: 5.334 ± 0.801
2.516GlyPhe: 2.516 ± 0.448
5.636GlyGly: 5.636 ± 0.931
0.705GlyHis: 0.705 ± 0.315
4.328GlyIle: 4.328 ± 0.771
4.026GlyLys: 4.026 ± 0.674
6.643GlyLeu: 6.643 ± 0.836
1.912GlyMet: 1.912 ± 0.41
2.415GlyAsn: 2.415 ± 0.547
2.415GlyPro: 2.415 ± 0.449
1.409GlyGln: 1.409 ± 0.438
5.837GlyArg: 5.837 ± 1.124
4.126GlySer: 4.126 ± 0.566
4.227GlyThr: 4.227 ± 0.738
5.837GlyVal: 5.837 ± 0.929
1.711GlyTrp: 1.711 ± 0.358
2.617GlyTyr: 2.617 ± 0.587
0.0GlyXaa: 0.0 ± 0.0
His
2.315HisAla: 2.315 ± 0.488
0.101HisCys: 0.101 ± 0.091
1.409HisAsp: 1.409 ± 0.382
1.912HisGlu: 1.912 ± 0.438
0.201HisPhe: 0.201 ± 0.128
2.114HisGly: 2.114 ± 0.478
0.302HisHis: 0.302 ± 0.201
1.208HisIle: 1.208 ± 0.401
0.805HisLys: 0.805 ± 0.25
2.315HisLeu: 2.315 ± 0.519
0.503HisMet: 0.503 ± 0.207
0.503HisAsn: 0.503 ± 0.209
0.403HisPro: 0.403 ± 0.248
0.604HisGln: 0.604 ± 0.239
1.308HisArg: 1.308 ± 0.475
0.705HisSer: 0.705 ± 0.235
0.906HisThr: 0.906 ± 0.368
1.409HisVal: 1.409 ± 0.409
0.302HisTrp: 0.302 ± 0.194
0.503HisTyr: 0.503 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
8.253IleAla: 8.253 ± 0.923
0.201IleCys: 0.201 ± 0.118
5.435IleAsp: 5.435 ± 0.912
5.032IleGlu: 5.032 ± 0.702
0.705IlePhe: 0.705 ± 0.287
5.435IleGly: 5.435 ± 0.611
0.906IleHis: 0.906 ± 0.285
0.604IleIle: 0.604 ± 0.23
1.812IleLys: 1.812 ± 0.347
2.717IleLeu: 2.717 ± 0.446
0.906IleMet: 0.906 ± 0.29
1.711IleAsn: 1.711 ± 0.399
1.812IlePro: 1.812 ± 0.489
1.308IleGln: 1.308 ± 0.379
1.812IleArg: 1.812 ± 0.435
2.214IleSer: 2.214 ± 0.454
4.126IleThr: 4.126 ± 0.824
2.617IleVal: 2.617 ± 0.584
0.302IleTrp: 0.302 ± 0.169
0.805IleTyr: 0.805 ± 0.271
0.0IleXaa: 0.0 ± 0.0
Lys
6.24LysAla: 6.24 ± 0.808
0.201LysCys: 0.201 ± 0.12
2.013LysAsp: 2.013 ± 0.447
2.818LysGlu: 2.818 ± 0.611
0.906LysPhe: 0.906 ± 0.317
4.126LysGly: 4.126 ± 0.683
1.61LysHis: 1.61 ± 0.555
1.409LysIle: 1.409 ± 0.319
2.114LysLys: 2.114 ± 0.313
3.925LysLeu: 3.925 ± 0.653
0.906LysMet: 0.906 ± 0.432
0.805LysAsn: 0.805 ± 0.311
2.214LysPro: 2.214 ± 0.527
2.114LysGln: 2.114 ± 0.57
4.63LysArg: 4.63 ± 0.662
2.516LysSer: 2.516 ± 0.409
2.617LysThr: 2.617 ± 0.5
2.013LysVal: 2.013 ± 0.474
0.403LysTrp: 0.403 ± 0.174
1.208LysTyr: 1.208 ± 0.371
0.0LysXaa: 0.0 ± 0.0
Leu
14.09LeuAla: 14.09 ± 1.481
1.006LeuCys: 1.006 ± 0.376
5.636LeuAsp: 5.636 ± 0.856
2.919LeuGlu: 2.919 ± 0.516
2.214LeuPhe: 2.214 ± 0.482
6.441LeuGly: 6.441 ± 1.534
1.711LeuHis: 1.711 ± 0.444
4.026LeuIle: 4.026 ± 0.754
3.925LeuLys: 3.925 ± 0.539
6.039LeuLeu: 6.039 ± 0.788
2.114LeuMet: 2.114 ± 0.476
3.321LeuAsn: 3.321 ± 0.541
4.227LeuPro: 4.227 ± 0.554
3.422LeuGln: 3.422 ± 0.434
7.045LeuArg: 7.045 ± 1.081
5.636LeuSer: 5.636 ± 0.743
5.737LeuThr: 5.737 ± 0.724
6.844LeuVal: 6.844 ± 0.726
0.503LeuTrp: 0.503 ± 0.228
1.912LeuTyr: 1.912 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
3.321MetAla: 3.321 ± 0.53
0.201MetCys: 0.201 ± 0.134
1.208MetAsp: 1.208 ± 0.311
0.705MetGlu: 0.705 ± 0.268
0.805MetPhe: 0.805 ± 0.227
1.006MetGly: 1.006 ± 0.343
0.604MetHis: 0.604 ± 0.207
1.308MetIle: 1.308 ± 0.391
1.308MetLys: 1.308 ± 0.398
2.717MetLeu: 2.717 ± 0.552
0.906MetMet: 0.906 ± 0.317
1.208MetAsn: 1.208 ± 0.273
1.006MetPro: 1.006 ± 0.383
1.107MetGln: 1.107 ± 0.327
3.019MetArg: 3.019 ± 0.581
1.51MetSer: 1.51 ± 0.388
2.114MetThr: 2.114 ± 0.431
1.006MetVal: 1.006 ± 0.304
0.101MetTrp: 0.101 ± 0.093
0.403MetTyr: 0.403 ± 0.174
0.0MetXaa: 0.0 ± 0.0
Asn
4.328AsnAla: 4.328 ± 0.599
0.101AsnCys: 0.101 ± 0.107
2.214AsnAsp: 2.214 ± 0.475
1.711AsnGlu: 1.711 ± 0.364
0.805AsnPhe: 0.805 ± 0.361
4.227AsnGly: 4.227 ± 0.715
0.604AsnHis: 0.604 ± 0.233
1.409AsnIle: 1.409 ± 0.271
1.107AsnLys: 1.107 ± 0.363
2.617AsnLeu: 2.617 ± 0.507
1.006AsnMet: 1.006 ± 0.278
0.503AsnAsn: 0.503 ± 0.221
1.61AsnPro: 1.61 ± 0.4
0.705AsnGln: 0.705 ± 0.208
1.308AsnArg: 1.308 ± 0.334
1.51AsnSer: 1.51 ± 0.342
1.107AsnThr: 1.107 ± 0.359
2.013AsnVal: 2.013 ± 0.354
0.101AsnTrp: 0.101 ± 0.097
1.107AsnTyr: 1.107 ± 0.386
0.0AsnXaa: 0.0 ± 0.0
Pro
8.857ProAla: 8.857 ± 1.048
0.201ProCys: 0.201 ± 0.141
3.422ProAsp: 3.422 ± 0.531
2.214ProGlu: 2.214 ± 0.442
1.006ProPhe: 1.006 ± 0.334
2.315ProGly: 2.315 ± 0.279
0.906ProHis: 0.906 ± 0.334
2.415ProIle: 2.415 ± 0.446
1.308ProLys: 1.308 ± 0.422
2.415ProLeu: 2.415 ± 0.554
0.906ProMet: 0.906 ± 0.296
1.006ProAsn: 1.006 ± 0.318
1.51ProPro: 1.51 ± 0.336
0.604ProGln: 0.604 ± 0.263
3.422ProArg: 3.422 ± 0.658
2.818ProSer: 2.818 ± 0.455
2.617ProThr: 2.617 ± 0.582
3.321ProVal: 3.321 ± 0.693
0.503ProTrp: 0.503 ± 0.185
1.006ProTyr: 1.006 ± 0.38
0.0ProXaa: 0.0 ± 0.0
Gln
4.026GlnAla: 4.026 ± 0.724
0.302GlnCys: 0.302 ± 0.176
1.107GlnAsp: 1.107 ± 0.405
1.812GlnGlu: 1.812 ± 0.369
1.812GlnPhe: 1.812 ± 0.569
3.019GlnGly: 3.019 ± 0.59
0.302GlnHis: 0.302 ± 0.145
1.812GlnIle: 1.812 ± 0.438
2.013GlnLys: 2.013 ± 0.596
4.63GlnLeu: 4.63 ± 0.827
0.302GlnMet: 0.302 ± 0.183
0.705GlnAsn: 0.705 ± 0.243
0.403GlnPro: 0.403 ± 0.188
1.308GlnGln: 1.308 ± 0.338
3.12GlnArg: 3.12 ± 0.566
2.214GlnSer: 2.214 ± 0.397
2.013GlnThr: 2.013 ± 0.534
1.51GlnVal: 1.51 ± 0.363
0.805GlnTrp: 0.805 ± 0.231
1.107GlnTyr: 1.107 ± 0.391
0.0GlnXaa: 0.0 ± 0.0
Arg
9.561ArgAla: 9.561 ± 1.5
0.705ArgCys: 0.705 ± 0.368
3.724ArgAsp: 3.724 ± 0.572
4.227ArgGlu: 4.227 ± 0.741
1.711ArgPhe: 1.711 ± 0.376
5.133ArgGly: 5.133 ± 1.002
1.409ArgHis: 1.409 ± 0.417
3.724ArgIle: 3.724 ± 0.749
4.63ArgLys: 4.63 ± 0.677
5.535ArgLeu: 5.535 ± 0.928
2.315ArgMet: 2.315 ± 0.594
2.617ArgAsn: 2.617 ± 0.503
3.12ArgPro: 3.12 ± 0.59
2.919ArgGln: 2.919 ± 0.716
5.032ArgArg: 5.032 ± 1.004
3.321ArgSer: 3.321 ± 0.615
3.724ArgThr: 3.724 ± 0.678
6.24ArgVal: 6.24 ± 0.801
0.302ArgTrp: 0.302 ± 0.182
1.409ArgTyr: 1.409 ± 0.41
0.0ArgXaa: 0.0 ± 0.0
Ser
8.655SerAla: 8.655 ± 0.881
0.705SerCys: 0.705 ± 0.324
2.415SerAsp: 2.415 ± 0.528
2.114SerGlu: 2.114 ± 0.458
2.214SerPhe: 2.214 ± 0.426
3.321SerGly: 3.321 ± 0.627
1.61SerHis: 1.61 ± 0.42
3.221SerIle: 3.221 ± 0.664
2.114SerLys: 2.114 ± 0.56
5.133SerLeu: 5.133 ± 0.641
1.308SerMet: 1.308 ± 0.343
1.61SerAsn: 1.61 ± 0.375
3.523SerPro: 3.523 ± 0.593
1.61SerGln: 1.61 ± 0.407
4.227SerArg: 4.227 ± 0.508
2.717SerSer: 2.717 ± 0.381
4.328SerThr: 4.328 ± 0.779
2.415SerVal: 2.415 ± 0.523
1.208SerTrp: 1.208 ± 0.398
0.705SerTyr: 0.705 ± 0.25
0.0SerXaa: 0.0 ± 0.0
Thr
6.441ThrAla: 6.441 ± 0.844
0.503ThrCys: 0.503 ± 0.196
4.026ThrAsp: 4.026 ± 0.772
3.12ThrGlu: 3.12 ± 0.617
2.617ThrPhe: 2.617 ± 0.595
4.63ThrGly: 4.63 ± 0.779
1.208ThrHis: 1.208 ± 0.327
3.724ThrIle: 3.724 ± 0.682
2.617ThrLys: 2.617 ± 0.533
5.938ThrLeu: 5.938 ± 0.932
1.61ThrMet: 1.61 ± 0.425
2.013ThrAsn: 2.013 ± 0.34
4.126ThrPro: 4.126 ± 0.661
2.013ThrGln: 2.013 ± 0.497
3.523ThrArg: 3.523 ± 0.55
3.321ThrSer: 3.321 ± 0.507
4.831ThrThr: 4.831 ± 0.975
4.73ThrVal: 4.73 ± 0.792
0.201ThrTrp: 0.201 ± 0.159
1.107ThrTyr: 1.107 ± 0.307
0.0ThrXaa: 0.0 ± 0.0
Val
9.863ValAla: 9.863 ± 1.085
0.302ValCys: 0.302 ± 0.191
4.529ValAsp: 4.529 ± 0.794
2.919ValGlu: 2.919 ± 0.546
2.214ValPhe: 2.214 ± 0.459
3.422ValGly: 3.422 ± 0.565
0.705ValHis: 0.705 ± 0.297
3.724ValIle: 3.724 ± 0.496
2.516ValLys: 2.516 ± 0.514
5.334ValLeu: 5.334 ± 0.632
2.013ValMet: 2.013 ± 0.487
2.516ValAsn: 2.516 ± 0.464
2.013ValPro: 2.013 ± 0.402
3.019ValGln: 3.019 ± 0.544
4.227ValArg: 4.227 ± 0.567
3.623ValSer: 3.623 ± 0.594
3.925ValThr: 3.925 ± 0.694
4.328ValVal: 4.328 ± 0.672
0.906ValTrp: 0.906 ± 0.344
1.308ValTyr: 1.308 ± 0.318
0.0ValXaa: 0.0 ± 0.0
Trp
1.308TrpAla: 1.308 ± 0.339
0.0TrpCys: 0.0 ± 0.0
0.503TrpAsp: 0.503 ± 0.22
0.906TrpGlu: 0.906 ± 0.253
0.906TrpPhe: 0.906 ± 0.32
0.805TrpGly: 0.805 ± 0.278
0.705TrpHis: 0.705 ± 0.257
0.403TrpIle: 0.403 ± 0.187
0.705TrpLys: 0.705 ± 0.218
1.51TrpLeu: 1.51 ± 0.338
0.0TrpMet: 0.0 ± 0.0
0.201TrpAsn: 0.201 ± 0.181
0.705TrpPro: 0.705 ± 0.286
0.302TrpGln: 0.302 ± 0.166
1.51TrpArg: 1.51 ± 0.384
0.604TrpSer: 0.604 ± 0.289
0.906TrpThr: 0.906 ± 0.326
1.006TrpVal: 1.006 ± 0.379
0.101TrpTrp: 0.101 ± 0.111
0.302TrpTyr: 0.302 ± 0.146
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.617TyrAla: 2.617 ± 0.598
0.201TyrCys: 0.201 ± 0.133
1.409TyrAsp: 1.409 ± 0.271
1.61TyrGlu: 1.61 ± 0.443
1.308TyrPhe: 1.308 ± 0.437
1.51TyrGly: 1.51 ± 0.393
1.006TyrHis: 1.006 ± 0.295
0.805TyrIle: 0.805 ± 0.296
0.503TyrLys: 0.503 ± 0.217
2.013TyrLeu: 2.013 ± 0.532
0.403TyrMet: 0.403 ± 0.234
0.604TyrAsn: 0.604 ± 0.26
0.906TyrPro: 0.906 ± 0.36
1.208TyrGln: 1.208 ± 0.351
1.711TyrArg: 1.711 ± 0.409
1.711TyrSer: 1.711 ± 0.408
1.51TyrThr: 1.51 ± 0.373
2.214TyrVal: 2.214 ± 0.496
0.403TyrTrp: 0.403 ± 0.168
0.403TyrTyr: 0.403 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (9937 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski