Raspberry latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; unclassified Reoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3VL88|E3VL88_9REOV Protein P7 OS=Raspberry latent virus OX=907191 PE=4 SV=1
MM1 pKa = 7.69ASYY4 pKa = 9.53TNYY7 pKa = 10.22MITFLNTVQRR17 pKa = 11.84IHH19 pKa = 6.84DD20 pKa = 3.7QNYY23 pKa = 10.35SDD25 pKa = 5.25FPPANVQVRR34 pKa = 11.84RR35 pKa = 11.84ITFQDD40 pKa = 3.18VEE42 pKa = 4.39EE43 pKa = 4.67YY44 pKa = 10.41IRR46 pKa = 11.84DD47 pKa = 3.57LPEE50 pKa = 4.19EE51 pKa = 4.19IKK53 pKa = 10.76DD54 pKa = 3.85YY55 pKa = 7.52TTKK58 pKa = 9.22TWNEE62 pKa = 4.01VQGEE66 pKa = 4.62SPMVIQIPFCTTNTRR81 pKa = 11.84QIRR84 pKa = 11.84NYY86 pKa = 10.18AAIVNLAYY94 pKa = 9.98SCSMAWPTPLPNGRR108 pKa = 11.84FNLEE112 pKa = 3.73DD113 pKa = 3.65VIAEE117 pKa = 4.25GFSSDD122 pKa = 3.9EE123 pKa = 3.75FLPTYY128 pKa = 9.81RR129 pKa = 11.84VPPAFVRR136 pKa = 11.84DD137 pKa = 4.06DD138 pKa = 3.96QPPSTSDD145 pKa = 3.02HH146 pKa = 6.52SPRR149 pKa = 11.84AQTPPEE155 pKa = 4.43GNDD158 pKa = 3.34FDD160 pKa = 5.56PDD162 pKa = 3.81PDD164 pKa = 3.92FVEE167 pKa = 4.46PAPVLAIVPHH177 pKa = 5.89RR178 pKa = 11.84HH179 pKa = 6.08LPNTDD184 pKa = 3.15QPVLPRR190 pKa = 11.84FGYY193 pKa = 10.1RR194 pKa = 11.84EE195 pKa = 3.96EE196 pKa = 4.59VRR198 pKa = 11.84DD199 pKa = 4.13DD200 pKa = 3.9DD201 pKa = 6.4ADD203 pKa = 3.74MFDD206 pKa = 4.84GDD208 pKa = 4.2DD209 pKa = 3.48ASEE212 pKa = 4.02VGEE215 pKa = 4.03VGEE218 pKa = 4.24EE219 pKa = 4.0EE220 pKa = 5.2EE221 pKa = 4.6YY222 pKa = 11.24LDD224 pKa = 4.89PLPEE228 pKa = 3.82QDD230 pKa = 4.5RR231 pKa = 11.84EE232 pKa = 3.95NLALVRR238 pKa = 11.84SRR240 pKa = 11.84VVKK243 pKa = 10.77SLFDD247 pKa = 3.55RR248 pKa = 11.84KK249 pKa = 10.42KK250 pKa = 9.14VGEE253 pKa = 4.17VDD255 pKa = 3.44SVMMRR260 pKa = 11.84PRR262 pKa = 11.84FNPGVVPRR270 pKa = 11.84YY271 pKa = 8.38GTVKK275 pKa = 9.55HH276 pKa = 5.58YY277 pKa = 10.08EE278 pKa = 4.19YY279 pKa = 10.86KK280 pKa = 10.67EE281 pKa = 4.13EE282 pKa = 5.01PDD284 pKa = 3.88SGWLSEE290 pKa = 4.8AGDD293 pKa = 4.0SLVVMNGLVQGDD305 pKa = 5.17PIPITDD311 pKa = 3.71IDD313 pKa = 6.09GIIIWNARR321 pKa = 11.84EE322 pKa = 3.88DD323 pKa = 3.82ADD325 pKa = 3.96VNQRR329 pKa = 11.84DD330 pKa = 4.08FTVLEE335 pKa = 4.14GDD337 pKa = 3.93VFSMNPRR344 pKa = 11.84ILSGEE349 pKa = 4.56FYY351 pKa = 10.12QEE353 pKa = 3.56RR354 pKa = 11.84VNVEE358 pKa = 3.65RR359 pKa = 11.84NLRR362 pKa = 11.84LILLGNCLDD371 pKa = 3.53KK372 pKa = 11.16RR373 pKa = 11.84LVEE376 pKa = 4.19EE377 pKa = 4.14TDD379 pKa = 3.8RR380 pKa = 11.84ACSNYY385 pKa = 10.55LIGTQDD391 pKa = 4.73DD392 pKa = 4.25IVNEE396 pKa = 4.13ANTDD400 pKa = 3.03QDD402 pKa = 3.33RR403 pKa = 11.84NEE405 pKa = 4.32RR406 pKa = 11.84VCVMTSTGLICSSYY420 pKa = 9.13YY421 pKa = 10.46SCPAKK426 pKa = 10.23HH427 pKa = 6.41RR428 pKa = 11.84NKK430 pKa = 10.14IIPIEE435 pKa = 3.87IGRR438 pKa = 11.84RR439 pKa = 11.84VATRR443 pKa = 11.84LRR445 pKa = 11.84RR446 pKa = 11.84IIAMMVEE453 pKa = 4.58GDD455 pKa = 3.57QRR457 pKa = 11.84SVEE460 pKa = 4.13VLLMYY465 pKa = 9.82WMRR468 pKa = 11.84IIYY471 pKa = 9.2PDD473 pKa = 3.19TYY475 pKa = 11.04SPVRR479 pKa = 11.84NHH481 pKa = 5.25ITVGDD486 pKa = 3.99FTWARR491 pKa = 11.84SPVTRR496 pKa = 11.84DD497 pKa = 3.17FEE499 pKa = 5.68IITPYY504 pKa = 11.1LEE506 pKa = 4.24GTNDD510 pKa = 4.53DD511 pKa = 5.19DD512 pKa = 5.62GAVQNPEE519 pKa = 3.14RR520 pKa = 11.84WYY522 pKa = 9.34PSIFNPHH529 pKa = 6.23NLFQALNSFPLFLLILDD546 pKa = 3.75QCSAEE551 pKa = 3.92PSLEE555 pKa = 3.9FEE557 pKa = 4.62VLSEE561 pKa = 4.44RR562 pKa = 11.84LNEE565 pKa = 3.85LQLAYY570 pKa = 10.44FKK572 pKa = 11.21VIFHH576 pKa = 6.59CTTLNYY582 pKa = 10.06VLSNNPCSNMNLAIGPYY599 pKa = 9.13PMVYY603 pKa = 10.55QKK605 pKa = 9.94MRR607 pKa = 11.84DD608 pKa = 3.53YY609 pKa = 11.38AYY611 pKa = 8.59MVCKK615 pKa = 10.53SSVQAEE621 pKa = 4.08KK622 pKa = 11.25AFIYY626 pKa = 10.34LILGLLGFAA635 pKa = 5.13

Molecular weight: 72.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3VL92|E3VL92_9REOV Protein P10b OS=Raspberry latent virus OX=907191 PE=4 SV=1
MM1 pKa = 7.11MANTLTIDD9 pKa = 3.41SLTPTLEE16 pKa = 3.89TAYY19 pKa = 10.21GATSQTPRR27 pKa = 11.84NPTNILAILFDD38 pKa = 3.93IFKK41 pKa = 10.55HH42 pKa = 5.18PARR45 pKa = 11.84PQPRR49 pKa = 11.84RR50 pKa = 11.84AGHH53 pKa = 6.71AGNPGNKK60 pKa = 8.32STSLALLVTLEE71 pKa = 4.19QSTSLHH77 pKa = 5.58QALLQYY83 pKa = 11.01ASRR86 pKa = 11.84WPNALLAEE94 pKa = 4.34LTSLLRR100 pKa = 11.84EE101 pKa = 4.29TNRR104 pKa = 11.84LAQKK108 pKa = 10.36DD109 pKa = 4.15GRR111 pKa = 11.84CFITPIFMGKK121 pKa = 9.23LRR123 pKa = 11.84NLVQNQLANEE133 pKa = 4.56LSAIQYY139 pKa = 9.76RR140 pKa = 11.84RR141 pKa = 11.84ALDD144 pKa = 3.72LFVMRR149 pKa = 11.84SAPPEE154 pKa = 3.84FTYY157 pKa = 11.09NPAQNRR163 pKa = 11.84IALTDD168 pKa = 3.37TSMKK172 pKa = 10.51LIYY175 pKa = 9.87QEE177 pKa = 3.67PARR180 pKa = 11.84RR181 pKa = 11.84RR182 pKa = 11.84IADD185 pKa = 3.5TAFNWFGMDD194 pKa = 3.51YY195 pKa = 10.91GVVPYY200 pKa = 10.46RR201 pKa = 11.84CGNQTYY207 pKa = 10.56AEE209 pKa = 4.57DD210 pKa = 4.31GANILHH216 pKa = 6.83PKK218 pKa = 9.88AHH220 pKa = 7.0LLKK223 pKa = 10.35QAYY226 pKa = 8.92AVQNAALAEE235 pKa = 4.06LMVQSSPFQTIPQSIPEE252 pKa = 4.24GQFTLAACPMTNRR265 pKa = 11.84ILKK268 pKa = 10.12DD269 pKa = 2.95SHH271 pKa = 6.37LWYY274 pKa = 10.49RR275 pKa = 11.84KK276 pKa = 7.49TWDD279 pKa = 2.95QDD281 pKa = 2.69TRR283 pKa = 11.84QRR285 pKa = 11.84VNPGIWNVDD294 pKa = 3.59LDD296 pKa = 4.04DD297 pKa = 4.6QSWNTAPHH305 pKa = 6.39SFGFPDD311 pKa = 4.87SMTSDD316 pKa = 3.32KK317 pKa = 11.34LKK319 pKa = 9.78WQYY322 pKa = 11.11AQYY325 pKa = 11.09FVMDD329 pKa = 4.2YY330 pKa = 10.78INTYY334 pKa = 7.15TALYY338 pKa = 8.83VVNVV342 pKa = 3.68

Molecular weight: 38.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

8720

200

1276

726.7

81.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.032 ± 0.22

1.72 ± 0.226

5.459 ± 0.276

5.963 ± 0.238

4.094 ± 0.141

5.539 ± 0.352

2.041 ± 0.122

6.135 ± 0.158

4.541 ± 0.375

8.842 ± 0.498

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.833 ± 0.133

4.702 ± 0.316

5.55 ± 0.36

4.163 ± 0.238

6.021 ± 0.3

8.417 ± 0.659

6.239 ± 0.299

6.927 ± 0.267

1.227 ± 0.07

3.555 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski