Ensifer sp. LCM 4579

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Ensifer; unclassified Ensifer

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5430 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S1SQC4|A0A1S1SQC4_9RHIZ Dehydrogenase OS=Ensifer sp. LCM 4579 OX=1848292 GN=LCM4579_08300 PE=3 SV=1
MM1 pKa = 7.78ADD3 pKa = 3.81SRR5 pKa = 11.84PLSDD9 pKa = 5.41DD10 pKa = 3.74APNTNTDD17 pKa = 2.89QSTVAAGHH25 pKa = 6.71LALVPTWLPFDD36 pKa = 3.47IAGDD40 pKa = 3.77SGQNAAGNGGDD51 pKa = 3.73GTSVGIVGSNTLAMFVPSNMAIAGPQSNAGAEE83 pKa = 4.06QGNNALIYY91 pKa = 9.39QHH93 pKa = 5.91ATEE96 pKa = 4.15MAGIGGDD103 pKa = 3.44GGSGNHH109 pKa = 7.28AIGSVGNANLAGNGGNGTFFGSLLSTDD136 pKa = 3.75VAVFAPVNTAVAAGPGATATAEE158 pKa = 4.17QTNNAAILQGATQVGGVGGAGGDD181 pKa = 3.68HH182 pKa = 6.68NSAGGAPAASAGATFTFTGDD202 pKa = 2.79NYY204 pKa = 10.87AGHH207 pKa = 6.9GGDD210 pKa = 3.38GTFIGSMVDD219 pKa = 3.07VNVAVFSPINIAVGAAGGNAQATQTNNVIFDD250 pKa = 3.46QGGVQIAGIGGNGGGFNLSSDD271 pKa = 4.01TIFTGNAVGAGGGGGTGHH289 pKa = 6.0STGSLVDD296 pKa = 3.42VNIGYY301 pKa = 7.43FHH303 pKa = 7.46PVNIAVPAGGTAEE316 pKa = 4.54ADD318 pKa = 3.53QLNHH322 pKa = 5.96VLYY325 pKa = 10.49DD326 pKa = 3.32QHH328 pKa = 6.26TLQLGGIGGAGGHH341 pKa = 6.08GNIADD346 pKa = 4.13ANSSLVDD353 pKa = 3.79DD354 pKa = 4.51VLALVNAA361 pKa = 5.04

Molecular weight: 34.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S1SZX3|A0A1S1SZX3_9RHIZ Molybdopterin-guanine dinucleotide biosynthesis protein B OS=Ensifer sp. LCM 4579 OX=1848292 GN=LCM4579_05235 PE=4 SV=1
MM1 pKa = 7.88PSFLTAVTLFVMGLVAIVLIRR22 pKa = 11.84GLLNMARR29 pKa = 11.84RR30 pKa = 11.84GSGNTSNKK38 pKa = 8.96LMQMRR43 pKa = 11.84ILLQAVALVLIMLTLWITGGGRR65 pKa = 11.84PAA67 pKa = 4.25

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5430

0

5430

1696053

28

2870

312.3

34.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.198 ± 0.046

0.875 ± 0.011

5.548 ± 0.024

6.155 ± 0.036

3.912 ± 0.022

8.362 ± 0.032

2.064 ± 0.018

5.519 ± 0.022

3.412 ± 0.026

10.046 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.014

2.661 ± 0.017

4.934 ± 0.022

2.946 ± 0.018

7.209 ± 0.039

5.586 ± 0.025

5.14 ± 0.022

7.36 ± 0.024

1.287 ± 0.013

2.289 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski