Amino acid dipepetide frequency for Ensifer sp. LCM 4579

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.895AlaAla: 16.895 ± 0.174	1.096AlaCys: 1.096 ± 0.03	6.565AlaAsp: 6.565 ± 0.065	8.129AlaGlu: 8.129 ± 0.085	4.673AlaPhe: 4.673 ± 0.057	10.384AlaGly: 10.384 ± 0.088	2.177AlaHis: 2.177 ± 0.042	6.953AlaIle: 6.953 ± 0.068	4.023AlaLys: 4.023 ± 0.06	12.988AlaLeu: 12.988 ± 0.121	3.404AlaMet: 3.404 ± 0.051	2.943AlaAsn: 2.943 ± 0.04	4.87AlaPro: 4.87 ± 0.062	3.323AlaGln: 3.323 ± 0.059	8.563AlaArg: 8.563 ± 0.074	6.389AlaSer: 6.389 ± 0.069	5.742AlaThr: 5.742 ± 0.061	8.846AlaVal: 8.846 ± 0.072	1.415AlaTrp: 1.415 ± 0.029	2.6AlaTyr: 2.6 ± 0.04	0.001AlaXaa: 0.001 ± 0.001
Cys	0.953CysAla: 0.953 ± 0.028	0.129CysCys: 0.129 ± 0.009	0.511CysAsp: 0.511 ± 0.017	0.482CysGlu: 0.482 ± 0.018	0.367CysPhe: 0.367 ± 0.016	1.0CysGly: 1.0 ± 0.027	0.254CysHis: 0.254 ± 0.012	0.423CysIle: 0.423 ± 0.017	0.202CysLys: 0.202 ± 0.011	0.855CysLeu: 0.855 ± 0.026	0.17CysMet: 0.17 ± 0.009	0.224CysAsn: 0.224 ± 0.012	0.439CysPro: 0.439 ± 0.017	0.242CysGln: 0.242 ± 0.013	0.66CysArg: 0.66 ± 0.02	0.502CysSer: 0.502 ± 0.018	0.402CysThr: 0.402 ± 0.017	0.607CysVal: 0.607 ± 0.02	0.114CysTrp: 0.114 ± 0.009	0.213CysTyr: 0.213 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.27AspAla: 6.27 ± 0.069	0.539AspCys: 0.539 ± 0.018	2.952AspAsp: 2.952 ± 0.055	3.671AspGlu: 3.671 ± 0.048	2.304AspPhe: 2.304 ± 0.038	5.01AspGly: 5.01 ± 0.061	1.241AspHis: 1.241 ± 0.032	3.226AspIle: 3.226 ± 0.044	1.58AspLys: 1.58 ± 0.034	5.725AspLeu: 5.725 ± 0.07	1.317AspMet: 1.317 ± 0.031	1.264AspAsn: 1.264 ± 0.028	3.355AspPro: 3.355 ± 0.047	1.61AspGln: 1.61 ± 0.036	4.419AspArg: 4.419 ± 0.05	2.081AspSer: 2.081 ± 0.037	2.369AspThr: 2.369 ± 0.039	4.122AspVal: 4.122 ± 0.048	0.913AspTrp: 0.913 ± 0.021	1.515AspTyr: 1.515 ± 0.029	0.001AspXaa: 0.001 ± 0.001
Glu	7.981GluAla: 7.981 ± 0.088	0.413GluCys: 0.413 ± 0.014	2.999GluAsp: 2.999 ± 0.037	3.996GluGlu: 3.996 ± 0.065	1.996GluPhe: 1.996 ± 0.038	4.518GluGly: 4.518 ± 0.049	1.258GluHis: 1.258 ± 0.028	4.015GluIle: 4.015 ± 0.051	2.654GluLys: 2.654 ± 0.044	5.673GluLeu: 5.673 ± 0.055	1.631GluMet: 1.631 ± 0.027	1.853GluAsn: 1.853 ± 0.039	2.789GluPro: 2.789 ± 0.04	2.141GluGln: 2.141 ± 0.04	5.46GluArg: 5.46 ± 0.068	2.61GluSer: 2.61 ± 0.039	3.756GluThr: 3.756 ± 0.045	3.956GluVal: 3.956 ± 0.045	0.751GluTrp: 0.751 ± 0.023	1.097GluTyr: 1.097 ± 0.026	0.0GluXaa: 0.0 ± 0.0
Phe	4.7PheAla: 4.7 ± 0.06	0.409PheCys: 0.409 ± 0.016	2.776PheAsp: 2.776 ± 0.045	2.377PheGlu: 2.377 ± 0.039	1.634PhePhe: 1.634 ± 0.043	3.655PheGly: 3.655 ± 0.044	0.816PheHis: 0.816 ± 0.023	1.826PheIle: 1.826 ± 0.042	1.068PheLys: 1.068 ± 0.028	3.743PheLeu: 3.743 ± 0.06	0.845PheMet: 0.845 ± 0.025	1.078PheAsn: 1.078 ± 0.027	1.657PhePro: 1.657 ± 0.03	1.048PheGln: 1.048 ± 0.024	2.547PheArg: 2.547 ± 0.036	2.416PheSer: 2.416 ± 0.04	1.961PheThr: 1.961 ± 0.037	3.014PheVal: 3.014 ± 0.044	0.573PheTrp: 0.573 ± 0.021	0.975PheTyr: 0.975 ± 0.023	0.001PheXaa: 0.001 ± 0.001
Gly	8.79GlyAla: 8.79 ± 0.082	0.873GlyCys: 0.873 ± 0.025	4.345GlyAsp: 4.345 ± 0.057	5.064GlyGlu: 5.064 ± 0.056	3.633GlyPhe: 3.633 ± 0.046	7.072GlyGly: 7.072 ± 0.093	1.897GlyHis: 1.897 ± 0.038	4.77GlyIle: 4.77 ± 0.056	3.379GlyLys: 3.379 ± 0.055	8.63GlyLeu: 8.63 ± 0.068	2.272GlyMet: 2.272 ± 0.035	2.265GlyAsn: 2.265 ± 0.037	3.238GlyPro: 3.238 ± 0.048	2.541GlyGln: 2.541 ± 0.038	6.267GlyArg: 6.267 ± 0.069	4.689GlySer: 4.689 ± 0.056	4.403GlyThr: 4.403 ± 0.063	5.871GlyVal: 5.871 ± 0.065	1.233GlyTrp: 1.233 ± 0.027	2.38GlyTyr: 2.38 ± 0.044	0.002GlyXaa: 0.002 ± 0.001
His	2.334HisAla: 2.334 ± 0.037	0.215HisCys: 0.215 ± 0.013	1.155HisAsp: 1.155 ± 0.031	1.122HisGlu: 1.122 ± 0.027	0.96HisPhe: 0.96 ± 0.022	1.896HisGly: 1.896 ± 0.036	0.558HisHis: 0.558 ± 0.02	0.989HisIle: 0.989 ± 0.022	0.505HisLys: 0.505 ± 0.016	2.143HisLeu: 2.143 ± 0.038	0.54HisMet: 0.54 ± 0.015	0.463HisAsn: 0.463 ± 0.017	1.306HisPro: 1.306 ± 0.029	0.606HisGln: 0.606 ± 0.02	1.554HisArg: 1.554 ± 0.034	1.008HisSer: 1.008 ± 0.026	0.775HisThr: 0.775 ± 0.019	1.63HisVal: 1.63 ± 0.033	0.307HisTrp: 0.307 ± 0.013	0.577HisTyr: 0.577 ± 0.02	0.001HisXaa: 0.001 ± 0.001
Ile	7.851IleAla: 7.851 ± 0.083	0.577IleCys: 0.577 ± 0.02	3.786IleAsp: 3.786 ± 0.048	3.883IleGlu: 3.883 ± 0.044	1.974IlePhe: 1.974 ± 0.035	5.324IleGly: 5.324 ± 0.061	1.012IleHis: 1.012 ± 0.026	2.511IleIle: 2.511 ± 0.046	1.486IleLys: 1.486 ± 0.029	4.795IleLeu: 4.795 ± 0.057	1.065IleMet: 1.065 ± 0.024	1.505IleAsn: 1.505 ± 0.03	2.368IlePro: 2.368 ± 0.038	1.172IleGln: 1.172 ± 0.03	3.687IleArg: 3.687 ± 0.048	3.148IleSer: 3.148 ± 0.05	2.666IleThr: 2.666 ± 0.043	4.526IleVal: 4.526 ± 0.057	0.617IleTrp: 0.617 ± 0.021	1.233IleTyr: 1.233 ± 0.029	0.002IleXaa: 0.002 ± 0.001
Lys	4.493LysAla: 4.493 ± 0.064	0.178LysCys: 0.178 ± 0.011	1.779LysAsp: 1.779 ± 0.037	1.979LysGlu: 1.979 ± 0.038	0.917LysPhe: 0.917 ± 0.023	2.73LysGly: 2.73 ± 0.047	0.664LysHis: 0.664 ± 0.021	1.886LysIle: 1.886 ± 0.032	1.345LysLys: 1.345 ± 0.034	3.374LysLeu: 3.374 ± 0.051	0.802LysMet: 0.802 ± 0.024	0.943LysAsn: 0.943 ± 0.028	2.028LysPro: 2.028 ± 0.034	1.012LysGln: 1.012 ± 0.028	2.492LysArg: 2.492 ± 0.041	2.028LysSer: 2.028 ± 0.037	1.986LysThr: 1.986 ± 0.039	2.427LysVal: 2.427 ± 0.044	0.404LysTrp: 0.404 ± 0.015	0.654LysTyr: 0.654 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	13.197LeuAla: 13.197 ± 0.115	0.921LeuCys: 0.921 ± 0.027	5.809LeuAsp: 5.809 ± 0.063	5.684LeuGlu: 5.684 ± 0.066	3.865LeuPhe: 3.865 ± 0.054	7.906LeuGly: 7.906 ± 0.077	1.805LeuHis: 1.805 ± 0.036	5.177LeuIle: 5.177 ± 0.057	3.976LeuLys: 3.976 ± 0.055	9.389LeuLeu: 9.389 ± 0.107	2.384LeuMet: 2.384 ± 0.039	2.577LeuAsn: 2.577 ± 0.036	5.342LeuPro: 5.342 ± 0.063	2.766LeuGln: 2.766 ± 0.046	6.616LeuArg: 6.616 ± 0.075	6.897LeuSer: 6.897 ± 0.096	5.421LeuThr: 5.421 ± 0.067	7.467LeuVal: 7.467 ± 0.073	1.108LeuTrp: 1.108 ± 0.03	2.154LeuTyr: 2.154 ± 0.038	0.001LeuXaa: 0.001 ± 0.001
Met	3.091MetAla: 3.091 ± 0.047	0.155MetCys: 0.155 ± 0.009	1.097MetAsp: 1.097 ± 0.027	1.247MetGlu: 1.247 ± 0.024	0.694MetPhe: 0.694 ± 0.021	1.666MetGly: 1.666 ± 0.033	0.475MetHis: 0.475 ± 0.016	1.45MetIle: 1.45 ± 0.033	1.105MetLys: 1.105 ± 0.031	2.59MetLeu: 2.59 ± 0.043	0.656MetMet: 0.656 ± 0.025	0.862MetAsn: 0.862 ± 0.025	1.489MetPro: 1.489 ± 0.029	0.832MetGln: 0.832 ± 0.023	1.88MetArg: 1.88 ± 0.039	1.683MetSer: 1.683 ± 0.031	1.808MetThr: 1.808 ± 0.039	1.691MetVal: 1.691 ± 0.03	0.192MetTrp: 0.192 ± 0.011	0.302MetTyr: 0.302 ± 0.013	0.001MetXaa: 0.001 ± 0.001
Asn	3.222AsnAla: 3.222 ± 0.046	0.251AsnCys: 0.251 ± 0.014	1.424AsnAsp: 1.424 ± 0.03	1.381AsnGlu: 1.381 ± 0.031	1.015AsnPhe: 1.015 ± 0.028	2.489AsnGly: 2.489 ± 0.042	0.519AsnHis: 0.519 ± 0.019	1.487AsnIle: 1.487 ± 0.033	0.68AsnLys: 0.68 ± 0.021	2.578AsnLeu: 2.578 ± 0.042	0.637AsnMet: 0.637 ± 0.02	0.708AsnAsn: 0.708 ± 0.022	1.81AsnPro: 1.81 ± 0.032	0.74AsnGln: 0.74 ± 0.021	2.003AsnArg: 2.003 ± 0.036	1.293AsnSer: 1.293 ± 0.03	1.266AsnThr: 1.266 ± 0.027	1.946AsnVal: 1.946 ± 0.036	0.46AsnTrp: 0.46 ± 0.016	0.695AsnTyr: 0.695 ± 0.022	0.001AsnXaa: 0.001 ± 0.001
Pro	5.749ProAla: 5.749 ± 0.072	0.302ProCys: 0.302 ± 0.014	3.384ProAsp: 3.384 ± 0.052	3.789ProGlu: 3.789 ± 0.045	1.993ProPhe: 1.993 ± 0.032	4.149ProGly: 4.149 ± 0.052	1.122ProHis: 1.122 ± 0.024	2.386ProIle: 2.386 ± 0.039	1.688ProLys: 1.688 ± 0.032	4.7ProLeu: 4.7 ± 0.063	1.132ProMet: 1.132 ± 0.026	1.304ProAsn: 1.304 ± 0.028	2.318ProPro: 2.318 ± 0.044	1.551ProGln: 1.551 ± 0.031	2.755ProArg: 2.755 ± 0.042	2.768ProSer: 2.768 ± 0.04	2.284ProThr: 2.284 ± 0.038	4.163ProVal: 4.163 ± 0.049	0.643ProTrp: 0.643 ± 0.017	1.16ProTyr: 1.16 ± 0.028	0.001ProXaa: 0.001 ± 0.001
Gln	3.702GlnAla: 3.702 ± 0.048	0.199GlnCys: 0.199 ± 0.01	1.271GlnAsp: 1.271 ± 0.026	1.631GlnGlu: 1.631 ± 0.034	1.103GlnPhe: 1.103 ± 0.023	2.081GlnGly: 2.081 ± 0.034	0.611GlnHis: 0.611 ± 0.021	1.841GlnIle: 1.841 ± 0.062	1.117GlnLys: 1.117 ± 0.026	2.718GlnLeu: 2.718 ± 0.043	0.897GlnMet: 0.897 ± 0.025	0.779GlnAsn: 0.779 ± 0.021	1.629GlnPro: 1.629 ± 0.033	1.205GlnGln: 1.205 ± 0.031	2.292GlnArg: 2.292 ± 0.037	1.776GlnSer: 1.776 ± 0.034	1.61GlnThr: 1.61 ± 0.034	2.047GlnVal: 2.047 ± 0.044	0.384GlnTrp: 0.384 ± 0.015	0.573GlnTyr: 0.573 ± 0.02	0.001GlnXaa: 0.001 ± 0.001
Arg	7.658ArgAla: 7.658 ± 0.065	0.541ArgCys: 0.541 ± 0.018	3.945ArgAsp: 3.945 ± 0.053	4.393ArgGlu: 4.393 ± 0.056	3.032ArgPhe: 3.032 ± 0.044	4.791ArgGly: 4.791 ± 0.06	1.833ArgHis: 1.833 ± 0.034	4.271ArgIle: 4.271 ± 0.051	2.57ArgLys: 2.57 ± 0.039	8.041ArgLeu: 8.041 ± 0.08	1.957ArgMet: 1.957 ± 0.032	2.055ArgAsn: 2.055 ± 0.036	3.563ArgPro: 3.563 ± 0.058	2.717ArgGln: 2.717 ± 0.045	5.97ArgArg: 5.97 ± 0.07	4.008ArgSer: 4.008 ± 0.052	3.412ArgThr: 3.412 ± 0.046	4.591ArgVal: 4.591 ± 0.051	0.947ArgTrp: 0.947 ± 0.024	1.789ArgTyr: 1.789 ± 0.036	0.001ArgXaa: 0.001 ± 0.001
Ser	6.375SerAla: 6.375 ± 0.061	0.43SerCys: 0.43 ± 0.016	3.039SerAsp: 3.039 ± 0.047	3.263SerGlu: 3.263 ± 0.043	2.433SerPhe: 2.433 ± 0.038	5.579SerGly: 5.579 ± 0.067	1.161SerHis: 1.161 ± 0.028	3.043SerIle: 3.043 ± 0.043	1.712SerLys: 1.712 ± 0.033	5.682SerLeu: 5.682 ± 0.062	1.432SerMet: 1.432 ± 0.029	1.43SerAsn: 1.43 ± 0.029	2.776SerPro: 2.776 ± 0.04	1.584SerGln: 1.584 ± 0.034	3.845SerArg: 3.845 ± 0.048	3.11SerSer: 3.11 ± 0.059	2.722SerThr: 2.722 ± 0.082	4.143SerVal: 4.143 ± 0.048	0.75SerTrp: 0.75 ± 0.023	1.348SerTyr: 1.348 ± 0.028	0.001SerXaa: 0.001 ± 0.001
Thr	6.108ThrAla: 6.108 ± 0.08	0.44ThrCys: 0.44 ± 0.017	2.627ThrAsp: 2.627 ± 0.039	2.829ThrGlu: 2.829 ± 0.048	2.029ThrPhe: 2.029 ± 0.036	4.957ThrGly: 4.957 ± 0.054	0.999ThrHis: 0.999 ± 0.023	3.117ThrIle: 3.117 ± 0.045	1.492ThrLys: 1.492 ± 0.034	5.408ThrLeu: 5.408 ± 0.058	1.228ThrMet: 1.228 ± 0.027	1.298ThrAsn: 1.298 ± 0.031	2.88ThrPro: 2.88 ± 0.039	1.235ThrGln: 1.235 ± 0.03	3.203ThrArg: 3.203 ± 0.044	2.808ThrSer: 2.808 ± 0.048	2.649ThrThr: 2.649 ± 0.048	4.291ThrVal: 4.291 ± 0.055	0.634ThrTrp: 0.634 ± 0.018	1.167ThrTyr: 1.167 ± 0.029	0.002ThrXaa: 0.002 ± 0.001
Val	8.948ValAla: 8.948 ± 0.074	0.657ValCys: 0.657 ± 0.021	4.043ValAsp: 4.043 ± 0.058	4.703ValGlu: 4.703 ± 0.053	2.932ValPhe: 2.932 ± 0.051	5.44ValGly: 5.44 ± 0.063	1.424ValHis: 1.424 ± 0.028	4.144ValIle: 4.144 ± 0.053	2.407ValLys: 2.407 ± 0.041	7.501ValLeu: 7.501 ± 0.082	1.782ValMet: 1.782 ± 0.034	2.008ValAsn: 2.008 ± 0.035	3.691ValPro: 3.691 ± 0.048	1.887ValGln: 1.887 ± 0.035	4.864ValArg: 4.864 ± 0.054	4.575ValSer: 4.575 ± 0.055	4.444ValThr: 4.444 ± 0.052	5.791ValVal: 5.791 ± 0.059	0.839ValTrp: 0.839 ± 0.025	1.515ValTyr: 1.515 ± 0.031	0.002ValXaa: 0.002 ± 0.001
Trp	1.205TrpAla: 1.205 ± 0.025	0.144TrpCys: 0.144 ± 0.009	0.578TrpAsp: 0.578 ± 0.019	0.585TrpGlu: 0.585 ± 0.02	0.538TrpPhe: 0.538 ± 0.017	0.808TrpGly: 0.808 ± 0.024	0.315TrpHis: 0.315 ± 0.014	0.706TrpIle: 0.706 ± 0.022	0.509TrpLys: 0.509 ± 0.019	1.601TrpLeu: 1.601 ± 0.031	0.353TrpMet: 0.353 ± 0.016	0.461TrpAsn: 0.461 ± 0.018	0.654TrpPro: 0.654 ± 0.02	0.551TrpGln: 0.551 ± 0.016	1.058TrpArg: 1.058 ± 0.025	0.803TrpSer: 0.803 ± 0.021	0.724TrpThr: 0.724 ± 0.023	0.759TrpVal: 0.759 ± 0.021	0.215TrpTrp: 0.215 ± 0.012	0.305TrpTyr: 0.305 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.454TyrAla: 2.454 ± 0.043	0.279TyrCys: 0.279 ± 0.013	1.397TyrAsp: 1.397 ± 0.032	1.335TyrGlu: 1.335 ± 0.027	1.026TyrPhe: 1.026 ± 0.025	2.161TyrGly: 2.161 ± 0.038	0.502TyrHis: 0.502 ± 0.017	0.969TyrIle: 0.969 ± 0.027	0.623TyrLys: 0.623 ± 0.02	2.327TyrLeu: 2.327 ± 0.037	0.458TyrMet: 0.458 ± 0.015	0.584TyrAsn: 0.584 ± 0.019	1.137TyrPro: 1.137 ± 0.025	0.702TyrGln: 0.702 ± 0.021	1.949TyrArg: 1.949 ± 0.039	1.266TyrSer: 1.266 ± 0.03	1.001TyrThr: 1.001 ± 0.026	1.71TyrVal: 1.71 ± 0.031	0.37TyrTrp: 0.37 ± 0.015	0.636TyrTyr: 0.636 ± 0.023	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.002XaaGlu: 0.002 ± 0.001	0.001XaaPhe: 0.001 ± 0.001	0.001XaaGly: 0.001 ± 0.001	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.002XaaMet: 0.002 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.002XaaGln: 0.002 ± 0.001	0.002XaaArg: 0.002 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 5430 proteins (1696053 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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