Lentibacter algarum
Average proteome isoelectric point is 6.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3261 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3NR98|A0A1H3NR98_9RHOB Uncharacterized protein OS=Lentibacter algarum OX=576131 GN=SAMN05444486_1174 PE=4 SV=1
MM1 pKa = 7.61 QYY3 pKa = 10.43 ILKK6 pKa = 9.44 PKK8 pKa = 8.68 WKK10 pKa = 9.9 KK11 pKa = 9.69 CVCVEE16 pKa = 4.19 TQWAHH21 pKa = 5.73 NDD23 pKa = 3.42 EE24 pKa = 4.6 PNRR27 pKa = 11.84 NAINSEE33 pKa = 4.01 YY34 pKa = 10.38 YY35 pKa = 9.83 RR36 pKa = 11.84 YY37 pKa = 10.6 EE38 pKa = 3.94 EE39 pKa = 4.58 FEE41 pKa = 4.19 VTLAEE46 pKa = 4.39 GVDD49 pKa = 3.72 VEE51 pKa = 5.32 VLKK54 pKa = 9.87 TWDD57 pKa = 3.93 EE58 pKa = 3.97 FDD60 pKa = 5.41 LDD62 pKa = 4.28 DD63 pKa = 4.74 EE64 pKa = 4.74 EE65 pKa = 5.12 TFASYY70 pKa = 10.36 EE71 pKa = 4.03 WLDD74 pKa = 3.66 TSPVSGDD81 pKa = 3.16 VTYY84 pKa = 11.36 SDD86 pKa = 3.77 WEE88 pKa = 4.25 VSNDD92 pKa = 3.37 CTDD95 pKa = 3.6 EE96 pKa = 3.8 EE97 pKa = 4.66 RR98 pKa = 11.84 EE99 pKa = 4.25 AIEE102 pKa = 6.06 EE103 pKa = 3.97 GDD105 pKa = 3.16 IWNLEE110 pKa = 3.85 DD111 pKa = 3.47 WDD113 pKa = 4.13 TGKK116 pKa = 10.88 SYY118 pKa = 10.49 TKK120 pKa = 10.19 INCEE124 pKa = 4.16 CEE126 pKa = 3.68 ITPASS131 pKa = 3.53
Molecular weight: 15.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.643
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.668
Grimsley 3.554
Solomon 3.783
Lehninger 3.745
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.681
EMBOSS 3.706
Sillero 3.948
Patrickios 1.062
IPC_peptide 3.795
IPC2_peptide 3.935
IPC2.peptide.svr19 3.838
Protein with the highest isoelectric point:
>tr|A0A1H3ILE0|A0A1H3ILE0_9RHOB Ribonuclease E OS=Lentibacter algarum OX=576131 GN=rne PE=3 SV=1
MM1 pKa = 7.26 NANRR5 pKa = 11.84 LINMVLRR12 pKa = 11.84 MVMRR16 pKa = 11.84 KK17 pKa = 8.7 VVSAGLKK24 pKa = 9.41 RR25 pKa = 11.84 AGGGKK30 pKa = 9.52 GASAATKK37 pKa = 10.16 GLNKK41 pKa = 9.68 ASRR44 pKa = 11.84 MARR47 pKa = 11.84 RR48 pKa = 11.84 AGRR51 pKa = 11.84 FF52 pKa = 3.17
Molecular weight: 5.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.574
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.31
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3261
0
3261
1000721
30
2360
306.9
33.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.093 ± 0.067
0.9 ± 0.014
5.639 ± 0.048
6.342 ± 0.04
3.855 ± 0.03
8.535 ± 0.047
2.052 ± 0.022
5.243 ± 0.029
3.876 ± 0.04
10.119 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.898 ± 0.022
2.818 ± 0.026
4.621 ± 0.032
3.202 ± 0.023
5.992 ± 0.039
5.588 ± 0.03
5.461 ± 0.036
7.097 ± 0.032
1.332 ± 0.02
2.335 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here