Polyomavirus sp.
Average proteome isoelectric point is 5.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8QQF4|A0A1D8QQF4_9POLY Minor capsid protein OS=Polyomavirus sp. OX=36362 PE=3 SV=1
MM1 pKa = 7.43 FLLLGMGALLAVFAEE16 pKa = 4.8 VIDD19 pKa = 4.21 LAAATGLGIDD29 pKa = 5.06 AILSAEE35 pKa = 4.64 AITTSEE41 pKa = 4.1 ALEE44 pKa = 3.88 ALITNLVTLEE54 pKa = 4.19 GFSEE58 pKa = 4.61 AEE60 pKa = 3.94 ALTAAGLTAQASAAFAEE77 pKa = 4.77 LGSLYY82 pKa = 10.03 PQILYY87 pKa = 10.94 ALAFDD92 pKa = 4.26 SLTVGTAVAAALAPNSFDD110 pKa = 3.7 HH111 pKa = 6.35 PTPIANLNMALVRR124 pKa = 11.84 WVPDD128 pKa = 3.46 FDD130 pKa = 4.67 FLFPGFRR137 pKa = 11.84 HH138 pKa = 6.31 LARR141 pKa = 11.84 FINYY145 pKa = 9.0 IDD147 pKa = 3.73 PTNWAPDD154 pKa = 3.44 LYY156 pKa = 9.09 EE157 pKa = 3.89 TLGRR161 pKa = 11.84 YY162 pKa = 7.29 VWEE165 pKa = 4.31 GLQRR169 pKa = 11.84 RR170 pKa = 11.84 GEE172 pKa = 4.08 RR173 pKa = 11.84 FIDD176 pKa = 3.68 EE177 pKa = 4.02 QIGNIEE183 pKa = 4.11 HH184 pKa = 6.57 VGRR187 pKa = 11.84 EE188 pKa = 3.72 AAIRR192 pKa = 11.84 TVEE195 pKa = 4.17 TFTEE199 pKa = 3.83 ALARR203 pKa = 11.84 YY204 pKa = 9.03 FEE206 pKa = 4.13 NARR209 pKa = 11.84 WAVSYY214 pKa = 10.18 IPHH217 pKa = 7.11 HH218 pKa = 6.8 IYY220 pKa = 10.65 SGLEE224 pKa = 3.56 KK225 pKa = 10.48 YY226 pKa = 7.88 YY227 pKa = 10.14 TQLPPLNPIQIRR239 pKa = 11.84 DD240 pKa = 3.4 VSRR243 pKa = 11.84 RR244 pKa = 11.84 TGLPIPDD251 pKa = 3.35 RR252 pKa = 11.84 TVGPNGEE259 pKa = 4.34 NVKK262 pKa = 10.17 SAQYY266 pKa = 10.21 VEE268 pKa = 5.1 KK269 pKa = 10.43 PEE271 pKa = 4.33 SPGGAMQRR279 pKa = 11.84 STPDD283 pKa = 2.44 WMLPLILGLYY293 pKa = 10.19 GDD295 pKa = 5.07 VSPSWGEE302 pKa = 3.75 TLQDD306 pKa = 4.88 LEE308 pKa = 4.54 NQDD311 pKa = 3.91 GPLKK315 pKa = 10.35 KK316 pKa = 9.93 RR317 pKa = 11.84 KK318 pKa = 8.28 KK319 pKa = 7.7 THH321 pKa = 5.25
Molecular weight: 35.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.816
IPC2_protein 4.851
IPC_protein 4.774
Toseland 4.66
ProMoST 4.851
Dawson 4.724
Bjellqvist 4.863
Wikipedia 4.571
Rodwell 4.635
Grimsley 4.571
Solomon 4.724
Lehninger 4.673
Nozaki 4.838
DTASelect 4.965
Thurlkill 4.647
EMBOSS 4.596
Sillero 4.902
Patrickios 3.897
IPC_peptide 4.736
IPC2_peptide 4.902
IPC2.peptide.svr19 4.869
Protein with the highest isoelectric point:
>tr|A0A1D8QQF4|A0A1D8QQF4_9POLY Minor capsid protein OS=Polyomavirus sp. OX=36362 PE=3 SV=1
MM1 pKa = 7.66 DD2 pKa = 4.87 HH3 pKa = 7.31 ALTRR7 pKa = 11.84 EE8 pKa = 4.04 EE9 pKa = 4.51 SVKK12 pKa = 10.73 LMQLLKK18 pKa = 10.99 LPMEE22 pKa = 4.41 QYY24 pKa = 11.84 GNFPLMRR31 pKa = 11.84 RR32 pKa = 11.84 AFLEE36 pKa = 3.98 QCKK39 pKa = 10.09 VLHH42 pKa = 6.76 PDD44 pKa = 2.8 KK45 pKa = 11.29 GGNAEE50 pKa = 4.2 LAKK53 pKa = 10.44 EE54 pKa = 4.26 LISLYY59 pKa = 10.42 RR60 pKa = 11.84 KK61 pKa = 10.03 LEE63 pKa = 3.91 AVIPPLNPNDD73 pKa = 3.78 SFTTEE78 pKa = 3.77 QVGVSDD84 pKa = 3.96 SFVFYY89 pKa = 11.01 LRR91 pKa = 11.84 DD92 pKa = 3.14 WSSCLKK98 pKa = 9.28 GTEE101 pKa = 3.73 VCICLFCLLRR111 pKa = 11.84 DD112 pKa = 3.36 SHH114 pKa = 6.52 RR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 GSKK120 pKa = 8.99 PKK122 pKa = 10.03 CWGSCFCFEE131 pKa = 5.91 CYY133 pKa = 9.67 IAWFGLEE140 pKa = 3.75 YY141 pKa = 10.01 TYY143 pKa = 10.64 FIAQSWQAIIAKK155 pKa = 10.2 LPFSTLNII163 pKa = 3.97
Molecular weight: 18.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.96
IPC2_protein 7.073
IPC_protein 7.029
Toseland 6.59
ProMoST 7.571
Dawson 7.688
Bjellqvist 8.053
Wikipedia 7.6
Rodwell 7.702
Grimsley 6.605
Solomon 7.761
Lehninger 7.775
Nozaki 8.404
DTASelect 7.834
Thurlkill 7.892
EMBOSS 7.922
Sillero 8.244
Patrickios 3.783
IPC_peptide 7.746
IPC2_peptide 7.497
IPC2.peptide.svr19 7.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1497
163
654
374.3
42.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.279 ± 1.714
2.138 ± 0.76
5.411 ± 0.565
7.148 ± 0.477
5.01 ± 0.502
6.279 ± 0.815
1.937 ± 0.203
5.411 ± 0.185
5.611 ± 1.307
10.955 ± 0.76
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.204 ± 0.231
5.478 ± 0.838
5.678 ± 0.799
3.741 ± 0.287
4.876 ± 0.247
5.611 ± 0.445
5.478 ± 0.679
5.812 ± 0.625
1.269 ± 0.33
3.674 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here