Trypanosoma vivax (strain Y486)
Average proteome isoelectric point is 7.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3778 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9WTW1|F9WTW1_TRYVY Uncharacterized protein OS=Trypanosoma vivax (strain Y486) OX=1055687 GN=TvY486_0039010 PE=4 SV=1
MM1 pKa = 7.92 LDD3 pKa = 3.98 LSHH6 pKa = 6.77 CTGITDD12 pKa = 3.97 VSPLTTLIEE21 pKa = 4.43 LKK23 pKa = 10.76 EE24 pKa = 4.19 LDD26 pKa = 5.02 LNDD29 pKa = 3.59 CTGITDD35 pKa = 3.8 VSPLSTLIRR44 pKa = 11.84 LEE46 pKa = 3.92 KK47 pKa = 10.87 LNLSDD52 pKa = 3.8 CTGITDD58 pKa = 3.92 VSPLSTLIRR67 pKa = 11.84 LEE69 pKa = 3.79 KK70 pKa = 10.79 LNLSGCTGITDD81 pKa = 3.76 VSPLTTLIEE90 pKa = 4.43 LKK92 pKa = 10.76 EE93 pKa = 4.19 LDD95 pKa = 4.98 LNDD98 pKa = 3.6 CTRR101 pKa = 11.84 ITDD104 pKa = 3.65 VSPLSTLIRR113 pKa = 11.84 LEE115 pKa = 3.98 KK116 pKa = 10.62 LCLSGCTGITDD127 pKa = 3.76 VSPLTTLIEE136 pKa = 4.21 LKK138 pKa = 10.07 EE139 pKa = 4.15 LCLSGCTGITDD150 pKa = 3.76 VSPLTTLIEE159 pKa = 4.21 LKK161 pKa = 10.7 EE162 pKa = 4.26 LGLSGCTGITDD173 pKa = 3.76 VSPLTTLIEE182 pKa = 4.21 LKK184 pKa = 10.7 EE185 pKa = 4.26 LGLSGCTGITDD196 pKa = 3.76 VSPLTTLIRR205 pKa = 11.84 LKK207 pKa = 10.53 VLYY210 pKa = 10.59 LIGCTGITDD219 pKa = 3.8 VSPLTTLIEE228 pKa = 4.24 LKK230 pKa = 10.72 EE231 pKa = 4.2 LDD233 pKa = 3.97 LHH235 pKa = 6.69 DD236 pKa = 4.19 CTGITDD242 pKa = 3.87 VSPLTTLIEE251 pKa = 4.21 LKK253 pKa = 10.7 EE254 pKa = 4.26 LGLSGCTGITDD265 pKa = 3.76 VSPLTTLIRR274 pKa = 11.84 LEE276 pKa = 3.81 VLYY279 pKa = 10.91 LIGCTGITDD288 pKa = 3.8 VSPLTTLIEE297 pKa = 4.24 LKK299 pKa = 10.72 EE300 pKa = 4.2 LDD302 pKa = 3.97 LHH304 pKa = 6.69 DD305 pKa = 4.19 CTGITDD311 pKa = 3.87 VSPLTTLIEE320 pKa = 4.29 LKK322 pKa = 10.57 EE323 pKa = 3.85 LALYY327 pKa = 10.4 GCTRR331 pKa = 11.84 ITDD334 pKa = 3.77 VSPLSALIRR343 pKa = 11.84 LEE345 pKa = 3.9 KK346 pKa = 10.7 LCLSGCTGITDD357 pKa = 3.76 VSPLTTLIRR366 pKa = 11.84 LEE368 pKa = 3.81 VLYY371 pKa = 10.91 LIGCTGITDD380 pKa = 3.8 VSPLTTLIEE389 pKa = 4.21 LKK391 pKa = 10.7 EE392 pKa = 4.26 LGLSGCTGITDD403 pKa = 3.76 VSPLTTLIEE412 pKa = 4.21 LKK414 pKa = 10.7 EE415 pKa = 4.26 LGLSGCTGITDD426 pKa = 3.76 VSPLTTLIRR435 pKa = 11.84 LEE437 pKa = 3.81 VLYY440 pKa = 10.91 LIGCTGITDD449 pKa = 3.8 VSPLTTLIEE458 pKa = 4.24 LKK460 pKa = 10.72 EE461 pKa = 4.2 LDD463 pKa = 3.97 LHH465 pKa = 6.66 DD466 pKa = 4.18 CTGISDD472 pKa = 3.94 VSPLPMMIRR481 pKa = 11.84 PEE483 pKa = 4.06 VLYY486 pKa = 10.03 MIGCTGISDD495 pKa = 3.94 VSPLTTMIEE504 pKa = 4.33 FKK506 pKa = 10.55 GAEE509 pKa = 4.12 YY510 pKa = 10.32 EE511 pKa = 4.1
Molecular weight: 54.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 4.113
IPC_protein 4.101
Toseland 3.91
ProMoST 4.215
Dawson 4.062
Bjellqvist 4.228
Wikipedia 3.961
Rodwell 3.935
Grimsley 3.821
Solomon 4.062
Lehninger 4.012
Nozaki 4.164
DTASelect 4.368
Thurlkill 3.935
EMBOSS 3.973
Sillero 4.215
Patrickios 1.214
IPC_peptide 4.062
IPC2_peptide 4.202
IPC2.peptide.svr19 4.09
Protein with the highest isoelectric point:
>tr|F9WKS7|F9WKS7_TRYVY Uncharacterized protein OS=Trypanosoma vivax (strain Y486) OX=1055687 GN=TvY486_0007440 PE=4 SV=1
MM1 pKa = 7.69 RR2 pKa = 11.84 GKK4 pKa = 9.72 HH5 pKa = 4.17 VATRR9 pKa = 11.84 SHH11 pKa = 5.44 CHH13 pKa = 5.85 NYY15 pKa = 9.83 RR16 pKa = 11.84 SAGQTQDD23 pKa = 3.12 AQHH26 pKa = 6.63 PLQRR30 pKa = 11.84 RR31 pKa = 11.84 AACCVSEE38 pKa = 4.56 HH39 pKa = 6.15 AWTVHH44 pKa = 5.06 GASTRR49 pKa = 11.84 TAASSACPGKK59 pKa = 8.38 TLPIVHH65 pKa = 7.17 AEE67 pKa = 3.79 PTRR70 pKa = 11.84 APRR73 pKa = 11.84 CCHH76 pKa = 7.22 LLCCPDD82 pKa = 3.34 ASGMRR87 pKa = 11.84 NADD90 pKa = 3.53 TQRR93 pKa = 11.84 NDD95 pKa = 3.25 HH96 pKa = 6.74 AVDD99 pKa = 3.48 GCIFQKK105 pKa = 7.89 EE106 pKa = 4.26 TRR108 pKa = 11.84 QPSNASAPSLAHH120 pKa = 5.75 IVMVTLHH127 pKa = 6.89 RR128 pKa = 11.84 SCATQQTCRR137 pKa = 11.84 SMRR140 pKa = 11.84 APLPGHH146 pKa = 6.8 LPSSGAHH153 pKa = 4.07 SVQRR157 pKa = 11.84 RR158 pKa = 11.84 EE159 pKa = 3.78 TRR161 pKa = 11.84 AVAMHH166 pKa = 5.65 STISTQHH173 pKa = 6.27 KK174 pKa = 10.38 GEE176 pKa = 4.26 NNTKK180 pKa = 9.58 KK181 pKa = 10.62 ARR183 pKa = 11.84 VRR185 pKa = 11.84 HH186 pKa = 5.76 LPASQSTVGQPTHH199 pKa = 5.87 TRR201 pKa = 11.84 TITQMCPAVTVGHH214 pKa = 6.92 HH215 pKa = 6.79 APPAPPPSTYY225 pKa = 9.78 DD226 pKa = 3.26 TAIQRR231 pKa = 11.84 PAALAYY237 pKa = 10.3 SSQLRR242 pKa = 11.84 TALHH246 pKa = 6.23 EE247 pKa = 4.25 ARR249 pKa = 11.84 APLRR253 pKa = 11.84 LPLFKK258 pKa = 10.29 QQHH261 pKa = 6.9 AYY263 pKa = 7.73 TQLTAIVTRR272 pKa = 11.84 QRR274 pKa = 11.84 QLPVSLASDD283 pKa = 3.81 NLSKK287 pKa = 10.97 QPGKK291 pKa = 10.4 SPRR294 pKa = 3.4
Molecular weight: 31.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.359
IPC2_protein 9.516
IPC_protein 10.277
Toseland 10.613
ProMoST 10.482
Dawson 10.701
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.847
Grimsley 10.745
Solomon 10.833
Lehninger 10.804
Nozaki 10.672
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.023
Sillero 10.657
Patrickios 10.57
IPC_peptide 10.833
IPC2_peptide 9.94
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3778
0
3778
1476553
8
4061
390.8
42.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.307 ± 0.092
2.516 ± 0.024
4.517 ± 0.026
6.407 ± 0.041
2.592 ± 0.026
6.928 ± 0.034
2.723 ± 0.023
3.04 ± 0.023
5.456 ± 0.043
8.357 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.985 ± 0.014
3.629 ± 0.026
4.004 ± 0.043
3.976 ± 0.03
7.899 ± 0.055
7.426 ± 0.043
6.856 ± 0.049
6.638 ± 0.044
1.269 ± 0.013
1.473 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here