Halostagnicola sp. A56

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halostagnicola; unclassified Halostagnicola

Average proteome isoelectric point is 4.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8FB39|A0A0J8FB39_9EURY Uncharacterized protein OS=Halostagnicola sp. A56 OX=1495067 GN=EL22_28890 PE=4 SV=1
MM1 pKa = 7.68TDD3 pKa = 4.7AIDD6 pKa = 4.56DD7 pKa = 4.11DD8 pKa = 5.06LYY10 pKa = 11.55QRR12 pKa = 11.84TKK14 pKa = 11.36ALLEE18 pKa = 4.72PGDD21 pKa = 3.7IDD23 pKa = 5.03LNATVVHH30 pKa = 6.51TDD32 pKa = 2.83YY33 pKa = 11.47DD34 pKa = 3.76GQEE37 pKa = 4.27DD38 pKa = 4.52VKK40 pKa = 10.96MMQATIDD47 pKa = 3.5VGDD50 pKa = 5.07IIAEE54 pKa = 4.29HH55 pKa = 6.8AGHH58 pKa = 7.17DD59 pKa = 3.72PKK61 pKa = 11.53DD62 pKa = 3.73CFVYY66 pKa = 10.6SGNDD70 pKa = 3.25DD71 pKa = 3.27TDD73 pKa = 3.74YY74 pKa = 11.51SSNQHH79 pKa = 5.99QGLTLEE85 pKa = 4.79DD86 pKa = 4.41EE87 pKa = 4.47EE88 pKa = 5.6FVWEE92 pKa = 4.29CQQLLRR98 pKa = 11.84NGSFDD103 pKa = 3.02IVIYY107 pKa = 10.42YY108 pKa = 7.69EE109 pKa = 4.17ASADD113 pKa = 3.65HH114 pKa = 6.5EE115 pKa = 5.49AILSDD120 pKa = 3.93VRR122 pKa = 11.84DD123 pKa = 3.58LGFDD127 pKa = 3.29VTGVEE132 pKa = 4.95GEE134 pKa = 4.07

Molecular weight: 14.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A063ZN68|A0A063ZN68_9EURY Uncharacterized protein OS=Halostagnicola sp. A56 OX=1495067 GN=EL22_21625 PE=4 SV=1
MM1 pKa = 7.68TSTDD5 pKa = 3.51DD6 pKa = 3.78PSRR9 pKa = 11.84ARR11 pKa = 11.84PPLPLRR17 pKa = 11.84TRR19 pKa = 11.84ARR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84VLPTLHH29 pKa = 7.3RR30 pKa = 11.84LKK32 pKa = 10.7QPLGGFAQCRR42 pKa = 11.84QHH44 pKa = 5.82PAEE47 pKa = 4.16YY48 pKa = 10.12VGTVQRR54 pKa = 11.84SLEE57 pKa = 4.07EE58 pKa = 3.67FRR60 pKa = 11.84ADD62 pKa = 3.56LEE64 pKa = 4.41AMSFSPEE71 pKa = 4.15PIASLKK77 pKa = 7.63VHH79 pKa = 6.69RR80 pKa = 11.84DD81 pKa = 3.1GRR83 pKa = 11.84LSAGSWVRR91 pKa = 11.84RR92 pKa = 11.84PSPLSTWQLHH102 pKa = 4.52VALFRR107 pKa = 11.84TDD109 pKa = 3.77DD110 pKa = 3.39RR111 pKa = 11.84SLEE114 pKa = 4.2VFAHH118 pKa = 7.14RR119 pKa = 11.84EE120 pKa = 4.06YY121 pKa = 11.1SWIRR125 pKa = 11.84HH126 pKa = 5.33PYY128 pKa = 8.21KK129 pKa = 10.56HH130 pKa = 5.36YY131 pKa = 9.94TGEE134 pKa = 4.08GWDD137 pKa = 3.63TKK139 pKa = 11.08SGVDD143 pKa = 4.31RR144 pKa = 11.84MRR146 pKa = 11.84ALLGRR151 pKa = 11.84HH152 pKa = 4.82GVSFSVEE159 pKa = 3.65

Molecular weight: 18.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2711

0

2711

722748

25

1713

266.6

29.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.15 ± 0.056

0.756 ± 0.015

8.34 ± 0.06

9.253 ± 0.066

3.313 ± 0.034

8.179 ± 0.055

1.952 ± 0.023

4.679 ± 0.032

1.91 ± 0.029

8.781 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.791 ± 0.019

2.51 ± 0.025

4.443 ± 0.033

2.592 ± 0.027

6.367 ± 0.06

6.107 ± 0.038

6.516 ± 0.035

8.59 ± 0.046

1.078 ± 0.019

2.693 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski