Neisseria arctica
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0J1C113|A0A0J1C113_9NEIS Polyamine ABC transporter substrate-binding protein (Fragment) OS=Neisseria arctica OX=1470200 GN=PL75_10900 PE=4 SV=1
MM1 pKa = 7.15 NADD4 pKa = 3.07 EE5 pKa = 4.76 HH6 pKa = 7.21 GYY8 pKa = 10.27 YY9 pKa = 10.26 CGSAEE14 pKa = 4.6 KK15 pKa = 9.67 DD16 pKa = 3.7 TITGTDD22 pKa = 3.59 GEE24 pKa = 4.63 DD25 pKa = 3.3 VIFGAGGPVVIYY37 pKa = 10.61 GGDD40 pKa = 3.66 GNDD43 pKa = 4.14 TIVGGPGGDD52 pKa = 3.49 SLDD55 pKa = 3.68 GGKK58 pKa = 10.75 GKK60 pKa = 10.9 GYY62 pKa = 9.85 LQSSDD67 pKa = 3.05 GKK69 pKa = 10.8
Molecular weight: 6.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.75
IPC2_protein 3.923
IPC_protein 3.859
Toseland 3.643
ProMoST 4.05
Dawson 3.872
Bjellqvist 4.037
Wikipedia 3.846
Rodwell 3.694
Grimsley 3.554
Solomon 3.846
Lehninger 3.808
Nozaki 3.999
DTASelect 4.266
Thurlkill 3.719
EMBOSS 3.859
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.846
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A0J0YR30|A0A0J0YR30_9NEIS Acetyltransferase component of pyruvate dehydrogenase complex OS=Neisseria arctica OX=1470200 GN=PL75_07670 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.06 QPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.43 RR14 pKa = 11.84 THH16 pKa = 5.89 GFLVRR21 pKa = 11.84 SKK23 pKa = 9.38 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.87 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2157
0
2157
629626
30
3371
291.9
32.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.166 ± 0.074
0.995 ± 0.018
5.034 ± 0.038
6.057 ± 0.06
3.93 ± 0.042
7.451 ± 0.062
2.192 ± 0.029
6.017 ± 0.038
5.059 ± 0.049
10.31 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.589 ± 0.027
4.229 ± 0.058
4.237 ± 0.04
4.364 ± 0.051
5.172 ± 0.047
5.723 ± 0.05
5.242 ± 0.058
6.934 ± 0.059
1.3 ± 0.025
3.001 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here