Desulfovibrio gracilis DSM 16080

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfovibrionales; Desulfovibrionaceae; Desulfovibrio; Desulfovibrio gracilis

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2871 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4W552|A0A1T4W552_9DELT Glutamine cyclotransferase OS=Desulfovibrio gracilis DSM 16080 OX=1121449 GN=SAMN02745704_00335 PE=4 SV=1
MM1 pKa = 7.35TEE3 pKa = 3.88STFNEE8 pKa = 3.54INEE11 pKa = 5.15FVTQWNDD18 pKa = 2.59NGNRR22 pKa = 11.84CKK24 pKa = 10.97DD25 pKa = 3.15CFLRR29 pKa = 11.84LKK31 pKa = 7.99QHH33 pKa = 6.5CEE35 pKa = 3.59GMDD38 pKa = 4.46GIRR41 pKa = 11.84LEE43 pKa = 4.47WIARR47 pKa = 11.84PGITYY52 pKa = 9.67SLRR55 pKa = 11.84ATHH58 pKa = 6.14SQQADD63 pKa = 3.1SDD65 pKa = 4.19RR66 pKa = 11.84NLFAMIDD73 pKa = 3.97IIDD76 pKa = 5.29DD77 pKa = 4.21DD78 pKa = 5.04PSDD81 pKa = 3.35RR82 pKa = 11.84WLSVCFYY89 pKa = 10.77NDD91 pKa = 3.25MVSDD95 pKa = 4.11PDD97 pKa = 3.43EE98 pKa = 6.15AGDD101 pKa = 3.88YY102 pKa = 10.77VPEE105 pKa = 4.52GLLGQDD111 pKa = 3.48ALCFDD116 pKa = 4.16VEE118 pKa = 4.38SWDD121 pKa = 4.36DD122 pKa = 3.38GHH124 pKa = 8.45LGYY127 pKa = 10.5VEE129 pKa = 4.49SRR131 pKa = 11.84LSEE134 pKa = 4.35ACSCAAGGSDD144 pKa = 3.72EE145 pKa = 4.16

Molecular weight: 16.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4W3G5|A0A1T4W3G5_9DELT Hydrogenase expression/formation protein HypC OS=Desulfovibrio gracilis DSM 16080 OX=1121449 GN=SAMN02745704_00188 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 9.6RR3 pKa = 11.84TFQPRR8 pKa = 11.84NLKK11 pKa = 10.19RR12 pKa = 11.84KK13 pKa = 7.32RR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84SRR23 pKa = 11.84TKK25 pKa = 10.25NGRR28 pKa = 11.84AVLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 8.55RR41 pKa = 11.84LSVV44 pKa = 3.2

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2871

0

2871

934146

39

4681

325.4

35.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.593 ± 0.056

1.352 ± 0.022

5.652 ± 0.043

6.732 ± 0.046

3.914 ± 0.031

8.072 ± 0.042

2.261 ± 0.021

4.576 ± 0.037

3.855 ± 0.044

10.979 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.74 ± 0.023

2.94 ± 0.031

4.973 ± 0.031

3.708 ± 0.027

7.037 ± 0.054

5.418 ± 0.033

4.975 ± 0.038

7.481 ± 0.036

1.247 ± 0.02

2.494 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski