Oleiphilus messinensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oleiphilaceae; Oleiphilus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1Y0I9X1|A0A1Y0I9X1_9GAMM Uncharacterized protein OS=Oleiphilus messinensis OX=141451 GN=OLMES_2153 PE=4 SV=1
MM1 pKa = 7.55RR2 pKa = 11.84PSRR5 pKa = 11.84SLLVDD10 pKa = 5.0PISGPSHH17 pKa = 6.29GKK19 pKa = 10.09ALLSDD24 pKa = 3.68TLLRR28 pKa = 11.84AQNALQDD35 pKa = 4.21FVAKK39 pKa = 10.48DD40 pKa = 3.54VFDD43 pKa = 3.82TKK45 pKa = 11.07VFTVAFGEE53 pKa = 4.23NYY55 pKa = 10.17DD56 pKa = 4.14AIIFEE61 pKa = 4.45SLIDD65 pKa = 3.43QYY67 pKa = 11.95RR68 pKa = 11.84NGNFSSLPSIEE79 pKa = 4.27LRR81 pKa = 11.84SGLEE85 pKa = 3.73LQGADD90 pKa = 3.15GVYY93 pKa = 10.09VEE95 pKa = 4.85SLNAIFISDD104 pKa = 3.91TFYY107 pKa = 11.88GDD109 pKa = 4.88ANDD112 pKa = 4.07TQQVAVVLEE121 pKa = 4.64EE122 pKa = 3.9IGHH125 pKa = 7.02AIDD128 pKa = 4.74ALINKK133 pKa = 9.21SDD135 pKa = 3.61SKK137 pKa = 11.22GDD139 pKa = 3.42EE140 pKa = 3.59GRR142 pKa = 11.84IFAKK146 pKa = 10.45LVLGQEE152 pKa = 4.14MSDD155 pKa = 3.33EE156 pKa = 4.57VVAEE160 pKa = 3.99LHH162 pKa = 7.16AEE164 pKa = 4.17DD165 pKa = 5.9DD166 pKa = 4.31SLTLTVDD173 pKa = 3.51GQTVLAEE180 pKa = 4.35ASTKK184 pKa = 10.18VWDD187 pKa = 4.01SGDD190 pKa = 3.01IGGPYY195 pKa = 8.44ATTWKK200 pKa = 10.48YY201 pKa = 10.24ILKK204 pKa = 9.39TIDD207 pKa = 3.31TPYY210 pKa = 11.17SMSSTYY216 pKa = 10.37FEE218 pKa = 4.21NTLKK222 pKa = 10.44HH223 pKa = 6.19DD224 pKa = 4.06RR225 pKa = 11.84TWSDD229 pKa = 3.57FIGNKK234 pKa = 6.94TKK236 pKa = 10.29YY237 pKa = 8.24YY238 pKa = 10.23EE239 pKa = 4.18YY240 pKa = 10.35YY241 pKa = 10.97GEE243 pKa = 4.38VNGTSGDD250 pKa = 4.09DD251 pKa = 3.73IIIGATDD258 pKa = 3.53TKK260 pKa = 11.0KK261 pKa = 10.44KK262 pKa = 10.07WEE264 pKa = 4.91DD265 pKa = 3.58GNNDD269 pKa = 2.43IYY271 pKa = 11.13TWLRR275 pKa = 11.84AQEE278 pKa = 3.88EE279 pKa = 4.9TINGGSGDD287 pKa = 4.24DD288 pKa = 4.42YY289 pKa = 11.46IDD291 pKa = 4.41GGDD294 pKa = 4.08GSDD297 pKa = 4.32TINGGAGNDD306 pKa = 4.09LIFTGDD312 pKa = 3.85LSDD315 pKa = 5.17GSDD318 pKa = 3.7DD319 pKa = 4.69DD320 pKa = 5.4VITGGSGADD329 pKa = 3.2TFYY332 pKa = 10.74TGIITHH338 pKa = 6.86PTTTGYY344 pKa = 11.04DD345 pKa = 3.13SDD347 pKa = 4.85FNFGNLALSMAGDD360 pKa = 3.84FTDD363 pKa = 5.53LLFTLSGTKK372 pKa = 7.99ITKK375 pKa = 9.89EE376 pKa = 3.91IVPMAFDD383 pKa = 3.31VLKK386 pKa = 10.9AVLNDD391 pKa = 3.23EE392 pKa = 4.41SGNYY396 pKa = 7.0QTVEE400 pKa = 4.13DD401 pKa = 4.18ATAAHH406 pKa = 6.72YY407 pKa = 7.89ATITDD412 pKa = 4.22FNPRR416 pKa = 11.84EE417 pKa = 4.08DD418 pKa = 3.75VFIVPLASTGNINIDD433 pKa = 2.98ITTDD437 pKa = 4.19GIGNSAFALDD447 pKa = 4.01YY448 pKa = 11.48VNGNGRR454 pKa = 11.84LAKK457 pKa = 10.15VYY459 pKa = 9.61YY460 pKa = 9.95DD461 pKa = 3.74SSISDD466 pKa = 3.6SAQNFYY472 pKa = 11.1GKK474 pKa = 10.58ALLDD478 pKa = 3.39SALIIEE484 pKa = 4.82NDD486 pKa = 3.1AVTYY490 pKa = 10.74GGDD493 pKa = 3.43DD494 pKa = 3.34SGSIFSSSDD503 pKa = 3.05LYY505 pKa = 11.64ADD507 pKa = 4.73LSDD510 pKa = 4.84DD511 pKa = 3.91VKK513 pKa = 11.47ASLGNLSGRR522 pKa = 11.84YY523 pKa = 8.32MILGAFSGSYY533 pKa = 10.61YY534 pKa = 10.55EE535 pKa = 5.2DD536 pKa = 4.49LYY538 pKa = 11.6GNSDD542 pKa = 3.21EE543 pKa = 5.85GMYY546 pKa = 8.33GTNYY550 pKa = 10.51GDD552 pKa = 3.65VLYY555 pKa = 10.09GYY557 pKa = 9.92QKK559 pKa = 9.42EE560 pKa = 4.81TTDD563 pKa = 2.87SWSPQNDD570 pKa = 3.55GNDD573 pKa = 3.2YY574 pKa = 10.47FYY576 pKa = 10.81GYY578 pKa = 9.17TGDD581 pKa = 3.68DD582 pKa = 3.18VFYY585 pKa = 10.95GGGGINYY592 pKa = 8.53FFGGEE597 pKa = 3.84SAYY600 pKa = 11.29NNSGYY605 pKa = 10.54NEE607 pKa = 4.18SADD610 pKa = 3.65NGSDD614 pKa = 3.19TVSYY618 pKa = 11.15EE619 pKa = 4.12DD620 pKa = 3.96ANSGAGVNIDD630 pKa = 3.98LSVTATDD637 pKa = 3.53QNGTYY642 pKa = 9.89TLVSANGFISDD653 pKa = 3.83QNVEE657 pKa = 4.26GVDD660 pKa = 3.11RR661 pKa = 11.84LYY663 pKa = 10.98SIEE666 pKa = 4.5NIIGTEE672 pKa = 3.86FADD675 pKa = 4.44TITGNSGDD683 pKa = 3.87NVLGGSGGNDD693 pKa = 2.74ILKK696 pKa = 10.66GEE698 pKa = 4.23SGNDD702 pKa = 2.76IFYY705 pKa = 10.91GGSGSNEE712 pKa = 3.17IWGGSGTDD720 pKa = 3.08TVDD723 pKa = 3.29YY724 pKa = 10.84SRR726 pKa = 11.84STSGVSGSTVNWSKK740 pKa = 9.22ITHH743 pKa = 5.89SEE745 pKa = 4.85GIDD748 pKa = 3.22QFKK751 pKa = 11.2NNDD754 pKa = 3.35GGGVEE759 pKa = 4.32IVIGSDD765 pKa = 3.27YY766 pKa = 11.32DD767 pKa = 3.74DD768 pKa = 5.17TITGHH773 pKa = 5.69WVANTLYY780 pKa = 10.51GGAGNDD786 pKa = 3.26ILNGGGGNYY795 pKa = 10.3ADD797 pKa = 4.35TLDD800 pKa = 4.49GGSGSDD806 pKa = 3.39TLRR809 pKa = 11.84GANGNDD815 pKa = 3.35TLIGGGGQDD824 pKa = 2.82RR825 pKa = 11.84FEE827 pKa = 4.74FDD829 pKa = 3.97SDD831 pKa = 4.38DD832 pKa = 3.87GTDD835 pKa = 3.35TITDD839 pKa = 3.55YY840 pKa = 11.55SSNSDD845 pKa = 3.45TLSFDD850 pKa = 3.16GDD852 pKa = 4.16VVVTSYY858 pKa = 11.81SDD860 pKa = 3.78GSDD863 pKa = 3.43TYY865 pKa = 11.64VSAGDD870 pKa = 3.63TTVKK874 pKa = 10.58LVGVSNGDD882 pKa = 3.21IASGFSYY889 pKa = 10.82QSDD892 pKa = 3.79TNTTEE897 pKa = 4.11NGPSGNTVICTYY909 pKa = 7.25MHH911 pKa = 7.38EE912 pKa = 4.22IGEE915 pKa = 4.35ISDD918 pKa = 5.31EE919 pKa = 4.34VYY921 pKa = 10.52QWDD924 pKa = 3.55QYY926 pKa = 10.94YY927 pKa = 10.89GRR929 pKa = 11.84EE930 pKa = 4.08FLGKK934 pKa = 7.66TVLRR938 pKa = 11.84GYY940 pKa = 9.9HH941 pKa = 5.18YY942 pKa = 9.85WAIPFVRR949 pKa = 11.84AMRR952 pKa = 11.84RR953 pKa = 11.84NPRR956 pKa = 11.84LTQVMIPLAKK966 pKa = 10.33AWTQEE971 pKa = 3.8MAHH974 pKa = 6.55RR975 pKa = 11.84CDD977 pKa = 4.19PDD979 pKa = 3.3NHH981 pKa = 5.06EE982 pKa = 4.41HH983 pKa = 6.44NRR985 pKa = 11.84LGSLALAVGVPPSRR999 pKa = 11.84IIGSVLEE1006 pKa = 3.98ANYY1009 pKa = 10.32RR1010 pKa = 11.84RR1011 pKa = 11.84LSALRR1016 pKa = 11.84KK1017 pKa = 9.64SAVIDD1022 pKa = 3.59SS1023 pKa = 3.93

Molecular weight:
109.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1Y0IGN0|A0A1Y0IGN0_9GAMM Paraquat-inducible protein B OS=Oleiphilus messinensis OX=141451 GN=OLMES_5299 PE=4 SV=1
MM1 pKa = 7.94AEE3 pKa = 4.12FLSIGAAAFLLGVAVSTLRR22 pKa = 11.84CWEE25 pKa = 3.88KK26 pKa = 10.5EE27 pKa = 3.8SRR29 pKa = 11.84FFSDD33 pKa = 3.57FRR35 pKa = 11.84TPGGHH40 pKa = 6.3RR41 pKa = 11.84RR42 pKa = 11.84YY43 pKa = 10.62ALDD46 pKa = 4.28KK47 pKa = 10.97LLAFCGQSTANEE59 pKa = 3.89QRR61 pKa = 11.84RR62 pKa = 11.84TICYY66 pKa = 10.24ARR68 pKa = 11.84VSSHH72 pKa = 6.2DD73 pKa = 3.59QKK75 pKa = 11.66KK76 pKa = 10.55DD77 pKa = 3.21LQTQIARR84 pKa = 11.84LYY86 pKa = 9.97GSRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84KK92 pKa = 7.75NQRR95 pKa = 11.84AIAA98 pKa = 3.94

Molecular weight:
11.2 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5456

0

5456

1829888

19

7866

335.4

37.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.581 ± 0.035

1.043 ± 0.012

5.663 ± 0.031

6.396 ± 0.033

4.108 ± 0.023

6.811 ± 0.04

2.305 ± 0.018

6.169 ± 0.024

4.736 ± 0.032

10.519 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.314 ± 0.018

4.087 ± 0.024

4.238 ± 0.023

4.609 ± 0.023

5.284 ± 0.026

6.734 ± 0.028

5.382 ± 0.03

6.795 ± 0.032

1.253 ± 0.013

2.974 ± 0.018

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski