Oleiphilus messinensis
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5456 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0I9X1|A0A1Y0I9X1_9GAMM Uncharacterized protein OS=Oleiphilus messinensis OX=141451 GN=OLMES_2153 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 PSRR5 pKa = 11.84 SLLVDD10 pKa = 5.0 PISGPSHH17 pKa = 6.29 GKK19 pKa = 10.09 ALLSDD24 pKa = 3.68 TLLRR28 pKa = 11.84 AQNALQDD35 pKa = 4.21 FVAKK39 pKa = 10.48 DD40 pKa = 3.54 VFDD43 pKa = 3.82 TKK45 pKa = 11.07 VFTVAFGEE53 pKa = 4.23 NYY55 pKa = 10.17 DD56 pKa = 4.14 AIIFEE61 pKa = 4.45 SLIDD65 pKa = 3.43 QYY67 pKa = 11.95 RR68 pKa = 11.84 NGNFSSLPSIEE79 pKa = 4.27 LRR81 pKa = 11.84 SGLEE85 pKa = 3.73 LQGADD90 pKa = 3.15 GVYY93 pKa = 10.09 VEE95 pKa = 4.85 SLNAIFISDD104 pKa = 3.91 TFYY107 pKa = 11.88 GDD109 pKa = 4.88 ANDD112 pKa = 4.07 TQQVAVVLEE121 pKa = 4.64 EE122 pKa = 3.9 IGHH125 pKa = 7.02 AIDD128 pKa = 4.74 ALINKK133 pKa = 9.21 SDD135 pKa = 3.61 SKK137 pKa = 11.22 GDD139 pKa = 3.42 EE140 pKa = 3.59 GRR142 pKa = 11.84 IFAKK146 pKa = 10.45 LVLGQEE152 pKa = 4.14 MSDD155 pKa = 3.33 EE156 pKa = 4.57 VVAEE160 pKa = 3.99 LHH162 pKa = 7.16 AEE164 pKa = 4.17 DD165 pKa = 5.9 DD166 pKa = 4.31 SLTLTVDD173 pKa = 3.51 GQTVLAEE180 pKa = 4.35 ASTKK184 pKa = 10.18 VWDD187 pKa = 4.01 SGDD190 pKa = 3.01 IGGPYY195 pKa = 8.44 ATTWKK200 pKa = 10.48 YY201 pKa = 10.24 ILKK204 pKa = 9.39 TIDD207 pKa = 3.31 TPYY210 pKa = 11.17 SMSSTYY216 pKa = 10.37 FEE218 pKa = 4.21 NTLKK222 pKa = 10.44 HH223 pKa = 6.19 DD224 pKa = 4.06 RR225 pKa = 11.84 TWSDD229 pKa = 3.57 FIGNKK234 pKa = 6.94 TKK236 pKa = 10.29 YY237 pKa = 8.24 YY238 pKa = 10.23 EE239 pKa = 4.18 YY240 pKa = 10.35 YY241 pKa = 10.97 GEE243 pKa = 4.38 VNGTSGDD250 pKa = 4.09 DD251 pKa = 3.73 IIIGATDD258 pKa = 3.53 TKK260 pKa = 11.0 KK261 pKa = 10.44 KK262 pKa = 10.07 WEE264 pKa = 4.91 DD265 pKa = 3.58 GNNDD269 pKa = 2.43 IYY271 pKa = 11.13 TWLRR275 pKa = 11.84 AQEE278 pKa = 3.88 EE279 pKa = 4.9 TINGGSGDD287 pKa = 4.24 DD288 pKa = 4.42 YY289 pKa = 11.46 IDD291 pKa = 4.41 GGDD294 pKa = 4.08 GSDD297 pKa = 4.32 TINGGAGNDD306 pKa = 4.09 LIFTGDD312 pKa = 3.85 LSDD315 pKa = 5.17 GSDD318 pKa = 3.7 DD319 pKa = 4.69 DD320 pKa = 5.4 VITGGSGADD329 pKa = 3.2 TFYY332 pKa = 10.74 TGIITHH338 pKa = 6.86 PTTTGYY344 pKa = 11.04 DD345 pKa = 3.13 SDD347 pKa = 4.85 FNFGNLALSMAGDD360 pKa = 3.84 FTDD363 pKa = 5.53 LLFTLSGTKK372 pKa = 7.99 ITKK375 pKa = 9.89 EE376 pKa = 3.91 IVPMAFDD383 pKa = 3.31 VLKK386 pKa = 10.9 AVLNDD391 pKa = 3.23 EE392 pKa = 4.41 SGNYY396 pKa = 7.0 QTVEE400 pKa = 4.13 DD401 pKa = 4.18 ATAAHH406 pKa = 6.72 YY407 pKa = 7.89 ATITDD412 pKa = 4.22 FNPRR416 pKa = 11.84 EE417 pKa = 4.08 DD418 pKa = 3.75 VFIVPLASTGNINIDD433 pKa = 2.98 ITTDD437 pKa = 4.19 GIGNSAFALDD447 pKa = 4.01 YY448 pKa = 11.48 VNGNGRR454 pKa = 11.84 LAKK457 pKa = 10.15 VYY459 pKa = 9.61 YY460 pKa = 9.95 DD461 pKa = 3.74 SSISDD466 pKa = 3.6 SAQNFYY472 pKa = 11.1 GKK474 pKa = 10.58 ALLDD478 pKa = 3.39 SALIIEE484 pKa = 4.82 NDD486 pKa = 3.1 AVTYY490 pKa = 10.74 GGDD493 pKa = 3.43 DD494 pKa = 3.34 SGSIFSSSDD503 pKa = 3.05 LYY505 pKa = 11.64 ADD507 pKa = 4.73 LSDD510 pKa = 4.84 DD511 pKa = 3.91 VKK513 pKa = 11.47 ASLGNLSGRR522 pKa = 11.84 YY523 pKa = 8.32 MILGAFSGSYY533 pKa = 10.61 YY534 pKa = 10.55 EE535 pKa = 5.2 DD536 pKa = 4.49 LYY538 pKa = 11.6 GNSDD542 pKa = 3.21 EE543 pKa = 5.85 GMYY546 pKa = 8.33 GTNYY550 pKa = 10.51 GDD552 pKa = 3.65 VLYY555 pKa = 10.09 GYY557 pKa = 9.92 QKK559 pKa = 9.42 EE560 pKa = 4.81 TTDD563 pKa = 2.87 SWSPQNDD570 pKa = 3.55 GNDD573 pKa = 3.2 YY574 pKa = 10.47 FYY576 pKa = 10.81 GYY578 pKa = 9.17 TGDD581 pKa = 3.68 DD582 pKa = 3.18 VFYY585 pKa = 10.95 GGGGINYY592 pKa = 8.53 FFGGEE597 pKa = 3.84 SAYY600 pKa = 11.29 NNSGYY605 pKa = 10.54 NEE607 pKa = 4.18 SADD610 pKa = 3.65 NGSDD614 pKa = 3.19 TVSYY618 pKa = 11.15 EE619 pKa = 4.12 DD620 pKa = 3.96 ANSGAGVNIDD630 pKa = 3.98 LSVTATDD637 pKa = 3.53 QNGTYY642 pKa = 9.89 TLVSANGFISDD653 pKa = 3.83 QNVEE657 pKa = 4.26 GVDD660 pKa = 3.11 RR661 pKa = 11.84 LYY663 pKa = 10.98 SIEE666 pKa = 4.5 NIIGTEE672 pKa = 3.86 FADD675 pKa = 4.44 TITGNSGDD683 pKa = 3.87 NVLGGSGGNDD693 pKa = 2.74 ILKK696 pKa = 10.66 GEE698 pKa = 4.23 SGNDD702 pKa = 2.76 IFYY705 pKa = 10.91 GGSGSNEE712 pKa = 3.17 IWGGSGTDD720 pKa = 3.08 TVDD723 pKa = 3.29 YY724 pKa = 10.84 SRR726 pKa = 11.84 STSGVSGSTVNWSKK740 pKa = 9.22 ITHH743 pKa = 5.89 SEE745 pKa = 4.85 GIDD748 pKa = 3.22 QFKK751 pKa = 11.2 NNDD754 pKa = 3.35 GGGVEE759 pKa = 4.32 IVIGSDD765 pKa = 3.27 YY766 pKa = 11.32 DD767 pKa = 3.74 DD768 pKa = 5.17 TITGHH773 pKa = 5.69 WVANTLYY780 pKa = 10.51 GGAGNDD786 pKa = 3.26 ILNGGGGNYY795 pKa = 10.3 ADD797 pKa = 4.35 TLDD800 pKa = 4.49 GGSGSDD806 pKa = 3.39 TLRR809 pKa = 11.84 GANGNDD815 pKa = 3.35 TLIGGGGQDD824 pKa = 2.82 RR825 pKa = 11.84 FEE827 pKa = 4.74 FDD829 pKa = 3.97 SDD831 pKa = 4.38 DD832 pKa = 3.87 GTDD835 pKa = 3.35 TITDD839 pKa = 3.55 YY840 pKa = 11.55 SSNSDD845 pKa = 3.45 TLSFDD850 pKa = 3.16 GDD852 pKa = 4.16 VVVTSYY858 pKa = 11.81 SDD860 pKa = 3.78 GSDD863 pKa = 3.43 TYY865 pKa = 11.64 VSAGDD870 pKa = 3.63 TTVKK874 pKa = 10.58 LVGVSNGDD882 pKa = 3.21 IASGFSYY889 pKa = 10.82 QSDD892 pKa = 3.79 TNTTEE897 pKa = 4.11 NGPSGNTVICTYY909 pKa = 7.25 MHH911 pKa = 7.38 EE912 pKa = 4.22 IGEE915 pKa = 4.35 ISDD918 pKa = 5.31 EE919 pKa = 4.34 VYY921 pKa = 10.52 QWDD924 pKa = 3.55 QYY926 pKa = 10.94 YY927 pKa = 10.89 GRR929 pKa = 11.84 EE930 pKa = 4.08 FLGKK934 pKa = 7.66 TVLRR938 pKa = 11.84 GYY940 pKa = 9.9 HH941 pKa = 5.18 YY942 pKa = 9.85 WAIPFVRR949 pKa = 11.84 AMRR952 pKa = 11.84 RR953 pKa = 11.84 NPRR956 pKa = 11.84 LTQVMIPLAKK966 pKa = 10.33 AWTQEE971 pKa = 3.8 MAHH974 pKa = 6.55 RR975 pKa = 11.84 CDD977 pKa = 4.19 PDD979 pKa = 3.3 NHH981 pKa = 5.06 EE982 pKa = 4.41 HH983 pKa = 6.44 NRR985 pKa = 11.84 LGSLALAVGVPPSRR999 pKa = 11.84 IIGSVLEE1006 pKa = 3.98 ANYY1009 pKa = 10.32 RR1010 pKa = 11.84 RR1011 pKa = 11.84 LSALRR1016 pKa = 11.84 KK1017 pKa = 9.64 SAVIDD1022 pKa = 3.59 SS1023 pKa = 3.93
Molecular weight: 109.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.697
IPC2_protein 3.846
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.846
Rodwell 3.719
Grimsley 3.567
Solomon 3.897
Lehninger 3.859
Nozaki 3.999
DTASelect 4.291
Thurlkill 3.719
EMBOSS 3.846
Sillero 4.024
Patrickios 1.341
IPC_peptide 3.897
IPC2_peptide 3.999
IPC2.peptide.svr19 3.899
Protein with the highest isoelectric point:
>tr|A0A1Y0IGN0|A0A1Y0IGN0_9GAMM Paraquat-inducible protein B OS=Oleiphilus messinensis OX=141451 GN=OLMES_5299 PE=4 SV=1
MM1 pKa = 7.94 AEE3 pKa = 4.12 FLSIGAAAFLLGVAVSTLRR22 pKa = 11.84 CWEE25 pKa = 3.88 KK26 pKa = 10.5 EE27 pKa = 3.8 SRR29 pKa = 11.84 FFSDD33 pKa = 3.57 FRR35 pKa = 11.84 TPGGHH40 pKa = 6.3 RR41 pKa = 11.84 RR42 pKa = 11.84 YY43 pKa = 10.62 ALDD46 pKa = 4.28 KK47 pKa = 10.97 LLAFCGQSTANEE59 pKa = 3.89 QRR61 pKa = 11.84 RR62 pKa = 11.84 TICYY66 pKa = 10.24 ARR68 pKa = 11.84 VSSHH72 pKa = 6.2 DD73 pKa = 3.59 QKK75 pKa = 11.66 KK76 pKa = 10.55 DD77 pKa = 3.21 LQTQIARR84 pKa = 11.84 LYY86 pKa = 9.97 GSRR89 pKa = 11.84 RR90 pKa = 11.84 RR91 pKa = 11.84 KK92 pKa = 7.75 NQRR95 pKa = 11.84 AIAA98 pKa = 3.94
Molecular weight: 11.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.604
IPC_protein 10.409
Toseland 10.57
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.409
Wikipedia 10.891
Rodwell 10.877
Grimsley 10.745
Solomon 10.789
Lehninger 10.76
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.628
IPC_peptide 10.804
IPC2_peptide 9.619
IPC2.peptide.svr19 8.502
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5456
0
5456
1829888
19
7866
335.4
37.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.581 ± 0.035
1.043 ± 0.012
5.663 ± 0.031
6.396 ± 0.033
4.108 ± 0.023
6.811 ± 0.04
2.305 ± 0.018
6.169 ± 0.024
4.736 ± 0.032
10.519 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.018
4.087 ± 0.024
4.238 ± 0.023
4.609 ± 0.023
5.284 ± 0.026
6.734 ± 0.028
5.382 ± 0.03
6.795 ± 0.032
1.253 ± 0.013
2.974 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here