Geodermatophilus sp. TF02-6
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4244 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A367A3L8|A0A367A3L8_9ACTN TerC family protein OS=Geodermatophilus sp. TF02-6 OX=2250575 GN=DQ238_03350 PE=3 SV=1
MM1 pKa = 7.6 AATPPRR7 pKa = 11.84 RR8 pKa = 11.84 TLAALLAAVALLLVSGCSIVVPGRR32 pKa = 11.84 PSAAQPPTDD41 pKa = 3.73 DD42 pKa = 3.59 VAPGEE47 pKa = 4.14 LAVAGATGGPIDD59 pKa = 3.82 TLARR63 pKa = 11.84 NALTDD68 pKa = 4.66 LEE70 pKa = 4.96 TYY72 pKa = 8.72 WAGQFPDD79 pKa = 4.15 VFGQPFQPLQGGYY92 pKa = 10.53 FSVDD96 pKa = 3.44 PGNVAPGEE104 pKa = 4.13 FPQGIGCGADD114 pKa = 3.38 PLDD117 pKa = 3.7 VEE119 pKa = 5.82 GNAFYY124 pKa = 10.7 CQASDD129 pKa = 4.2 APNSDD134 pKa = 3.73 SISYY138 pKa = 10.51 DD139 pKa = 3.0 RR140 pKa = 11.84 AFLQEE145 pKa = 4.66 LADD148 pKa = 3.44 QYY150 pKa = 11.9 GRR152 pKa = 11.84 FIPALVMAHH161 pKa = 6.2 EE162 pKa = 5.31 FGHH165 pKa = 6.69 AVQARR170 pKa = 11.84 VGYY173 pKa = 8.38 PDD175 pKa = 3.67 YY176 pKa = 10.82 SISVEE181 pKa = 4.12 TQADD185 pKa = 4.0 CFAGAWTAWVADD197 pKa = 3.98 GQAEE201 pKa = 4.23 HH202 pKa = 6.15 SQIRR206 pKa = 11.84 APEE209 pKa = 4.1 LDD211 pKa = 3.48 EE212 pKa = 4.08 VLRR215 pKa = 11.84 GYY217 pKa = 11.13 LLLRR221 pKa = 11.84 DD222 pKa = 3.92 PVGTSINTEE231 pKa = 4.01 AAHH234 pKa = 6.51 GSYY237 pKa = 10.46 FDD239 pKa = 3.78 RR240 pKa = 11.84 VSAFQEE246 pKa = 4.33 GFEE249 pKa = 4.8 AGPTACRR256 pKa = 11.84 DD257 pKa = 3.43 DD258 pKa = 4.05 FTAQRR263 pKa = 11.84 PYY265 pKa = 10.15 TQGAFQDD272 pKa = 3.78 PSEE275 pKa = 4.33 ARR277 pKa = 11.84 TGGNSSFRR285 pKa = 11.84 EE286 pKa = 4.18 AQDD289 pKa = 2.64 IAATVLPEE297 pKa = 3.83 FWNRR301 pKa = 11.84 AFTEE305 pKa = 4.2 VFDD308 pKa = 4.31 ATFSPPTLEE317 pKa = 4.72 PFTGTAPSCAPDD329 pKa = 4.37 DD330 pKa = 4.52 LDD332 pKa = 5.81 LVFCADD338 pKa = 4.01 EE339 pKa = 4.23 NLVGYY344 pKa = 9.92 DD345 pKa = 3.99 EE346 pKa = 4.83 QDD348 pKa = 3.34 LAAPAYY354 pKa = 9.13 QQIGDD359 pKa = 4.07 YY360 pKa = 10.96 AVVTAASLPYY370 pKa = 9.28 AQSGRR375 pKa = 11.84 EE376 pKa = 3.75 QLGRR380 pKa = 11.84 STDD383 pKa = 3.42 DD384 pKa = 3.02 EE385 pKa = 4.23 AAIRR389 pKa = 11.84 SAVCLTGWFSAAFFDD404 pKa = 5.63 GALQSARR411 pKa = 11.84 ISPGDD416 pKa = 3.3 IDD418 pKa = 4.53 EE419 pKa = 4.65 SVQFLLEE426 pKa = 4.13 YY427 pKa = 9.56 GTDD430 pKa = 3.41 PSVFPDD436 pKa = 2.99 VDD438 pKa = 3.65 LTGFQLVDD446 pKa = 3.55 LFRR449 pKa = 11.84 NGFFDD454 pKa = 4.68 GAAACDD460 pKa = 3.64 VGVV463 pKa = 3.59
Molecular weight: 49.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.821
IPC_protein 3.846
Toseland 3.617
ProMoST 3.999
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.668
Grimsley 3.528
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.202
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.961
Patrickios 1.1
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.866
Protein with the highest isoelectric point:
>tr|A0A366ZDI2|A0A366ZDI2_9ACTN VWFA domain-containing protein OS=Geodermatophilus sp. TF02-6 OX=2250575 GN=DQ238_20375 PE=4 SV=1
MM1 pKa = 7.3 SGRR4 pKa = 11.84 RR5 pKa = 11.84 GSGRR9 pKa = 11.84 GMAGWAMSGRR19 pKa = 11.84 GLSGRR24 pKa = 11.84 GGSGRR29 pKa = 11.84 GMAGRR34 pKa = 11.84 GMAGWAMSGRR44 pKa = 11.84 GASAGRR50 pKa = 11.84 GHH52 pKa = 6.91 GRR54 pKa = 11.84 PTSRR58 pKa = 11.84 GMRR61 pKa = 11.84 RR62 pKa = 11.84 GRR64 pKa = 11.84 PTTAASGLGGVLSGLLRR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 3.65
Molecular weight: 8.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.433
IPC_protein 13.042
Toseland 13.203
ProMoST 13.7
Dawson 13.203
Bjellqvist 13.203
Wikipedia 13.685
Rodwell 12.705
Grimsley 13.247
Solomon 13.7
Lehninger 13.598
Nozaki 13.203
DTASelect 13.203
Thurlkill 13.203
EMBOSS 13.7
Sillero 13.203
Patrickios 12.427
IPC_peptide 13.7
IPC2_peptide 12.691
IPC2.peptide.svr19 9.338
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4244
0
4244
1348583
20
1980
317.8
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.467 ± 0.067
0.736 ± 0.01
6.251 ± 0.027
5.48 ± 0.039
2.5 ± 0.021
9.663 ± 0.036
2.081 ± 0.018
2.501 ± 0.025
1.197 ± 0.018
10.611 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.499 ± 0.015
1.334 ± 0.015
6.439 ± 0.033
2.677 ± 0.021
8.682 ± 0.038
4.608 ± 0.025
5.925 ± 0.029
10.098 ± 0.038
1.48 ± 0.017
1.769 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here