Flavihumibacter sp. SB-02

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Phnomibacter

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3417 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I6G8L2|A0A6I6G8L2_9BACT Tetratricopeptide repeat protein OS=Flavihumibacter sp. SB-02 OX=2676868 GN=GLV81_06575 PE=4 SV=1
MM1 pKa = 7.22KK2 pKa = 10.35QLLTIFLLLVHH13 pKa = 6.49VNSAMLLPQVEE24 pKa = 4.39AHH26 pKa = 6.14TATNSIGSTTDD37 pKa = 3.22VINSIYY43 pKa = 10.49EE44 pKa = 4.17WVDD47 pKa = 3.07EE48 pKa = 4.37TLLDD52 pKa = 3.67HH53 pKa = 7.18HH54 pKa = 7.55DD55 pKa = 4.09SSTQDD60 pKa = 2.96KK61 pKa = 10.8HH62 pKa = 9.13DD63 pKa = 4.66EE64 pKa = 4.25DD65 pKa = 6.21DD66 pKa = 4.02GLQIEE71 pKa = 4.79TGIADD76 pKa = 4.14DD77 pKa = 3.73FCSRR81 pKa = 11.84LFYY84 pKa = 10.55LAQIEE89 pKa = 4.63SPLAFTNSHH98 pKa = 6.32NATTHH103 pKa = 5.77AAGALEE109 pKa = 4.23MSYY112 pKa = 10.75EE113 pKa = 4.39LNTPPPEE120 pKa = 4.07VV121 pKa = 3.35

Molecular weight: 13.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I6GAR4|A0A6I6GAR4_9BACT NgoFVII family restriction endonuclease OS=Flavihumibacter sp. SB-02 OX=2676868 GN=GLV81_12055 PE=4 SV=1
MM1 pKa = 6.54FTHH4 pKa = 6.49VALFSRR10 pKa = 11.84PISCRR15 pKa = 11.84IFVWQKK21 pKa = 9.16QSCSLIVSLVTTSNQQAARR40 pKa = 11.84AAIGGWGEE48 pKa = 3.95IQHH51 pKa = 5.73TCSVEE56 pKa = 3.85LLFVQQLFIGWKK68 pKa = 9.47NEE70 pKa = 3.37QLLPYY75 pKa = 10.18RR76 pKa = 11.84PGWNKK81 pKa = 10.25AGKK84 pKa = 9.81DD85 pKa = 3.18AVTNTRR91 pKa = 11.84GGQSTRR97 pKa = 11.84LRR99 pKa = 11.84LTLTFLFLGLGVTFRR114 pKa = 11.84ISLGVV119 pKa = 3.2

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3417

0

3417

1144813

31

2422

335.0

37.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.784 ± 0.048

0.926 ± 0.014

4.938 ± 0.036

5.024 ± 0.045

4.624 ± 0.027

7.024 ± 0.042

2.157 ± 0.024

6.195 ± 0.032

5.832 ± 0.038

9.468 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.024

5.171 ± 0.043

4.164 ± 0.023

4.838 ± 0.039

4.138 ± 0.024

6.142 ± 0.04

5.858 ± 0.045

6.614 ± 0.031

1.536 ± 0.02

3.889 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski