Amino acid dipepetide frequency for Flavihumibacter sp. SB-02

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	9.802AlaAla: 9.802 ± 0.13	0.879AlaCys: 0.879 ± 0.026	4.943AlaAsp: 4.943 ± 0.067	4.423AlaGlu: 4.423 ± 0.063	3.883AlaPhe: 3.883 ± 0.06	7.07AlaGly: 7.07 ± 0.098	1.55AlaHis: 1.55 ± 0.036	5.44AlaIle: 5.44 ± 0.072	4.553AlaLys: 4.553 ± 0.074	7.697AlaLeu: 7.697 ± 0.095	2.427AlaMet: 2.427 ± 0.06	4.427AlaAsn: 4.427 ± 0.072	3.034AlaPro: 3.034 ± 0.066	4.135AlaGln: 4.135 ± 0.061	2.859AlaArg: 2.859 ± 0.05	5.288AlaSer: 5.288 ± 0.074	5.203AlaThr: 5.203 ± 0.096	5.989AlaVal: 5.989 ± 0.076	1.21AlaTrp: 1.21 ± 0.033	3.03AlaTyr: 3.03 ± 0.061	0.0AlaXaa: 0.0 ± 0.0
Cys	0.627CysAla: 0.627 ± 0.021	0.229CysCys: 0.229 ± 0.015	0.402CysAsp: 0.402 ± 0.02	0.391CysGlu: 0.391 ± 0.016	0.466CysPhe: 0.466 ± 0.023	0.756CysGly: 0.756 ± 0.029	0.251CysHis: 0.251 ± 0.017	0.665CysIle: 0.665 ± 0.023	0.472CysLys: 0.472 ± 0.021	0.798CysLeu: 0.798 ± 0.027	0.292CysMet: 0.292 ± 0.017	0.466CysAsn: 0.466 ± 0.022	0.392CysPro: 0.392 ± 0.018	0.31CysGln: 0.31 ± 0.018	0.395CysArg: 0.395 ± 0.02	0.672CysSer: 0.672 ± 0.028	0.601CysThr: 0.601 ± 0.023	0.58CysVal: 0.58 ± 0.025	0.148CysTrp: 0.148 ± 0.011	0.349CysTyr: 0.349 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	4.913AspAla: 4.913 ± 0.068	0.441AspCys: 0.441 ± 0.02	2.304AspAsp: 2.304 ± 0.058	2.809AspGlu: 2.809 ± 0.065	2.586AspPhe: 2.586 ± 0.052	3.891AspGly: 3.891 ± 0.101	0.825AspHis: 0.825 ± 0.028	3.35AspIle: 3.35 ± 0.06	2.963AspLys: 2.963 ± 0.059	3.962AspLeu: 3.962 ± 0.067	1.297AspMet: 1.297 ± 0.032	2.466AspAsn: 2.466 ± 0.057	1.87AspPro: 1.87 ± 0.043	1.301AspGln: 1.301 ± 0.034	1.981AspArg: 1.981 ± 0.044	3.216AspSer: 3.216 ± 0.068	2.697AspThr: 2.697 ± 0.059	3.522AspVal: 3.522 ± 0.06	0.829AspTrp: 0.829 ± 0.026	2.158AspTyr: 2.158 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	4.597GluAla: 4.597 ± 0.084	0.363GluCys: 0.363 ± 0.019	2.137GluAsp: 2.137 ± 0.055	3.158GluGlu: 3.158 ± 0.072	1.932GluPhe: 1.932 ± 0.042	3.145GluGly: 3.145 ± 0.051	1.086GluHis: 1.086 ± 0.034	3.101GluIle: 3.101 ± 0.064	4.11GluLys: 4.11 ± 0.064	4.927GluLeu: 4.927 ± 0.083	1.521GluMet: 1.521 ± 0.038	2.38GluAsn: 2.38 ± 0.048	1.684GluPro: 1.684 ± 0.04	2.63GluGln: 2.63 ± 0.059	2.189GluArg: 2.189 ± 0.049	2.386GluSer: 2.386 ± 0.042	2.713GluThr: 2.713 ± 0.053	3.505GluVal: 3.505 ± 0.066	0.863GluTrp: 0.863 ± 0.028	1.818GluTyr: 1.818 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	3.995PheAla: 3.995 ± 0.056	0.445PheCys: 0.445 ± 0.021	2.835PheAsp: 2.835 ± 0.047	2.49PheGlu: 2.49 ± 0.049	2.337PhePhe: 2.337 ± 0.049	3.428PheGly: 3.428 ± 0.061	0.854PheHis: 0.854 ± 0.029	2.848PheIle: 2.848 ± 0.053	2.276PheLys: 2.276 ± 0.052	3.732PheLeu: 3.732 ± 0.066	1.128PheMet: 1.128 ± 0.031	2.446PheAsn: 2.446 ± 0.058	1.715PhePro: 1.715 ± 0.036	1.616PheGln: 1.616 ± 0.037	1.916PheArg: 1.916 ± 0.043	3.295PheSer: 3.295 ± 0.057	3.206PheThr: 3.206 ± 0.067	3.167PheVal: 3.167 ± 0.058	0.66PheTrp: 0.66 ± 0.029	1.856PheTyr: 1.856 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	5.133GlyAla: 5.133 ± 0.086	0.761GlyCys: 0.761 ± 0.035	3.247GlyAsp: 3.247 ± 0.049	3.269GlyGlu: 3.269 ± 0.057	3.64GlyPhe: 3.64 ± 0.059	5.179GlyGly: 5.179 ± 0.122	1.408GlyHis: 1.408 ± 0.036	5.046GlyIle: 5.046 ± 0.067	5.004GlyLys: 5.004 ± 0.079	6.166GlyLeu: 6.166 ± 0.085	2.04GlyMet: 2.04 ± 0.049	3.956GlyAsn: 3.956 ± 0.084	1.772GlyPro: 1.772 ± 0.045	2.738GlyGln: 2.738 ± 0.054	2.843GlyArg: 2.843 ± 0.049	4.651GlySer: 4.651 ± 0.082	4.628GlyThr: 4.628 ± 0.1	4.518GlyVal: 4.518 ± 0.085	1.261GlyTrp: 1.261 ± 0.039	2.977GlyTyr: 2.977 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.686HisAla: 1.686 ± 0.041	0.273HisCys: 0.273 ± 0.015	0.913HisAsp: 0.913 ± 0.029	1.03HisGlu: 1.03 ± 0.03	1.28HisPhe: 1.28 ± 0.032	1.354HisGly: 1.354 ± 0.041	0.69HisHis: 0.69 ± 0.029	1.467HisIle: 1.467 ± 0.039	1.03HisLys: 1.03 ± 0.034	2.079HisLeu: 2.079 ± 0.046	0.535HisMet: 0.535 ± 0.023	0.973HisAsn: 0.973 ± 0.032	1.244HisPro: 1.244 ± 0.032	1.062HisGln: 1.062 ± 0.033	0.971HisArg: 0.971 ± 0.028	1.181HisSer: 1.181 ± 0.036	1.138HisThr: 1.138 ± 0.035	1.27HisVal: 1.27 ± 0.036	0.414HisTrp: 0.414 ± 0.018	0.978HisTyr: 0.978 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.937IleAla: 5.937 ± 0.085	0.682IleCys: 0.682 ± 0.026	3.82IleAsp: 3.82 ± 0.064	3.65IleGlu: 3.65 ± 0.064	2.474IlePhe: 2.474 ± 0.062	4.424IleGly: 4.424 ± 0.071	1.411IleHis: 1.411 ± 0.04	3.863IleIle: 3.863 ± 0.066	3.252IleLys: 3.252 ± 0.062	4.836IleLeu: 4.836 ± 0.073	1.16IleMet: 1.16 ± 0.038	3.352IleAsn: 3.352 ± 0.058	2.778IlePro: 2.778 ± 0.051	2.525IleGln: 2.525 ± 0.048	2.911IleArg: 2.911 ± 0.051	4.159IleSer: 4.159 ± 0.059	3.88IleThr: 3.88 ± 0.067	4.181IleVal: 4.181 ± 0.067	0.727IleTrp: 0.727 ± 0.025	1.933IleTyr: 1.933 ± 0.042	0.0IleXaa: 0.0 ± 0.0
Lys	5.358LysAla: 5.358 ± 0.086	0.345LysCys: 0.345 ± 0.019	3.137LysAsp: 3.137 ± 0.058	3.541LysGlu: 3.541 ± 0.063	1.956LysPhe: 1.956 ± 0.046	4.118LysGly: 4.118 ± 0.073	1.239LysHis: 1.239 ± 0.037	3.208LysIle: 3.208 ± 0.062	4.217LysLys: 4.217 ± 0.078	5.238LysLeu: 5.238 ± 0.069	1.761LysMet: 1.761 ± 0.044	2.796LysAsn: 2.796 ± 0.053	2.732LysPro: 2.732 ± 0.053	3.101LysGln: 3.101 ± 0.058	2.501LysArg: 2.501 ± 0.045	2.92LysSer: 2.92 ± 0.049	3.416LysThr: 3.416 ± 0.054	3.689LysVal: 3.689 ± 0.068	0.886LysTrp: 0.886 ± 0.029	2.161LysTyr: 2.161 ± 0.046	0.0LysXaa: 0.0 ± 0.0
Leu	7.571LeuAla: 7.571 ± 0.105	0.923LeuCys: 0.923 ± 0.027	4.112LeuAsp: 4.112 ± 0.072	4.321LeuGlu: 4.321 ± 0.073	4.235LeuPhe: 4.235 ± 0.073	5.582LeuGly: 5.582 ± 0.077	2.399LeuHis: 2.399 ± 0.049	5.046LeuIle: 5.046 ± 0.071	5.284LeuLys: 5.284 ± 0.083	11.034LeuLeu: 11.034 ± 0.143	2.646LeuMet: 2.646 ± 0.057	4.581LeuAsn: 4.581 ± 0.075	4.942LeuPro: 4.942 ± 0.072	6.259LeuGln: 6.259 ± 0.088	4.271LeuArg: 4.271 ± 0.065	6.226LeuSer: 6.226 ± 0.08	4.95LeuThr: 4.95 ± 0.068	5.647LeuVal: 5.647 ± 0.073	1.129LeuTrp: 1.129 ± 0.033	3.519LeuTyr: 3.519 ± 0.054	0.0LeuXaa: 0.0 ± 0.0
Met	2.455MetAla: 2.455 ± 0.047	0.188MetCys: 0.188 ± 0.013	1.142MetAsp: 1.142 ± 0.031	1.365MetGlu: 1.365 ± 0.034	0.923MetPhe: 0.923 ± 0.029	1.817MetGly: 1.817 ± 0.042	0.681MetHis: 0.681 ± 0.028	1.326MetIle: 1.326 ± 0.035	1.929MetLys: 1.929 ± 0.039	2.854MetLeu: 2.854 ± 0.058	0.866MetMet: 0.866 ± 0.029	1.274MetAsn: 1.274 ± 0.032	1.432MetPro: 1.432 ± 0.039	1.783MetGln: 1.783 ± 0.045	1.224MetArg: 1.224 ± 0.037	1.503MetSer: 1.503 ± 0.039	1.248MetThr: 1.248 ± 0.031	1.625MetVal: 1.625 ± 0.036	0.284MetTrp: 0.284 ± 0.016	0.855MetTyr: 0.855 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	4.43AsnAla: 4.43 ± 0.067	0.453AsnCys: 0.453 ± 0.02	2.676AsnAsp: 2.676 ± 0.065	2.655AsnGlu: 2.655 ± 0.05	2.179AsnPhe: 2.179 ± 0.051	4.319AsnGly: 4.319 ± 0.095	0.97AsnHis: 0.97 ± 0.031	3.381AsnIle: 3.381 ± 0.062	2.804AsnLys: 2.804 ± 0.059	4.096AsnLeu: 4.096 ± 0.07	1.324AsnMet: 1.324 ± 0.035	3.238AsnAsn: 3.238 ± 0.076	2.612AsnPro: 2.612 ± 0.062	1.963AsnGln: 1.963 ± 0.043	2.244AsnArg: 2.244 ± 0.05	3.172AsnSer: 3.172 ± 0.07	3.174AsnThr: 3.174 ± 0.069	3.092AsnVal: 3.092 ± 0.061	0.848AsnTrp: 0.848 ± 0.026	2.074AsnTyr: 2.074 ± 0.046	0.0AsnXaa: 0.0 ± 0.0
Pro	4.577ProAla: 4.577 ± 0.076	0.28ProCys: 0.28 ± 0.015	2.472ProAsp: 2.472 ± 0.049	2.448ProGlu: 2.448 ± 0.048	2.096ProPhe: 2.096 ± 0.044	3.066ProGly: 3.066 ± 0.053	0.824ProHis: 0.824 ± 0.031	2.22ProIle: 2.22 ± 0.043	2.093ProLys: 2.093 ± 0.044	3.633ProLeu: 3.633 ± 0.06	1.059ProMet: 1.059 ± 0.032	2.255ProAsn: 2.255 ± 0.052	1.253ProPro: 1.253 ± 0.045	1.742ProGln: 1.742 ± 0.041	1.209ProArg: 1.209 ± 0.031	2.249ProSer: 2.249 ± 0.043	2.41ProThr: 2.41 ± 0.057	3.589ProVal: 3.589 ± 0.062	0.549ProTrp: 0.549 ± 0.02	1.618ProTyr: 1.618 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	3.764GlnAla: 3.764 ± 0.067	0.356GlnCys: 0.356 ± 0.018	1.662GlnAsp: 1.662 ± 0.036	2.164GlnGlu: 2.164 ± 0.051	2.154GlnPhe: 2.154 ± 0.046	2.349GlnGly: 2.349 ± 0.051	1.478GlnHis: 1.478 ± 0.042	2.356GlnIle: 2.356 ± 0.04	2.358GlnLys: 2.358 ± 0.044	5.959GlnLeu: 5.959 ± 0.079	1.307GlnMet: 1.307 ± 0.034	1.825GlnAsn: 1.825 ± 0.048	2.491GlnPro: 2.491 ± 0.042	4.561GlnGln: 4.561 ± 0.093	2.233GlnArg: 2.233 ± 0.045	2.648GlnSer: 2.648 ± 0.05	2.666GlnThr: 2.666 ± 0.053	2.951GlnVal: 2.951 ± 0.051	0.974GlnTrp: 0.974 ± 0.037	2.125GlnTyr: 2.125 ± 0.045	0.0GlnXaa: 0.0 ± 0.0
Arg	2.255ArgAla: 2.255 ± 0.047	0.349ArgCys: 0.349 ± 0.018	1.704ArgAsp: 1.704 ± 0.041	1.843ArgGlu: 1.843 ± 0.042	2.408ArgPhe: 2.408 ± 0.047	2.282ArgGly: 2.282 ± 0.049	0.969ArgHis: 0.969 ± 0.034	3.393ArgIle: 3.393 ± 0.057	2.773ArgLys: 2.773 ± 0.05	4.219ArgLeu: 4.219 ± 0.064	1.351ArgMet: 1.351 ± 0.032	2.463ArgAsn: 2.463 ± 0.05	1.527ArgPro: 1.527 ± 0.038	2.25ArgGln: 2.25 ± 0.046	1.951ArgArg: 1.951 ± 0.038	2.52ArgSer: 2.52 ± 0.047	2.077ArgThr: 2.077 ± 0.043	2.319ArgVal: 2.319 ± 0.051	0.792ArgTrp: 0.792 ± 0.026	1.939ArgTyr: 1.939 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	4.808SerAla: 4.808 ± 0.076	0.582SerCys: 0.582 ± 0.024	2.655SerAsp: 2.655 ± 0.056	2.407SerGlu: 2.407 ± 0.045	3.452SerPhe: 3.452 ± 0.056	4.746SerGly: 4.746 ± 0.077	1.125SerHis: 1.125 ± 0.031	4.659SerIle: 4.659 ± 0.069	3.531SerLys: 3.531 ± 0.06	5.97SerLeu: 5.97 ± 0.067	1.732SerMet: 1.732 ± 0.041	3.617SerAsn: 3.617 ± 0.076	2.275SerPro: 2.275 ± 0.046	1.971SerGln: 1.971 ± 0.046	2.376SerArg: 2.376 ± 0.049	4.217SerSer: 4.217 ± 0.079	3.807SerThr: 3.807 ± 0.071	4.134SerVal: 4.134 ± 0.067	1.013SerTrp: 1.013 ± 0.03	2.342SerTyr: 2.342 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	5.798ThrAla: 5.798 ± 0.089	0.455ThrCys: 0.455 ± 0.021	3.195ThrAsp: 3.195 ± 0.065	2.64ThrGlu: 2.64 ± 0.059	2.346ThrPhe: 2.346 ± 0.051	5.112ThrGly: 5.112 ± 0.081	1.161ThrHis: 1.161 ± 0.035	3.845ThrIle: 3.845 ± 0.068	2.661ThrLys: 2.661 ± 0.055	5.282ThrLeu: 5.282 ± 0.074	1.177ThrMet: 1.177 ± 0.033	2.98ThrAsn: 2.98 ± 0.069	2.89ThrPro: 2.89 ± 0.058	2.338ThrGln: 2.338 ± 0.044	1.99ThrArg: 1.99 ± 0.042	3.492ThrSer: 3.492 ± 0.066	3.833ThrThr: 3.833 ± 0.087	4.382ThrVal: 4.382 ± 0.085	0.82ThrTrp: 0.82 ± 0.032	2.186ThrTyr: 2.186 ± 0.054	0.0ThrXaa: 0.0 ± 0.0
Val	6.115ValAla: 6.115 ± 0.087	0.698ValCys: 0.698 ± 0.029	3.198ValAsp: 3.198 ± 0.05	3.068ValGlu: 3.068 ± 0.059	3.106ValPhe: 3.106 ± 0.052	4.071ValGly: 4.071 ± 0.067	1.348ValHis: 1.348 ± 0.038	3.871ValIle: 3.871 ± 0.061	3.801ValLys: 3.801 ± 0.063	6.803ValLeu: 6.803 ± 0.086	1.719ValMet: 1.719 ± 0.041	3.167ValAsn: 3.167 ± 0.058	2.848ValPro: 2.848 ± 0.051	3.191ValGln: 3.191 ± 0.057	2.575ValArg: 2.575 ± 0.055	4.228ValSer: 4.228 ± 0.068	3.857ValThr: 3.857 ± 0.071	4.908ValVal: 4.908 ± 0.081	0.956ValTrp: 0.956 ± 0.033	2.612ValTyr: 2.612 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.963TrpAla: 0.963 ± 0.029	0.144TrpCys: 0.144 ± 0.013	0.707TrpAsp: 0.707 ± 0.024	0.698TrpGlu: 0.698 ± 0.025	0.641TrpPhe: 0.641 ± 0.021	0.919TrpGly: 0.919 ± 0.031	0.41TrpHis: 0.41 ± 0.019	0.783TrpIle: 0.783 ± 0.025	0.963TrpLys: 0.963 ± 0.032	1.813TrpLeu: 1.813 ± 0.046	0.59TrpMet: 0.59 ± 0.023	0.802TrpAsn: 0.802 ± 0.027	0.517TrpPro: 0.517 ± 0.025	1.137TrpGln: 1.137 ± 0.041	0.75TrpArg: 0.75 ± 0.026	0.865TrpSer: 0.865 ± 0.027	0.779TrpThr: 0.779 ± 0.032	0.925TrpVal: 0.925 ± 0.033	0.33TrpTrp: 0.33 ± 0.017	0.619TrpTyr: 0.619 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.056TyrAla: 3.056 ± 0.055	0.416TyrCys: 0.416 ± 0.021	2.123TyrAsp: 2.123 ± 0.049	1.874TyrGlu: 1.874 ± 0.042	2.15TyrPhe: 2.15 ± 0.048	2.61TyrGly: 2.61 ± 0.052	0.892TyrHis: 0.892 ± 0.024	2.087TyrIle: 2.087 ± 0.043	2.247TyrLys: 2.247 ± 0.048	3.581TyrLeu: 3.581 ± 0.063	0.84TyrMet: 0.84 ± 0.024	2.242TyrAsn: 2.242 ± 0.051	1.633TyrPro: 1.633 ± 0.039	1.767TyrGln: 1.767 ± 0.045	1.994TyrArg: 1.994 ± 0.045	2.531TyrSer: 2.531 ± 0.055	2.297TyrThr: 2.297 ± 0.048	2.147TyrVal: 2.147 ± 0.044	0.662TyrTrp: 0.662 ± 0.023	1.742TyrTyr: 1.742 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3417 proteins (1144813 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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