Porcine adenovirus A serotype 3 (PAdV-3) (Porcine adenovirus 3)
Average proteome isoelectric point is 5.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9W8X7|Q9W8X7_ADEP3 PTP (COOH region) (Fragment) OS=Porcine adenovirus A serotype 3 OX=35265 PE=3 SV=1
MM1 pKa = 7.86 ANRR4 pKa = 11.84 LHH6 pKa = 7.12 LDD8 pKa = 2.82 WDD10 pKa = 4.16 GNPEE14 pKa = 4.1 VVPVLEE20 pKa = 4.5 WDD22 pKa = 3.75 PVDD25 pKa = 5.69 LRR27 pKa = 11.84 DD28 pKa = 4.79 PSPGDD33 pKa = 3.23 EE34 pKa = 4.89 GFCEE38 pKa = 4.54 PCWEE42 pKa = 4.29 SLVDD46 pKa = 3.75 GLPDD50 pKa = 3.19 EE51 pKa = 4.78 WLDD54 pKa = 3.68 SVDD57 pKa = 3.59 EE58 pKa = 4.47 VEE60 pKa = 5.07 VIVTEE65 pKa = 4.45 GGEE68 pKa = 4.33 SEE70 pKa = 4.94 DD71 pKa = 4.06 SGGSAAGDD79 pKa = 3.42 SGGSQGVFEE88 pKa = 4.85 MDD90 pKa = 3.39 PPEE93 pKa = 4.52 EE94 pKa = 4.58 GDD96 pKa = 3.89 SNEE99 pKa = 3.92 EE100 pKa = 4.38 DD101 pKa = 3.2 ISAVAAEE108 pKa = 4.51 VLSEE112 pKa = 3.91 LADD115 pKa = 3.65 VVFEE119 pKa = 5.31 DD120 pKa = 4.72 PLAPPSPFVLDD131 pKa = 3.88 CPEE134 pKa = 4.01 VPGVNCRR141 pKa = 11.84 SCDD144 pKa = 3.35 YY145 pKa = 11.05 HH146 pKa = 8.04 RR147 pKa = 11.84 FHH149 pKa = 7.96 SKK151 pKa = 10.75 DD152 pKa = 3.49 PNLKK156 pKa = 10.46 CSLCYY161 pKa = 10.16 MRR163 pKa = 11.84 MHH165 pKa = 7.02 AFAVYY170 pKa = 9.94 GEE172 pKa = 4.6 CFWTFVGLCGKK183 pKa = 9.72 KK184 pKa = 10.47 GKK186 pKa = 9.03 SACKK190 pKa = 9.41 KK191 pKa = 9.06 SHH193 pKa = 5.01 VLFPIFCLFRR203 pKa = 11.84 SCFSSTSQVGFPGTWRR219 pKa = 11.84 PARR222 pKa = 11.84 TQEE225 pKa = 4.14 EE226 pKa = 4.7 VLLL229 pKa = 4.34
Molecular weight: 25.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.975
IPC2_protein 4.126
IPC_protein 4.088
Toseland 3.91
ProMoST 4.215
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.948
Rodwell 3.923
Grimsley 3.821
Solomon 4.05
Lehninger 3.999
Nozaki 4.164
DTASelect 4.342
Thurlkill 3.935
EMBOSS 3.961
Sillero 4.202
Patrickios 2.587
IPC_peptide 4.05
IPC2_peptide 4.19
IPC2.peptide.svr19 4.095
Protein with the highest isoelectric point:
>tr|Q9W8S1|Q9W8S1_ADEP3 Isoform of Q84177 Hexon protein OS=Porcine adenovirus A serotype 3 OX=35265 GN=L3 PE=2 SV=1
MM1 pKa = 7.9 AILVSPSNNSGWGLGLRR18 pKa = 11.84 SMYY21 pKa = 10.65 GGARR25 pKa = 11.84 RR26 pKa = 11.84 LSPDD30 pKa = 2.67 HH31 pKa = 6.08 PVIVRR36 pKa = 11.84 RR37 pKa = 11.84 HH38 pKa = 4.92 YY39 pKa = 8.16 RR40 pKa = 11.84 TNWGSLKK47 pKa = 10.49 GRR49 pKa = 11.84 VAPSTIATTDD59 pKa = 3.49 DD60 pKa = 3.48 PVADD64 pKa = 4.0 VVNAIAGATRR74 pKa = 11.84 RR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 HH79 pKa = 4.42 RR80 pKa = 11.84 RR81 pKa = 11.84 RR82 pKa = 11.84 RR83 pKa = 11.84 RR84 pKa = 11.84 AARR87 pKa = 11.84 VSSVAVTGDD96 pKa = 3.39 PVADD100 pKa = 3.41 VVNAVEE106 pKa = 4.0 AVARR110 pKa = 11.84 RR111 pKa = 11.84 RR112 pKa = 11.84 RR113 pKa = 11.84 ARR115 pKa = 11.84 RR116 pKa = 11.84 RR117 pKa = 11.84 SSRR120 pKa = 11.84 MQTTGDD126 pKa = 3.47 PVADD130 pKa = 3.36 VVAAVEE136 pKa = 4.03 AVARR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 RR143 pKa = 11.84 STRR146 pKa = 11.84 RR147 pKa = 11.84 RR148 pKa = 11.84 RR149 pKa = 11.84 RR150 pKa = 11.84 RR151 pKa = 11.84 SAPAILGVRR160 pKa = 11.84 RR161 pKa = 11.84 SRR163 pKa = 11.84 RR164 pKa = 11.84 LRR166 pKa = 11.84 KK167 pKa = 8.81 RR168 pKa = 11.84 TSSS171 pKa = 3.11
Molecular weight: 19.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.272
IPC2_protein 10.657
IPC_protein 12.193
Toseland 12.354
ProMoST 12.852
Dawson 12.354
Bjellqvist 12.354
Wikipedia 12.822
Rodwell 11.886
Grimsley 12.384
Solomon 12.852
Lehninger 12.749
Nozaki 12.354
DTASelect 12.354
Thurlkill 12.354
EMBOSS 12.852
Sillero 12.354
Patrickios 11.594
IPC_peptide 12.852
IPC2_peptide 11.842
IPC2.peptide.svr19 9.238
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
2
16
6661
171
938
416.3
46.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.677 ± 0.664
1.666 ± 0.356
5.33 ± 0.331
6.951 ± 0.697
3.948 ± 0.343
6.756 ± 0.646
2.147 ± 0.363
2.927 ± 0.313
2.282 ± 0.298
9.758 ± 0.518
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.327 ± 0.138
4.053 ± 0.607
6.876 ± 0.398
4.564 ± 0.349
7.792 ± 0.817
7.506 ± 0.58
5.69 ± 0.566
6.35 ± 0.415
1.216 ± 0.115
3.183 ± 0.46
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here