Sharpea azabuensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Coprobacillaceae; Sharpea

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2425 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H6YC29|A0A1H6YC29_9FIRM Oligopeptide transport system substrate-binding protein OS=Sharpea azabuensis OX=322505 GN=SAMN04487834_11181 PE=3 SV=1
FF1 pKa = 7.31LNSLKK6 pKa = 10.59RR7 pKa = 11.84LEE9 pKa = 4.24EE10 pKa = 3.77EE11 pKa = 5.97AYY13 pKa = 10.21DD14 pKa = 3.51AWGDD18 pKa = 4.17EE19 pKa = 4.34VWSPLDD25 pKa = 3.58GEE27 pKa = 4.59SYY29 pKa = 11.06YY30 pKa = 10.77VDD32 pKa = 3.28EE33 pKa = 4.75WRR35 pKa = 11.84HH36 pKa = 5.29EE37 pKa = 3.88IYY39 pKa = 10.23MVDD42 pKa = 3.14INGNEE47 pKa = 4.09WNLMMLGQYY56 pKa = 9.15II57 pKa = 3.73

Molecular weight: 6.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6U783|A0A1H6U783_9FIRM Cysteine--tRNA ligase OS=Sharpea azabuensis OX=322505 GN=cysS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.34VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.98RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.14ALTVV44 pKa = 3.39

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2425

0

2425

706142

39

1500

291.2

32.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.915 ± 0.051

1.199 ± 0.02

6.137 ± 0.05

6.769 ± 0.064

4.259 ± 0.037

6.358 ± 0.049

2.562 ± 0.029

8.05 ± 0.049

7.613 ± 0.051

9.108 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.103 ± 0.024

4.969 ± 0.04

3.054 ± 0.026

3.137 ± 0.028

3.844 ± 0.032

5.614 ± 0.034

5.29 ± 0.044

6.682 ± 0.046

0.72 ± 0.016

4.617 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski