Arthrobacter phage Bridgette

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bridgettevirus; Arthrobacter virus Bridgette

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 71 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2KEB7|A0A3G2KEB7_9CAUD Uncharacterized protein OS=Arthrobacter phage Bridgette OX=2419949 GN=68 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 5.23EE3 pKa = 4.82LWNDD7 pKa = 3.49SCPCGHH13 pKa = 6.8TWDD16 pKa = 3.55EE17 pKa = 4.49HH18 pKa = 6.8NFEE21 pKa = 4.57VGCIAGWEE29 pKa = 4.07YY30 pKa = 11.18DD31 pKa = 3.78GPDD34 pKa = 3.56PGIATKK40 pKa = 10.51DD41 pKa = 3.16GCACQLAHH49 pKa = 6.02TEE51 pKa = 4.13RR52 pKa = 11.84SRR54 pKa = 3.72

Molecular weight: 6.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2KEK2|A0A3G2KEK2_9CAUD HNH endonuclease OS=Arthrobacter phage Bridgette OX=2419949 GN=71 PE=4 SV=1
MM1 pKa = 7.27PHH3 pKa = 7.32APRR6 pKa = 11.84KK7 pKa = 7.99PCPTAGCPEE16 pKa = 4.88LLPPGQRR23 pKa = 11.84RR24 pKa = 11.84CPTHH28 pKa = 6.16ATQHH32 pKa = 5.03EE33 pKa = 4.42QTRR36 pKa = 11.84GSRR39 pKa = 11.84TQRR42 pKa = 11.84GYY44 pKa = 11.3DD45 pKa = 3.29ATHH48 pKa = 7.08DD49 pKa = 3.88ALRR52 pKa = 11.84KK53 pKa = 8.63QWAPQVATGAVRR65 pKa = 11.84CARR68 pKa = 11.84CGKK71 pKa = 9.28PILAGTPWDD80 pKa = 4.6LGHH83 pKa = 6.87TDD85 pKa = 6.05DD86 pKa = 4.01RR87 pKa = 11.84TTWTGPEE94 pKa = 3.42HH95 pKa = 6.52AACNRR100 pKa = 11.84RR101 pKa = 11.84AGGKK105 pKa = 7.21ATKK108 pKa = 10.08HH109 pKa = 5.55RR110 pKa = 11.84GHH112 pKa = 6.76RR113 pKa = 11.84RR114 pKa = 3.18

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

71

0

71

13767

28

1305

193.9

21.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.058 ± 0.606

0.966 ± 0.145

5.862 ± 0.185

5.702 ± 0.324

2.528 ± 0.143

8.716 ± 0.409

1.918 ± 0.239

4.249 ± 0.189

4.504 ± 0.301

7.838 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.099 ± 0.139

3.007 ± 0.189

5.942 ± 0.47

4.126 ± 0.349

6.494 ± 0.427

5.564 ± 0.312

7.293 ± 0.375

6.748 ± 0.278

1.838 ± 0.223

2.55 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski