Vagococcus carniphilus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2902 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430B8Y8|A0A430B8Y8_9ENTE Uncharacterized protein OS=Vagococcus carniphilus OX=218144 GN=CBF28_00900 PE=4 SV=1
MM1 pKa = 6.95TVAKK5 pKa = 9.77IVYY8 pKa = 10.16ASLTGNTEE16 pKa = 3.93EE17 pKa = 4.62IADD20 pKa = 3.75IVAEE24 pKa = 4.08ALEE27 pKa = 4.21SHH29 pKa = 6.37DD30 pKa = 4.09VEE32 pKa = 5.15VEE34 pKa = 4.2VNEE37 pKa = 4.31CTQVDD42 pKa = 3.47AADD45 pKa = 4.56FLDD48 pKa = 4.43ADD50 pKa = 3.71ICIVASYY57 pKa = 9.71TYY59 pKa = 11.12DD60 pKa = 4.21DD61 pKa = 4.96FLLPDD66 pKa = 4.53EE67 pKa = 5.72IIDD70 pKa = 3.79FHH72 pKa = 7.76EE73 pKa = 4.6EE74 pKa = 4.23LLDD77 pKa = 4.35LDD79 pKa = 4.25LTGKK83 pKa = 10.51VYY85 pKa = 10.68GVVGSGDD92 pKa = 2.93TFYY95 pKa = 10.58PYY97 pKa = 10.28FCQVVDD103 pKa = 3.83QFDD106 pKa = 3.42KK107 pKa = 11.49AFASVGATKK116 pKa = 10.34GAQSVKK122 pKa = 10.55VEE124 pKa = 4.31LAPEE128 pKa = 4.12EE129 pKa = 4.0QDD131 pKa = 3.54IVALQSFAKK140 pKa = 10.31ILVEE144 pKa = 4.36KK145 pKa = 10.84SKK147 pKa = 11.41LNN149 pKa = 3.61

Molecular weight: 16.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430ASQ9|A0A430ASQ9_9ENTE DNA replication and repair protein RecF OS=Vagococcus carniphilus OX=218144 GN=recF PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.94VLAAA44 pKa = 4.36

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2902

0

2902

910394

24

3430

313.7

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.999 ± 0.049

0.612 ± 0.011

5.407 ± 0.039

7.873 ± 0.056

4.672 ± 0.04

6.284 ± 0.051

1.689 ± 0.022

8.135 ± 0.046

8.074 ± 0.056

9.758 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.722 ± 0.03

5.145 ± 0.044

3.166 ± 0.025

3.593 ± 0.029

3.291 ± 0.029

6.485 ± 0.035

5.766 ± 0.032

6.837 ± 0.043

0.82 ± 0.016

3.671 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski