Streptococcus phage 2167
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8ZE31|E8ZE31_9CAUD Phage protein OS=Streptococcus phage 2167 OX=870475 PE=4 SV=1
MM1 pKa = 8.35 LMFDD5 pKa = 3.85 YY6 pKa = 10.77 DD7 pKa = 4.39 RR8 pKa = 11.84 DD9 pKa = 3.49 IMQPPEE15 pKa = 3.91 PRR17 pKa = 11.84 EE18 pKa = 3.89 EE19 pKa = 4.47 HH20 pKa = 7.45 DD21 pKa = 3.52 PADD24 pKa = 4.15 WIFSAGQWIYY34 pKa = 11.59 VGDD37 pKa = 4.05 CC38 pKa = 3.39
Molecular weight: 4.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.872
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 1.901
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|E8ZE26|E8ZE26_9CAUD Phage protein OS=Streptococcus phage 2167 OX=870475 PE=4 SV=1
MM1 pKa = 6.96 QRR3 pKa = 11.84 RR4 pKa = 11.84 EE5 pKa = 3.9 IMNDD9 pKa = 3.93 FIKK12 pKa = 10.46 EE13 pKa = 3.43 IGMAILWMFLGYY25 pKa = 10.88 LLGEE29 pKa = 4.21 RR30 pKa = 11.84 STRR33 pKa = 11.84 GGQIRR38 pKa = 3.63
Molecular weight: 4.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.201
IPC2_protein 9.253
IPC_protein 10.014
Toseland 10.028
ProMoST 10.716
Dawson 10.233
Bjellqvist 9.955
Wikipedia 10.467
Rodwell 10.321
Grimsley 10.321
Solomon 10.394
Lehninger 10.365
Nozaki 9.955
DTASelect 9.97
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.145
Patrickios 10.423
IPC_peptide 10.379
IPC2_peptide 8.799
IPC2.peptide.svr19 8.601
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
10685
38
1469
190.8
21.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.841 ± 0.632
0.674 ± 0.122
6.27 ± 0.307
8.563 ± 0.604
4.165 ± 0.302
6.523 ± 0.629
1.357 ± 0.165
6.504 ± 0.278
8.133 ± 0.348
8.198 ± 0.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.546 ± 0.217
5.185 ± 0.296
2.695 ± 0.255
4.296 ± 0.269
4.829 ± 0.372
5.962 ± 0.33
5.718 ± 0.294
6.514 ± 0.297
1.32 ± 0.167
3.706 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here