Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8955 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D2AZT6|D2AZT6_STRRD Prolyl oligopeptidase OS=Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100) OX=479432 GN=Sros_4277 PE=4 SV=1
MM1 pKa = 8.08 DD2 pKa = 4.51 LTGNDD7 pKa = 3.7 YY8 pKa = 11.25 SAGAAMTDD16 pKa = 3.47 YY17 pKa = 7.91 TTYY20 pKa = 10.39 TLWIVEE26 pKa = 4.02 GDD28 pKa = 3.57 AGQDD32 pKa = 3.37 SAGKK36 pKa = 10.09 DD37 pKa = 3.48 LFSPDD42 pKa = 3.6 SPIDD46 pKa = 3.79 YY47 pKa = 10.69 SDD49 pKa = 4.87 LNPAALLWLTEE60 pKa = 4.29 HH61 pKa = 7.0 GYY63 pKa = 10.62 EE64 pKa = 4.25 DD65 pKa = 3.39 PGRR68 pKa = 11.84 RR69 pKa = 11.84 VVVTPAGEE77 pKa = 4.23 VPKK80 pKa = 10.68 GAVIASYY87 pKa = 11.08 DD88 pKa = 3.44 VALL91 pKa = 4.47
Molecular weight: 9.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.745
IPC_protein 3.706
Toseland 3.49
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.859
DTASelect 4.113
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.834
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|D2BA27|D2BA27_STRRD Uncharacterized protein OS=Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100) OX=479432 GN=Sros_3092 PE=4 SV=1
MM1 pKa = 7.53 RR2 pKa = 11.84 PVRR5 pKa = 11.84 PVPWALRR12 pKa = 11.84 VLRR15 pKa = 11.84 VRR17 pKa = 11.84 PVLRR21 pKa = 11.84 VLRR24 pKa = 11.84 AQGVLWVRR32 pKa = 11.84 PVRR35 pKa = 11.84 PVPRR39 pKa = 11.84 VLRR42 pKa = 11.84 AQGVLRR48 pKa = 11.84 AQGVLRR54 pKa = 11.84 VRR56 pKa = 11.84 PVLRR60 pKa = 11.84 VWWALLVIRR69 pKa = 11.84 VLPVLPVRR77 pKa = 11.84 GVLRR81 pKa = 11.84 VTQVRR86 pKa = 11.84 RR87 pKa = 11.84 VLRR90 pKa = 11.84 ARR92 pKa = 11.84 LVRR95 pKa = 11.84 PVLQVLRR102 pKa = 11.84 VRR104 pKa = 11.84 EE105 pKa = 4.06 VLLVTT110 pKa = 4.82
Molecular weight: 12.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.896
Toseland 13.056
ProMoST 13.554
Dawson 13.056
Bjellqvist 13.056
Wikipedia 13.539
Rodwell 12.559
Grimsley 13.1
Solomon 13.554
Lehninger 13.466
Nozaki 13.056
DTASelect 13.056
Thurlkill 13.056
EMBOSS 13.554
Sillero 13.056
Patrickios 12.281
IPC_peptide 13.568
IPC2_peptide 12.559
IPC2.peptide.svr19 9.248
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8955
0
8955
2950138
30
7541
329.4
35.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.244 ± 0.033
0.77 ± 0.007
5.718 ± 0.022
5.637 ± 0.025
2.837 ± 0.015
9.612 ± 0.025
2.181 ± 0.012
3.619 ± 0.019
1.967 ± 0.022
10.591 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.908 ± 0.012
1.742 ± 0.015
6.115 ± 0.027
2.586 ± 0.016
8.218 ± 0.032
5.104 ± 0.016
5.832 ± 0.026
8.665 ± 0.026
1.566 ± 0.01
2.088 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here