Helicobacter phage FrB41M

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Schmidvirus; unclassified Schmidvirus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5RFD5|A0A1S5RFD5_9CAUD Putative tail fiber protein OS=Helicobacter phage FrB41M OX=1852663 PE=4 SV=1
MM1 pKa = 8.04AIRR4 pKa = 11.84EE5 pKa = 4.19KK6 pKa = 10.61EE7 pKa = 3.99IEE9 pKa = 4.22LEE11 pKa = 4.04TLKK14 pKa = 11.01RR15 pKa = 11.84EE16 pKa = 3.99IAQAEE21 pKa = 4.0ASLEE25 pKa = 3.98NDD27 pKa = 3.71FAKK30 pKa = 10.74HH31 pKa = 4.62MAEE34 pKa = 4.04KK35 pKa = 9.9TDD37 pKa = 3.73EE38 pKa = 4.15KK39 pKa = 11.43LEE41 pKa = 4.38DD42 pKa = 3.42LFFNNKK48 pKa = 7.78VDD50 pKa = 4.46FYY52 pKa = 11.51RR53 pKa = 11.84FILVEE58 pKa = 4.23QNDD61 pKa = 4.01FLNDD65 pKa = 4.17HH66 pKa = 6.83ISKK69 pKa = 10.53KK70 pKa = 9.59INKK73 pKa = 8.71AVALNEE79 pKa = 4.22EE80 pKa = 4.41IEE82 pKa = 4.15NSKK85 pKa = 10.45KK86 pKa = 10.03SQEE89 pKa = 3.9IEE91 pKa = 3.58EE92 pKa = 4.4AKK94 pKa = 10.82AKK96 pKa = 10.29FLEE99 pKa = 4.16KK100 pKa = 10.61HH101 pKa = 5.74KK102 pKa = 11.33GEE104 pKa = 4.51NIDD107 pKa = 3.92FNEE110 pKa = 4.24LVDD113 pKa = 4.91FYY115 pKa = 11.73NEE117 pKa = 4.12EE118 pKa = 4.07LPQKK122 pKa = 10.15YY123 pKa = 9.34KK124 pKa = 11.04RR125 pKa = 11.84EE126 pKa = 3.86IDD128 pKa = 3.59KK129 pKa = 11.24LDD131 pKa = 3.45GVQFFQSIYY140 pKa = 10.87DD141 pKa = 3.82LFKK144 pKa = 10.29ATQGEE149 pKa = 4.18NMQEE153 pKa = 3.62GKK155 pKa = 10.59AYY157 pKa = 10.36ARR159 pKa = 11.84EE160 pKa = 4.08EE161 pKa = 3.98EE162 pKa = 4.23RR163 pKa = 11.84NLPKK167 pKa = 10.14EE168 pKa = 4.07VKK170 pKa = 10.41GNGVSSSANARR181 pKa = 11.84NQSVMTRR188 pKa = 11.84FF189 pKa = 3.36

Molecular weight: 22.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5RFD2|A0A1S5RFD2_9CAUD Putative holin OS=Helicobacter phage FrB41M OX=1852663 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84QRR4 pKa = 11.84NEE6 pKa = 3.81TEE8 pKa = 3.38ISFNRR13 pKa = 11.84LRR15 pKa = 11.84EE16 pKa = 3.76ITQAIEE22 pKa = 3.73AQKK25 pKa = 10.86NSEE28 pKa = 4.15KK29 pKa = 10.48TSDD32 pKa = 3.45NANTSEE38 pKa = 4.24ITSQSTPKK46 pKa = 10.32PKK48 pKa = 9.51GTARR52 pKa = 11.84TKK54 pKa = 11.0NSALKK59 pKa = 7.34PTKK62 pKa = 9.79RR63 pKa = 11.84AFSEE67 pKa = 4.16RR68 pKa = 11.84QKK70 pKa = 9.86ITIRR74 pKa = 11.84DD75 pKa = 3.61NSQASAKK82 pKa = 9.47EE83 pKa = 4.04IKK85 pKa = 10.07RR86 pKa = 11.84GRR88 pKa = 3.52

Molecular weight: 9.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36

0

36

9243

37

1571

256.8

29.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.275 ± 0.334

0.833 ± 0.229

4.988 ± 0.308

8.406 ± 0.277

5.182 ± 0.288

4.165 ± 0.294

1.255 ± 0.185

7.281 ± 0.333

11.1 ± 0.459

10.538 ± 0.4

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.937 ± 0.198

8.374 ± 0.387

2.164 ± 0.178

4.414 ± 0.348

4.035 ± 0.239

6.253 ± 0.267

4.425 ± 0.311

4.284 ± 0.35

0.454 ± 0.09

3.635 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski