Amino acid dipepetide frequency for Helicobacter phage FrB41M

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.866AlaAla: 0.866 ± 0.341
0.757AlaCys: 0.757 ± 0.366
2.597AlaAsp: 2.597 ± 0.422
3.246AlaGlu: 3.246 ± 0.641
4.328AlaPhe: 4.328 ± 0.877
2.597AlaGly: 2.597 ± 0.571
1.298AlaHis: 1.298 ± 0.445
5.41AlaIle: 5.41 ± 0.77
7.033AlaLys: 7.033 ± 1.049
10.604AlaLeu: 10.604 ± 1.099
1.515AlaMet: 1.515 ± 0.441
5.843AlaAsn: 5.843 ± 0.809
1.407AlaPro: 1.407 ± 0.496
2.597AlaGln: 2.597 ± 0.466
3.354AlaArg: 3.354 ± 0.592
3.138AlaSer: 3.138 ± 0.533
2.38AlaThr: 2.38 ± 0.782
1.731AlaVal: 1.731 ± 0.478
0.325AlaTrp: 0.325 ± 0.188
1.731AlaTyr: 1.731 ± 0.298
0.0AlaXaa: 0.0 ± 0.0
Cys
0.757CysAla: 0.757 ± 0.381
0.216CysCys: 0.216 ± 0.199
0.866CysAsp: 0.866 ± 0.42
0.866CysGlu: 0.866 ± 0.26
0.649CysPhe: 0.649 ± 0.315
0.433CysGly: 0.433 ± 0.228
0.216CysHis: 0.216 ± 0.156
0.757CysIle: 0.757 ± 0.278
0.433CysLys: 0.433 ± 0.248
0.866CysLeu: 0.866 ± 0.388
0.108CysMet: 0.108 ± 0.109
0.216CysAsn: 0.216 ± 0.165
0.325CysPro: 0.325 ± 0.153
0.108CysGln: 0.108 ± 0.1
0.108CysArg: 0.108 ± 0.104
0.0CysSer: 0.0 ± 0.0
0.649CysThr: 0.649 ± 0.31
0.433CysVal: 0.433 ± 0.246
0.0CysTrp: 0.0 ± 0.0
0.325CysTyr: 0.325 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
3.462AspAla: 3.462 ± 0.496
0.325AspCys: 0.325 ± 0.222
1.839AspAsp: 1.839 ± 0.487
3.571AspGlu: 3.571 ± 0.576
4.977AspPhe: 4.977 ± 0.803
2.272AspGly: 2.272 ± 0.41
0.541AspHis: 0.541 ± 0.275
2.056AspIle: 2.056 ± 0.44
6.384AspLys: 6.384 ± 0.903
6.059AspLeu: 6.059 ± 0.707
1.082AspMet: 1.082 ± 0.26
3.787AspAsn: 3.787 ± 0.633
1.407AspPro: 1.407 ± 0.373
1.082AspGln: 1.082 ± 0.423
1.839AspArg: 1.839 ± 0.38
2.813AspSer: 2.813 ± 0.671
2.164AspThr: 2.164 ± 0.57
0.974AspVal: 0.974 ± 0.362
0.108AspTrp: 0.108 ± 0.118
3.138AspTyr: 3.138 ± 0.602
0.0AspXaa: 0.0 ± 0.0
Glu
5.194GluAla: 5.194 ± 0.821
0.325GluCys: 0.325 ± 0.209
2.056GluAsp: 2.056 ± 0.426
3.787GluGlu: 3.787 ± 0.698
3.354GluPhe: 3.354 ± 0.527
1.948GluGly: 1.948 ± 0.378
1.407GluHis: 1.407 ± 0.504
7.899GluIle: 7.899 ± 1.095
8.981GluLys: 8.981 ± 1.459
10.712GluLeu: 10.712 ± 1.641
1.407GluMet: 1.407 ± 0.407
8.44GluAsn: 8.44 ± 1.064
1.298GluPro: 1.298 ± 0.44
4.653GluGln: 4.653 ± 0.78
4.761GluArg: 4.761 ± 0.582
7.033GluSer: 7.033 ± 0.871
3.895GluThr: 3.895 ± 0.545
4.112GluVal: 4.112 ± 0.818
0.433GluTrp: 0.433 ± 0.204
2.38GluTyr: 2.38 ± 0.452
0.0GluXaa: 0.0 ± 0.0
Phe
2.164PheAla: 2.164 ± 0.493
0.649PheCys: 0.649 ± 0.368
3.246PheAsp: 3.246 ± 0.775
3.354PheGlu: 3.354 ± 0.698
3.03PhePhe: 3.03 ± 0.719
1.298PheGly: 1.298 ± 0.336
0.433PheHis: 0.433 ± 0.162
3.246PheIle: 3.246 ± 0.55
7.682PheLys: 7.682 ± 0.878
7.682PheLeu: 7.682 ± 0.733
0.433PheMet: 0.433 ± 0.249
4.003PheAsn: 4.003 ± 0.779
0.216PhePro: 0.216 ± 0.138
1.407PheGln: 1.407 ± 0.467
1.407PheArg: 1.407 ± 0.274
5.085PheSer: 5.085 ± 0.944
2.164PheThr: 2.164 ± 0.485
2.056PheVal: 2.056 ± 0.465
0.0PheTrp: 0.0 ± 0.0
2.272PheTyr: 2.272 ± 0.502
0.0PheXaa: 0.0 ± 0.0
Gly
3.571GlyAla: 3.571 ± 0.979
0.325GlyCys: 0.325 ± 0.174
1.298GlyAsp: 1.298 ± 0.433
2.38GlyGlu: 2.38 ± 0.473
3.571GlyPhe: 3.571 ± 0.772
2.813GlyGly: 2.813 ± 0.719
0.325GlyHis: 0.325 ± 0.187
2.38GlyIle: 2.38 ± 0.469
3.138GlyLys: 3.138 ± 0.86
4.977GlyLeu: 4.977 ± 0.587
1.515GlyMet: 1.515 ± 0.407
3.03GlyAsn: 3.03 ± 0.474
0.108GlyPro: 0.108 ± 0.103
1.407GlyGln: 1.407 ± 0.355
1.298GlyArg: 1.298 ± 0.462
2.38GlySer: 2.38 ± 0.498
0.974GlyThr: 0.974 ± 0.326
4.112GlyVal: 4.112 ± 0.841
0.216GlyTrp: 0.216 ± 0.174
1.839GlyTyr: 1.839 ± 0.415
0.0GlyXaa: 0.0 ± 0.0
His
0.757HisAla: 0.757 ± 0.291
0.108HisCys: 0.108 ± 0.1
1.19HisAsp: 1.19 ± 0.415
0.866HisGlu: 0.866 ± 0.29
0.433HisPhe: 0.433 ± 0.182
0.325HisGly: 0.325 ± 0.191
0.0HisHis: 0.0 ± 0.0
1.515HisIle: 1.515 ± 0.487
1.298HisLys: 1.298 ± 0.389
1.082HisLeu: 1.082 ± 0.322
0.108HisMet: 0.108 ± 0.117
1.19HisAsn: 1.19 ± 0.339
0.0HisPro: 0.0 ± 0.0
0.108HisGln: 0.108 ± 0.121
0.541HisArg: 0.541 ± 0.169
0.974HisSer: 0.974 ± 0.38
0.757HisThr: 0.757 ± 0.26
0.325HisVal: 0.325 ± 0.182
0.0HisTrp: 0.0 ± 0.0
0.974HisTyr: 0.974 ± 0.254
0.0HisXaa: 0.0 ± 0.0
Ile
4.328IleAla: 4.328 ± 0.878
0.433IleCys: 0.433 ± 0.206
3.895IleAsp: 3.895 ± 0.649
5.085IleGlu: 5.085 ± 0.745
2.921IlePhe: 2.921 ± 0.481
2.272IleGly: 2.272 ± 0.557
0.866IleHis: 0.866 ± 0.298
3.895IleIle: 3.895 ± 0.786
8.332IleLys: 8.332 ± 1.02
8.873IleLeu: 8.873 ± 1.073
1.407IleMet: 1.407 ± 0.385
5.735IleAsn: 5.735 ± 0.751
1.407IlePro: 1.407 ± 0.443
4.328IleGln: 4.328 ± 0.739
3.571IleArg: 3.571 ± 0.534
3.787IleSer: 3.787 ± 0.542
5.302IleThr: 5.302 ± 0.854
3.895IleVal: 3.895 ± 0.619
0.216IleTrp: 0.216 ± 0.154
2.272IleTyr: 2.272 ± 0.53
0.0IleXaa: 0.0 ± 0.0
Lys
8.223LysAla: 8.223 ± 0.897
0.866LysCys: 0.866 ± 0.394
7.358LysAsp: 7.358 ± 1.129
12.768LysGlu: 12.768 ± 1.373
3.787LysPhe: 3.787 ± 0.626
3.138LysGly: 3.138 ± 0.445
2.38LysHis: 2.38 ± 0.612
10.387LysIle: 10.387 ± 1.334
10.928LysLys: 10.928 ± 1.746
6.492LysLeu: 6.492 ± 0.893
1.948LysMet: 1.948 ± 0.423
9.089LysAsn: 9.089 ± 1.028
2.705LysPro: 2.705 ± 0.475
5.951LysGln: 5.951 ± 0.839
4.436LysArg: 4.436 ± 0.769
6.384LysSer: 6.384 ± 0.777
5.194LysThr: 5.194 ± 0.613
5.085LysVal: 5.085 ± 0.873
0.649LysTrp: 0.649 ± 0.275
3.246LysTyr: 3.246 ± 0.431
0.0LysXaa: 0.0 ± 0.0
Leu
4.761LeuAla: 4.761 ± 0.516
1.407LeuCys: 1.407 ± 0.547
4.436LeuAsp: 4.436 ± 0.569
13.633LeuGlu: 13.633 ± 1.568
4.653LeuPhe: 4.653 ± 0.726
5.302LeuGly: 5.302 ± 0.828
0.433LeuHis: 0.433 ± 0.229
6.059LeuIle: 6.059 ± 1.204
16.663LeuLys: 16.663 ± 1.442
6.817LeuLeu: 6.817 ± 0.889
1.407LeuMet: 1.407 ± 0.493
11.686LeuAsn: 11.686 ± 1.334
1.948LeuPro: 1.948 ± 0.438
5.302LeuGln: 5.302 ± 0.979
4.761LeuArg: 4.761 ± 0.838
6.167LeuSer: 6.167 ± 0.989
3.354LeuThr: 3.354 ± 0.591
3.895LeuVal: 3.895 ± 0.565
0.757LeuTrp: 0.757 ± 0.344
1.948LeuTyr: 1.948 ± 0.51
0.0LeuXaa: 0.0 ± 0.0
Met
0.974MetAla: 0.974 ± 0.33
0.108MetCys: 0.108 ± 0.12
1.515MetAsp: 1.515 ± 0.429
0.974MetGlu: 0.974 ± 0.279
0.757MetPhe: 0.757 ± 0.277
1.19MetGly: 1.19 ± 0.415
0.216MetHis: 0.216 ± 0.165
1.515MetIle: 1.515 ± 0.346
1.298MetLys: 1.298 ± 0.408
2.056MetLeu: 2.056 ± 0.375
0.433MetMet: 0.433 ± 0.189
2.056MetAsn: 2.056 ± 0.469
0.757MetPro: 0.757 ± 0.231
1.839MetGln: 1.839 ± 0.503
1.19MetArg: 1.19 ± 0.371
0.757MetSer: 0.757 ± 0.173
0.433MetThr: 0.433 ± 0.245
0.757MetVal: 0.757 ± 0.328
0.108MetTrp: 0.108 ± 0.116
0.433MetTyr: 0.433 ± 0.195
0.0MetXaa: 0.0 ± 0.0
Asn
8.981AsnAla: 8.981 ± 1.213
0.325AsnCys: 0.325 ± 0.196
4.22AsnAsp: 4.22 ± 0.658
8.332AsnGlu: 8.332 ± 1.17
3.462AsnPhe: 3.462 ± 0.609
2.813AsnGly: 2.813 ± 0.396
1.298AsnHis: 1.298 ± 0.391
5.843AsnIle: 5.843 ± 0.964
8.007AsnLys: 8.007 ± 1.088
7.682AsnLeu: 7.682 ± 0.894
1.082AsnMet: 1.082 ± 0.337
6.925AsnAsn: 6.925 ± 1.111
2.164AsnPro: 2.164 ± 0.554
4.653AsnGln: 4.653 ± 0.681
2.272AsnArg: 2.272 ± 0.58
4.328AsnSer: 4.328 ± 0.596
4.653AsnThr: 4.653 ± 0.76
1.948AsnVal: 1.948 ± 0.417
0.433AsnTrp: 0.433 ± 0.193
4.22AsnTyr: 4.22 ± 0.606
0.0AsnXaa: 0.0 ± 0.0
Pro
0.433ProAla: 0.433 ± 0.237
0.108ProCys: 0.108 ± 0.1
0.541ProAsp: 0.541 ± 0.259
1.298ProGlu: 1.298 ± 0.406
1.948ProPhe: 1.948 ± 0.457
0.325ProGly: 0.325 ± 0.226
0.0ProHis: 0.0 ± 0.0
1.731ProIle: 1.731 ± 0.358
2.489ProLys: 2.489 ± 0.527
2.38ProLeu: 2.38 ± 0.632
0.433ProMet: 0.433 ± 0.205
1.839ProAsn: 1.839 ± 0.403
0.866ProPro: 0.866 ± 0.303
0.433ProGln: 0.433 ± 0.258
1.407ProArg: 1.407 ± 0.401
1.839ProSer: 1.839 ± 0.353
1.298ProThr: 1.298 ± 0.324
0.866ProVal: 0.866 ± 0.261
0.0ProTrp: 0.0 ± 0.0
1.407ProTyr: 1.407 ± 0.379
0.0ProXaa: 0.0 ± 0.0
Gln
4.977GlnAla: 4.977 ± 0.822
0.216GlnCys: 0.216 ± 0.218
2.164GlnAsp: 2.164 ± 0.497
4.977GlnGlu: 4.977 ± 0.78
1.623GlnPhe: 1.623 ± 0.383
2.597GlnGly: 2.597 ± 0.486
0.325GlnHis: 0.325 ± 0.209
2.921GlnIle: 2.921 ± 0.595
5.518GlnLys: 5.518 ± 0.731
3.679GlnLeu: 3.679 ± 0.474
1.082GlnMet: 1.082 ± 0.296
3.571GlnAsn: 3.571 ± 0.773
0.757GlnPro: 0.757 ± 0.381
1.19GlnGln: 1.19 ± 0.506
1.731GlnArg: 1.731 ± 0.407
2.272GlnSer: 2.272 ± 0.523
1.515GlnThr: 1.515 ± 0.385
2.164GlnVal: 2.164 ± 0.376
0.108GlnTrp: 0.108 ± 0.116
0.757GlnTyr: 0.757 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
3.03ArgAla: 3.03 ± 0.451
0.216ArgCys: 0.216 ± 0.17
3.03ArgAsp: 3.03 ± 0.521
4.977ArgGlu: 4.977 ± 0.986
2.056ArgPhe: 2.056 ± 0.473
2.164ArgGly: 2.164 ± 0.42
0.866ArgHis: 0.866 ± 0.298
2.272ArgIle: 2.272 ± 0.508
3.895ArgLys: 3.895 ± 0.571
5.41ArgLeu: 5.41 ± 0.893
0.649ArgMet: 0.649 ± 0.312
2.164ArgAsn: 2.164 ± 0.568
0.866ArgPro: 0.866 ± 0.431
1.731ArgGln: 1.731 ± 0.426
0.866ArgArg: 0.866 ± 0.295
1.623ArgSer: 1.623 ± 0.427
1.731ArgThr: 1.731 ± 0.321
1.515ArgVal: 1.515 ± 0.449
0.0ArgTrp: 0.0 ± 0.0
1.298ArgTyr: 1.298 ± 0.434
0.0ArgXaa: 0.0 ± 0.0
Ser
4.328SerAla: 4.328 ± 0.763
0.541SerCys: 0.541 ± 0.295
4.436SerAsp: 4.436 ± 0.511
5.085SerGlu: 5.085 ± 0.879
4.003SerPhe: 4.003 ± 0.75
3.787SerGly: 3.787 ± 0.568
0.541SerHis: 0.541 ± 0.19
4.112SerIle: 4.112 ± 0.709
5.735SerLys: 5.735 ± 0.856
7.25SerLeu: 7.25 ± 1.085
1.407SerMet: 1.407 ± 0.433
3.679SerAsn: 3.679 ± 0.598
1.407SerPro: 1.407 ± 0.389
1.948SerGln: 1.948 ± 0.406
1.839SerArg: 1.839 ± 0.479
3.354SerSer: 3.354 ± 0.642
1.839SerThr: 1.839 ± 0.508
4.003SerVal: 4.003 ± 0.835
0.325SerTrp: 0.325 ± 0.159
2.921SerTyr: 2.921 ± 0.643
0.0SerXaa: 0.0 ± 0.0
Thr
2.38ThrAla: 2.38 ± 0.399
0.325ThrCys: 0.325 ± 0.172
1.731ThrAsp: 1.731 ± 0.567
2.705ThrGlu: 2.705 ± 0.515
0.974ThrPhe: 0.974 ± 0.277
2.38ThrGly: 2.38 ± 0.478
0.541ThrHis: 0.541 ± 0.301
3.895ThrIle: 3.895 ± 0.491
4.544ThrLys: 4.544 ± 0.689
3.571ThrLeu: 3.571 ± 0.626
1.298ThrMet: 1.298 ± 0.31
3.895ThrAsn: 3.895 ± 1.049
2.272ThrPro: 2.272 ± 0.432
3.246ThrGln: 3.246 ± 1.046
1.948ThrArg: 1.948 ± 0.436
3.138ThrSer: 3.138 ± 0.773
3.138ThrThr: 3.138 ± 0.899
0.433ThrVal: 0.433 ± 0.174
0.433ThrTrp: 0.433 ± 0.196
1.407ThrTyr: 1.407 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
1.731ValAla: 1.731 ± 0.486
0.757ValCys: 0.757 ± 0.308
1.731ValAsp: 1.731 ± 0.384
2.705ValGlu: 2.705 ± 0.463
2.597ValPhe: 2.597 ± 0.51
2.597ValGly: 2.597 ± 0.801
0.216ValHis: 0.216 ± 0.151
4.112ValIle: 4.112 ± 0.657
4.653ValLys: 4.653 ± 0.862
5.194ValLeu: 5.194 ± 0.834
0.866ValMet: 0.866 ± 0.349
2.38ValAsn: 2.38 ± 0.451
0.866ValPro: 0.866 ± 0.319
0.649ValGln: 0.649 ± 0.297
1.407ValArg: 1.407 ± 0.457
4.328ValSer: 4.328 ± 0.884
1.298ValThr: 1.298 ± 0.301
3.138ValVal: 3.138 ± 0.806
0.325ValTrp: 0.325 ± 0.144
1.298ValTyr: 1.298 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
0.108TrpAla: 0.108 ± 0.103
0.0TrpCys: 0.0 ± 0.0
0.108TrpAsp: 0.108 ± 0.091
0.649TrpGlu: 0.649 ± 0.352
0.108TrpPhe: 0.108 ± 0.125
0.216TrpGly: 0.216 ± 0.13
0.108TrpHis: 0.108 ± 0.104
0.108TrpIle: 0.108 ± 0.123
0.216TrpLys: 0.216 ± 0.147
0.108TrpLeu: 0.108 ± 0.125
0.216TrpMet: 0.216 ± 0.146
0.757TrpAsn: 0.757 ± 0.358
0.0TrpPro: 0.0 ± 0.0
0.108TrpGln: 0.108 ± 0.125
0.216TrpArg: 0.216 ± 0.144
0.433TrpSer: 0.433 ± 0.251
0.0TrpThr: 0.0 ± 0.0
0.757TrpVal: 0.757 ± 0.317
0.0TrpTrp: 0.0 ± 0.0
0.325TrpTyr: 0.325 ± 0.242
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.731TyrAla: 1.731 ± 0.414
0.325TyrCys: 0.325 ± 0.2
1.623TyrAsp: 1.623 ± 0.401
2.813TyrGlu: 2.813 ± 0.554
2.597TyrPhe: 2.597 ± 0.812
1.19TyrGly: 1.19 ± 0.258
0.541TyrHis: 0.541 ± 0.206
2.705TyrIle: 2.705 ± 0.549
3.787TyrLys: 3.787 ± 0.68
3.895TyrLeu: 3.895 ± 0.932
0.866TyrMet: 0.866 ± 0.312
3.462TyrAsn: 3.462 ± 0.67
0.866TyrPro: 0.866 ± 0.32
1.407TyrGln: 1.407 ± 0.452
1.407TyrArg: 1.407 ± 0.379
2.705TyrSer: 2.705 ± 0.612
1.515TyrThr: 1.515 ± 0.35
0.649TyrVal: 0.649 ± 0.252
0.108TyrTrp: 0.108 ± 0.109
2.164TyrTyr: 2.164 ± 0.545
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (9243 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski