Malvastrum yellow vein Baoshan virus
Average proteome isoelectric point is 7.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D5H3R7|D5H3R7_9GEMI AC4 protein OS=Malvastrum yellow vein Baoshan virus OX=642493 GN=AC4 PE=3 SV=1
MM1 pKa = 7.33 PQPKK5 pKa = 9.9 KK6 pKa = 10.8 FLINAKK12 pKa = 10.13 NYY14 pKa = 8.98 FLTYY18 pKa = 7.3 PHH20 pKa = 6.93 CSISKK25 pKa = 10.47 EE26 pKa = 4.02 EE27 pKa = 4.25 TLSQLQNLNTPTNKK41 pKa = 10.08 KK42 pKa = 9.46 YY43 pKa = 10.83 IKK45 pKa = 9.66 ICRR48 pKa = 11.84 EE49 pKa = 3.67 LHH51 pKa = 6.34 EE52 pKa = 5.83 DD53 pKa = 4.08 GEE55 pKa = 4.49 PHH57 pKa = 5.71 IHH59 pKa = 6.55 VLIQFEE65 pKa = 5.16 GKK67 pKa = 10.07 FKK69 pKa = 10.55 CQNNRR74 pKa = 11.84 FFDD77 pKa = 4.02 LVSPTRR83 pKa = 11.84 SVHH86 pKa = 5.0 FHH88 pKa = 6.81 PNIQGAKK95 pKa = 9.25 SSSDD99 pKa = 3.11 VKK101 pKa = 11.24 SYY103 pKa = 10.79 IDD105 pKa = 3.7 KK106 pKa = 11.33 DD107 pKa = 3.49 GDD109 pKa = 3.67 TLEE112 pKa = 4.14 WGEE115 pKa = 3.84 FQIDD119 pKa = 3.21 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGQQSANDD132 pKa = 4.09 AYY134 pKa = 10.74 AQALNSGSKK143 pKa = 10.2 SEE145 pKa = 4.03 ALNVIRR151 pKa = 11.84 EE152 pKa = 4.21 LAPKK156 pKa = 10.52 DD157 pKa = 3.64 FVLQFHH163 pKa = 6.7 NLNANLDD170 pKa = 4.3 RR171 pKa = 11.84 IFTPPLEE178 pKa = 4.45 VYY180 pKa = 10.31 ISPFSSSSFDD190 pKa = 3.44 QVPDD194 pKa = 3.66 EE195 pKa = 4.36 LEE197 pKa = 3.61 EE198 pKa = 4.11 WACEE202 pKa = 4.06 NVVDD206 pKa = 5.15 AAARR210 pKa = 11.84 PLRR213 pKa = 11.84 PQSIVIEE220 pKa = 4.44 GDD222 pKa = 3.12 SRR224 pKa = 11.84 TGKK227 pKa = 8.52 TMWARR232 pKa = 11.84 SLGPHH237 pKa = 6.71 NYY239 pKa = 10.18 LCGHH243 pKa = 7.38 LDD245 pKa = 4.18 LSPKK249 pKa = 10.15 VYY251 pKa = 11.07 SNDD254 pKa = 2.25 AWYY257 pKa = 10.92 NVIDD261 pKa = 4.79 DD262 pKa = 4.33 VDD264 pKa = 3.73 PHH266 pKa = 6.08 FLKK269 pKa = 10.67 HH270 pKa = 5.9 FKK272 pKa = 10.69 EE273 pKa = 4.43 FMGAQRR279 pKa = 11.84 DD280 pKa = 3.81 WQSNTKK286 pKa = 9.6 YY287 pKa = 10.57 GKK289 pKa = 9.12 PVQIKK294 pKa = 10.41 GGIPTIFLCNPGPNSSYY311 pKa = 11.34 KK312 pKa = 10.62 EE313 pKa = 3.85 FLDD316 pKa = 3.97 EE317 pKa = 4.83 EE318 pKa = 4.8 KK319 pKa = 10.25 NTALKK324 pKa = 10.55 NWALKK329 pKa = 10.24 NAIFVTLEE337 pKa = 3.93 GPLYY341 pKa = 10.74 SGSNQSTAQGSEE353 pKa = 3.84 EE354 pKa = 4.11 TQQEE358 pKa = 4.32 EE359 pKa = 4.54 EE360 pKa = 4.33 SRR362 pKa = 11.84 SS363 pKa = 3.6
Molecular weight: 41.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.5
IPC2_protein 5.474
IPC_protein 5.474
Toseland 5.792
ProMoST 5.753
Dawson 5.652
Bjellqvist 5.703
Wikipedia 5.626
Rodwell 5.626
Grimsley 5.906
Solomon 5.652
Lehninger 5.639
Nozaki 5.893
DTASelect 6.071
Thurlkill 6.008
EMBOSS 5.97
Sillero 5.982
Patrickios 4.088
IPC_peptide 5.677
IPC2_peptide 5.982
IPC2.peptide.svr19 6.004
Protein with the highest isoelectric point:
>tr|D5H3R4|D5H3R4_9GEMI Replication enhancer OS=Malvastrum yellow vein Baoshan virus OX=642493 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.52 IVISTPASKK16 pKa = 10.29 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 3.18 TPYY27 pKa = 7.99 STRR30 pKa = 11.84 VAVPTVRR37 pKa = 11.84 VTKK40 pKa = 10.7 SRR42 pKa = 11.84 MWANRR47 pKa = 11.84 PTNRR51 pKa = 11.84 KK52 pKa = 7.45 PRR54 pKa = 11.84 MYY56 pKa = 10.75 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.02 SRR80 pKa = 11.84 HH81 pKa = 5.54 DD82 pKa = 3.51 VVHH85 pKa = 6.75 IGKK88 pKa = 9.41 VMCISDD94 pKa = 3.67 VTRR97 pKa = 11.84 GTGLTHH103 pKa = 7.09 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.39 EE124 pKa = 4.08 NIKK127 pKa = 10.76 SKK129 pKa = 10.6 NHH131 pKa = 5.54 TNNVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.88 RR143 pKa = 11.84 RR144 pKa = 11.84 PTGTPQDD151 pKa = 3.8 FGDD154 pKa = 3.96 VFNMFDD160 pKa = 4.34 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.77 QVLRR180 pKa = 11.84 KK181 pKa = 9.41 WYY183 pKa = 8.08 ATVTGGQYY191 pKa = 9.96 GAKK194 pKa = 9.62 EE195 pKa = 3.57 QALVKK200 pKa = 10.72 KK201 pKa = 9.81 FVKK204 pKa = 9.9 VNNYY208 pKa = 7.85 VVYY211 pKa = 10.11 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.08 YY219 pKa = 8.01 EE220 pKa = 4.07 NHH222 pKa = 6.29 SEE224 pKa = 4.11 NALMLYY230 pKa = 7.58 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.64 FYY251 pKa = 11.34 DD252 pKa = 3.32 SVTNN256 pKa = 3.98
Molecular weight: 29.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.264
IPC2_protein 9.56
IPC_protein 9.765
Toseland 10.116
ProMoST 9.853
Dawson 10.335
Bjellqvist 10.028
Wikipedia 10.526
Rodwell 10.687
Grimsley 10.409
Solomon 10.365
Lehninger 10.321
Nozaki 10.145
DTASelect 10.028
Thurlkill 10.175
EMBOSS 10.526
Sillero 10.248
Patrickios 10.204
IPC_peptide 10.365
IPC2_peptide 8.887
IPC2.peptide.svr19 8.522
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1114
96
363
185.7
21.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.399 ± 0.618
2.244 ± 0.508
4.668 ± 0.6
5.117 ± 0.952
4.309 ± 0.356
5.117 ± 0.324
3.591 ± 0.555
5.117 ± 1.152
5.745 ± 0.702
7.092 ± 1.006
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.154 ± 0.617
6.463 ± 0.29
6.194 ± 0.414
4.578 ± 0.618
6.373 ± 1.103
9.425 ± 1.606
5.925 ± 1.602
6.194 ± 1.455
1.526 ± 0.242
3.77 ± 0.618
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here