Sphingosinicella microcystinivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingosinicellaceae; Sphingosinicella

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495RWP5|A0A495RWP5_SPHMI Dihydrolipoyl dehydrogenase OS=Sphingosinicella microcystinivorans OX=335406 GN=DFR51_1562 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.39KK3 pKa = 10.3GIRR6 pKa = 11.84SRR8 pKa = 11.84AISTTVLAAATLVGSLAVAAPAFAQDD34 pKa = 4.73AEE36 pKa = 4.44ATSDD40 pKa = 3.38WEE42 pKa = 3.9ISGNAGIFSQYY53 pKa = 7.31RR54 pKa = 11.84WRR56 pKa = 11.84GISLSDD62 pKa = 3.63EE63 pKa = 3.98DD64 pKa = 4.16VAFQGGIDD72 pKa = 3.76AAHH75 pKa = 6.47SSGFYY80 pKa = 9.94VGTWGSSLAGYY91 pKa = 10.1GSYY94 pKa = 10.66GGSNTEE100 pKa = 3.29IDD102 pKa = 4.04VYY104 pKa = 11.18AGYY107 pKa = 10.61AGEE110 pKa = 4.68AGGFGYY116 pKa = 10.44DD117 pKa = 5.08IGAIWYY123 pKa = 7.2LYY125 pKa = 9.63PGTSGTDD132 pKa = 3.26VVEE135 pKa = 4.5ITASLSKK142 pKa = 10.61EE143 pKa = 4.04LGPVGASVGVAYY155 pKa = 10.16APKK158 pKa = 9.65QDD160 pKa = 4.27SLSSDD165 pKa = 3.3DD166 pKa = 4.88SFYY169 pKa = 11.24LYY171 pKa = 10.19TDD173 pKa = 3.63WSAAIPNTPVSLNAHH188 pKa = 6.94LGYY191 pKa = 9.41TDD193 pKa = 3.95GSLSIDD199 pKa = 3.3RR200 pKa = 11.84GFSTDD205 pKa = 3.51GDD207 pKa = 4.04YY208 pKa = 11.55LDD210 pKa = 3.98WSLGASVSYY219 pKa = 11.08DD220 pKa = 3.13KK221 pKa = 10.88LTLGVSYY228 pKa = 11.3VDD230 pKa = 3.53TDD232 pKa = 3.79VKK234 pKa = 11.25GGDD237 pKa = 3.43PSINKK242 pKa = 8.26ITDD245 pKa = 3.13AAIIVSLTAAFF256 pKa = 3.92

Molecular weight: 26.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495RDJ9|A0A495RDJ9_SPHMI tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Sphingosinicella microcystinivorans OX=335406 GN=mnmG PE=3 SV=1
MM1 pKa = 7.63KK2 pKa = 9.74PPPHH6 pKa = 6.84RR7 pKa = 11.84ASDD10 pKa = 3.47PGLRR14 pKa = 11.84RR15 pKa = 11.84QQFGHH20 pKa = 5.7VVWVHH25 pKa = 6.47CPQCDD30 pKa = 3.76GPAKK34 pKa = 10.5HH35 pKa = 6.27NALGVKK41 pKa = 9.81CIRR44 pKa = 11.84CGYY47 pKa = 6.57MTIPVIKK54 pKa = 9.49PAAVQWARR62 pKa = 11.84ITISDD67 pKa = 4.5PRR69 pKa = 11.84CSHH72 pKa = 6.45CRR74 pKa = 11.84NPLPDD79 pKa = 3.3TSRR82 pKa = 11.84PLARR86 pKa = 11.84GGDD89 pKa = 3.7EE90 pKa = 3.77NRR92 pKa = 11.84PVVRR96 pKa = 11.84VRR98 pKa = 11.84CPHH101 pKa = 6.24CAKK104 pKa = 10.15TMAYY108 pKa = 8.24PARR111 pKa = 11.84AWSAPSRR118 pKa = 11.84ASQQQPRR125 pKa = 11.84RR126 pKa = 11.84LKK128 pKa = 10.34PYY130 pKa = 9.07LTAQVAGNLLMVDD143 pKa = 3.97NLAHH147 pKa = 6.94LSALEE152 pKa = 4.34TYY154 pKa = 10.53LGAALRR160 pKa = 11.84EE161 pKa = 4.07RR162 pKa = 11.84GPVRR166 pKa = 11.84GLTMMARR173 pKa = 11.84LPAWMKK179 pKa = 10.74SSTNRR184 pKa = 11.84AKK186 pKa = 10.47ILRR189 pKa = 11.84SLRR192 pKa = 11.84QLADD196 pKa = 3.55RR197 pKa = 11.84ASKK200 pKa = 10.87DD201 pKa = 3.8GIDD204 pKa = 3.58EE205 pKa = 4.14

Molecular weight: 22.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3645

0

3645

1206989

25

3982

331.1

35.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.27 ± 0.061

0.785 ± 0.013

5.866 ± 0.029

5.582 ± 0.036

3.69 ± 0.025

8.964 ± 0.052

1.962 ± 0.02

5.044 ± 0.026

3.124 ± 0.033

9.757 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.022

2.575 ± 0.031

5.178 ± 0.033

2.759 ± 0.022

7.237 ± 0.045

5.297 ± 0.032

5.424 ± 0.037

7.355 ± 0.028

1.387 ± 0.017

2.34 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski