Petrocella atlantisensis
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3381 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3P7PT94|A0A3P7PT94_9FIRM Aminotransferase OS=Petrocella atlantisensis OX=2173034 GN=dapL PE=3 SV=1
MM1 pKa = 8.04 SIGTQDD7 pKa = 3.29 KK8 pKa = 10.74 VVTISAATNANYY20 pKa = 10.05 TEE22 pKa = 4.57 ILRR25 pKa = 11.84 QVLNASYY32 pKa = 11.43 NEE34 pKa = 3.79 FDD36 pKa = 3.5 TTYY39 pKa = 10.88 DD40 pKa = 3.23 AYY42 pKa = 10.01 IANVDD47 pKa = 3.59 AGSGAFLLDD56 pKa = 3.25 SLAIFLYY63 pKa = 10.64 ADD65 pKa = 3.42 QADD68 pKa = 3.99 LSGTLYY74 pKa = 11.39 ANMTLSDD81 pKa = 4.0 SEE83 pKa = 5.14 GNGNTVWSDD92 pKa = 3.49 AEE94 pKa = 4.07 KK95 pKa = 11.02 KK96 pKa = 9.53 EE97 pKa = 3.98 ALRR100 pKa = 11.84 EE101 pKa = 3.94 ALEE104 pKa = 4.13 ITKK107 pKa = 10.68 NNYY110 pKa = 9.5 NNLAEE115 pKa = 4.24 VQADD119 pKa = 3.62 LTTIAEE125 pKa = 4.2 RR126 pKa = 11.84 VDD128 pKa = 3.99 FTTILYY134 pKa = 10.06 DD135 pKa = 3.4 GQQYY139 pKa = 10.71 KK140 pKa = 10.45 SVVVTNSQDD149 pKa = 3.44 VEE151 pKa = 4.31 VASYY155 pKa = 10.45 IQGGDD160 pKa = 2.92 KK161 pKa = 10.71 AGYY164 pKa = 9.94 IEE166 pKa = 5.67 DD167 pKa = 4.34 LFNEE171 pKa = 4.56 LEE173 pKa = 4.16 ALTMSSNVSYY183 pKa = 9.92 TVTTTLADD191 pKa = 3.58 DD192 pKa = 4.1 TARR195 pKa = 11.84 TATFGANN202 pKa = 3.03
Molecular weight: 21.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.884
DTASelect 4.101
Thurlkill 3.617
EMBOSS 3.706
Sillero 3.897
Patrickios 1.036
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A3P7NYE3|A0A3P7NYE3_9FIRM Pseudouridine-5'-phosphate glycosidase OS=Petrocella atlantisensis OX=2173034 GN=yeiN PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 8.76 KK9 pKa = 7.46 RR10 pKa = 11.84 QRR12 pKa = 11.84 SRR14 pKa = 11.84 EE15 pKa = 3.65 HH16 pKa = 6.42 GFRR19 pKa = 11.84 KK20 pKa = 9.76 RR21 pKa = 11.84 MKK23 pKa = 9.67 NKK25 pKa = 9.89 SGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.2 GRR39 pKa = 11.84 KK40 pKa = 8.14 QLSAA44 pKa = 3.9
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.337
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.223
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.959
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.025
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3381
0
3381
1030731
22
2474
304.9
34.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.397 ± 0.039
0.904 ± 0.015
5.855 ± 0.031
7.324 ± 0.044
4.127 ± 0.029
6.523 ± 0.041
2.066 ± 0.024
8.944 ± 0.042
7.266 ± 0.037
9.509 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.206 ± 0.021
4.749 ± 0.031
3.05 ± 0.024
3.127 ± 0.022
3.827 ± 0.031
5.949 ± 0.035
5.481 ± 0.038
6.848 ± 0.037
0.75 ± 0.012
4.1 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here