Coriobacteriales bacterium OH1046
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2118 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A437UUM9|A0A437UUM9_9ACTN DNA topoisomerase 1 OS=Coriobacteriales bacterium OH1046 OX=2491116 GN=topA PE=3 SV=1
MM1 pKa = 7.14 KK2 pKa = 10.2 HH3 pKa = 6.14 LRR5 pKa = 11.84 TGIAACAVAVALAVGGCTGQADD27 pKa = 4.14 EE28 pKa = 4.75 TTSGTAEE35 pKa = 3.85 QGTALDD41 pKa = 3.71 QAGEE45 pKa = 4.38 TEE47 pKa = 4.48 RR48 pKa = 11.84 SAEE51 pKa = 4.13 EE52 pKa = 4.17 PFSLTRR58 pKa = 11.84 DD59 pKa = 3.44 EE60 pKa = 5.11 LVIDD64 pKa = 3.69 EE65 pKa = 5.49 TITDD69 pKa = 4.14 DD70 pKa = 4.34 ADD72 pKa = 3.36 EE73 pKa = 4.25 SHH75 pKa = 7.21 AIIADD80 pKa = 3.7 GTTEE84 pKa = 4.06 SYY86 pKa = 11.02 RR87 pKa = 11.84 SIEE90 pKa = 4.11 VVKK93 pKa = 10.2 TGDD96 pKa = 3.57 ADD98 pKa = 3.82 GDD100 pKa = 3.94 GADD103 pKa = 4.3 FYY105 pKa = 10.88 GTNAAVLATNGASLDD120 pKa = 3.9 LADD123 pKa = 5.86 SIVTSDD129 pKa = 3.4 GTHH132 pKa = 6.29 ANAVFSYY139 pKa = 11.54 GEE141 pKa = 4.14 GTVVNIAHH149 pKa = 6.8 SIIEE153 pKa = 4.37 TTGDD157 pKa = 3.4 CSGGIMVTGGGTLNATDD174 pKa = 5.22 LYY176 pKa = 11.54 VHH178 pKa = 5.82 TTGNSSAAIRR188 pKa = 11.84 SDD190 pKa = 3.03 RR191 pKa = 11.84 GGGTEE196 pKa = 4.19 NISGGTYY203 pKa = 8.13 ITDD206 pKa = 5.21 GIGSPVIYY214 pKa = 9.34 STADD218 pKa = 3.21 VTVSDD223 pKa = 4.1 ATLEE227 pKa = 4.33 STASQGVVVEE237 pKa = 4.74 GKK239 pKa = 10.46 NSVTLVDD246 pKa = 4.37 CNLIASNTAKK256 pKa = 10.77 NSDD259 pKa = 3.25 KK260 pKa = 11.1 SDD262 pKa = 2.84 WYY264 pKa = 8.91 QAVMIYY270 pKa = 10.81 QSMSGDD276 pKa = 3.54 AAEE279 pKa = 4.94 GEE281 pKa = 4.47 AFFTMEE287 pKa = 4.51 GAASSIGTATFSSS300 pKa = 3.97
Molecular weight: 30.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.834
IPC_protein 3.834
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.668
Grimsley 3.528
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.164
Thurlkill 3.668
EMBOSS 3.757
Sillero 3.961
Patrickios 1.888
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.854
Protein with the highest isoelectric point:
>tr|A0A437UTP7|A0A437UTP7_9ACTN LacI family transcriptional regulator OS=Coriobacteriales bacterium OH1046 OX=2491116 GN=EII22_08715 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.11 GGRR28 pKa = 11.84 AVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.11 GRR39 pKa = 11.84 KK40 pKa = 8.89 SLTVV44 pKa = 3.12
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2118
0
2118
691650
33
2867
326.6
35.5
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.508 ± 0.068
1.61 ± 0.022
6.253 ± 0.04
6.5 ± 0.049
3.686 ± 0.031
8.676 ± 0.047
1.974 ± 0.024
5.859 ± 0.045
3.253 ± 0.037
9.576 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.714 ± 0.028
2.589 ± 0.03
4.293 ± 0.03
2.537 ± 0.022
6.387 ± 0.055
5.982 ± 0.044
5.093 ± 0.06
7.473 ± 0.038
1.087 ± 0.019
2.951 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here