Novosphingobium pentaromativorans US6-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium; Novosphingobium pentaromativorans

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G6EJ98|G6EJ98_9SPHN Uncharacterized protein OS=Novosphingobium pentaromativorans US6-1 OX=1088721 GN=NSU_4419 PE=4 SV=1
MM1 pKa = 7.59PPAGATDD8 pKa = 3.51SFVYY12 pKa = 10.13TITDD16 pKa = 3.32GDD18 pKa = 4.4GDD20 pKa = 4.04TSTTTLTISLTDD32 pKa = 3.29SGLAASNEE40 pKa = 4.06DD41 pKa = 3.37ASVDD45 pKa = 3.65EE46 pKa = 4.54AALGIGSNPSSTAEE60 pKa = 4.18TVSGSLTDD68 pKa = 3.71NASGGAGGYY77 pKa = 8.45TYY79 pKa = 11.42ALVGSATGSHH89 pKa = 5.41GTITIDD95 pKa = 4.22PSGSWSYY102 pKa = 10.1TLTSPVDD109 pKa = 3.46GATLDD114 pKa = 3.67NGVTTEE120 pKa = 4.07NNLEE124 pKa = 4.11SFTYY128 pKa = 10.19QVTDD132 pKa = 3.57ANGNTTTSTITIDD145 pKa = 4.24VIDD148 pKa = 4.92DD149 pKa = 3.77VPTARR154 pKa = 11.84ADD156 pKa = 3.44TDD158 pKa = 4.12SVVEE162 pKa = 4.23GASTDD167 pKa = 3.66GNVLSGTGTTSGLVDD182 pKa = 3.27VLGADD187 pKa = 4.26GAAPGGAVTGVATGTDD203 pKa = 3.06TSAPVTGNLGGAGIAGTYY221 pKa = 7.35GTLILNADD229 pKa = 3.47GSYY232 pKa = 10.12TYY234 pKa = 11.23NSTANSISGNATDD247 pKa = 3.85SFVYY251 pKa = 10.1TITDD255 pKa = 3.32GDD257 pKa = 4.4GDD259 pKa = 4.04TSTTTLTISLTDD271 pKa = 3.38VSLVGDD277 pKa = 4.18DD278 pKa = 4.11EE279 pKa = 5.01SVTVYY284 pKa = 10.67EE285 pKa = 4.63KK286 pKa = 11.15ALDD289 pKa = 3.92TAQTGADD296 pKa = 3.48VAAGSITGSMPSDD309 pKa = 3.77PGEE312 pKa = 4.14TASGAVSVTGATGYY326 pKa = 10.47SIAGGSVSGNEE337 pKa = 3.95TTVVGTYY344 pKa = 7.73GTLVLNNSTGAFTYY358 pKa = 10.21TLTSPVTTTPSANDD372 pKa = 3.73GVTTALGAEE381 pKa = 4.4SFTYY385 pKa = 9.15TASDD389 pKa = 3.46ANGNTTTGTISIDD402 pKa = 3.74VVDD405 pKa = 4.38DD406 pKa = 3.45TPYY409 pKa = 11.28AVVPEE414 pKa = 5.18AIQVTNGAAPVSAPADD430 pKa = 3.73LDD432 pKa = 3.82ADD434 pKa = 3.57MSVIDD439 pKa = 4.68NYY441 pKa = 11.23GADD444 pKa = 3.41GAGTVRR450 pKa = 11.84FAPSLDD456 pKa = 3.71GADD459 pKa = 3.99SGLTHH464 pKa = 6.73NFVSITYY471 pKa = 8.28TLVSDD476 pKa = 4.52TVLEE480 pKa = 4.7AYY482 pKa = 10.22AGADD486 pKa = 3.34KK487 pKa = 10.89IFTVTLDD494 pKa = 3.71PTTATYY500 pKa = 9.29TVDD503 pKa = 3.04MDD505 pKa = 3.79GTIDD509 pKa = 4.17SIQTIDD515 pKa = 4.01FNAGGYY521 pKa = 10.3NFVGGNNSWSGFIPVGEE538 pKa = 4.46TVGSPIDD545 pKa = 4.07NNSQDD550 pKa = 4.57LLLTPSINNADD561 pKa = 4.18DD562 pKa = 3.65GTINSTANTGGIGGGASVGSNEE584 pKa = 3.97TFRR587 pKa = 11.84VDD589 pKa = 4.03FVTDD593 pKa = 3.55LRR595 pKa = 11.84GDD597 pKa = 3.87PKK599 pKa = 10.96DD600 pKa = 3.49GAGDD604 pKa = 3.9YY605 pKa = 9.02DD606 pKa = 3.92TDD608 pKa = 3.76TNRR611 pKa = 11.84DD612 pKa = 3.16HH613 pKa = 6.91MFDD616 pKa = 2.56GHH618 pKa = 5.09YY619 pKa = 9.32TVNGAVALFKK629 pKa = 10.17STNGSTVNIAAFDD642 pKa = 4.31DD643 pKa = 4.45PDD645 pKa = 4.72GNTAVGDD652 pKa = 3.84GDD654 pKa = 3.87KK655 pKa = 11.31DD656 pKa = 4.32SITGISIVWRR666 pKa = 11.84GTQYY670 pKa = 11.24VDD672 pKa = 3.7GSGDD676 pKa = 3.93PIIIPTITATNYY688 pKa = 7.78TVNGHH693 pKa = 5.33VFTVTLLGDD702 pKa = 3.77GSVNVAGVEE711 pKa = 3.98GDD713 pKa = 3.65PGSSQVGTQIAVFTGDD729 pKa = 3.17GFNSVEE735 pKa = 4.12YY736 pKa = 8.82TWAGGDD742 pKa = 3.44TFQIGDD748 pKa = 4.39FGASTLTNDD757 pKa = 4.1PVNFTIPVEE766 pKa = 4.28VVDD769 pKa = 4.37GDD771 pKa = 4.23GDD773 pKa = 3.72VSAQSNLSITAVTTTPPVALDD794 pKa = 3.64LDD796 pKa = 4.22GDD798 pKa = 4.09GVEE801 pKa = 5.61FVGLDD806 pKa = 3.01AGVTHH811 pKa = 7.96DD812 pKa = 4.2YY813 pKa = 11.45GSGLVQTAWLSADD826 pKa = 4.26DD827 pKa = 4.93GLLAHH832 pKa = 6.82DD833 pKa = 4.71TGHH836 pKa = 6.61GLDD839 pKa = 4.6IVFTDD844 pKa = 4.2DD845 pKa = 3.49AAGAEE850 pKa = 4.18TDD852 pKa = 4.38LEE854 pKa = 4.31GLRR857 pKa = 11.84LAYY860 pKa = 10.2DD861 pKa = 3.57SNGDD865 pKa = 3.67GKK867 pKa = 9.48LTAADD872 pKa = 3.59EE873 pKa = 4.72SYY875 pKa = 11.34SEE877 pKa = 4.12FGVWQDD883 pKa = 3.25ANSNGVVDD891 pKa = 4.29AGEE894 pKa = 4.36FKK896 pKa = 10.43TLAQMGITSIEE907 pKa = 4.06LTAEE911 pKa = 4.64GPGSSQADD919 pKa = 3.1GDD921 pKa = 4.83AIVHH925 pKa = 5.46ATGEE929 pKa = 4.28YY930 pKa = 8.18TMKK933 pKa = 10.67GATYY937 pKa = 10.98ALADD941 pKa = 3.59VSFATASVEE950 pKa = 4.22SQTAARR956 pKa = 11.84TAEE959 pKa = 4.02MAAITAAAAGFMMSGAAHH977 pKa = 7.38AMPLSPIAIEE987 pKa = 4.77AIAAAMQTFEE997 pKa = 4.05THH999 pKa = 5.79LPEE1002 pKa = 4.01VTVAVPEE1009 pKa = 4.32HH1010 pKa = 6.75APATPPVTFLQDD1022 pKa = 3.15GQQAHH1027 pKa = 5.91QDD1029 pKa = 3.98SEE1031 pKa = 4.78PEE1033 pKa = 4.08SHH1035 pKa = 7.36PSQHH1039 pKa = 6.62AAEE1042 pKa = 4.28QGAGHH1047 pKa = 6.82GLGDD1051 pKa = 3.82VVQHH1055 pKa = 6.05QDD1057 pKa = 3.33VSVQPLGSGEE1067 pKa = 4.13EE1068 pKa = 4.22GHH1070 pKa = 6.28VQASAAPALFGGADD1084 pKa = 3.55SGLMQALLLAAQGEE1098 pKa = 4.52QAGGSKK1104 pKa = 10.58DD1105 pKa = 3.38QGNGTGAGQGSGHH1118 pKa = 6.27GGEE1121 pKa = 4.72HH1122 pKa = 7.14GSGNLPAVQEE1132 pKa = 4.86AIAEE1136 pKa = 4.6SAGHH1140 pKa = 6.27HH1141 pKa = 6.13AVDD1144 pKa = 4.37SLIDD1148 pKa = 3.59HH1149 pKa = 7.02FAGHH1153 pKa = 5.99QGAHH1157 pKa = 6.43GAPAIAEE1164 pKa = 4.22TMGADD1169 pKa = 3.85GLDD1172 pKa = 3.55HH1173 pKa = 6.9VLGAMLAGGGLDD1185 pKa = 3.65GLHH1188 pKa = 6.73AAGLAGGFEE1197 pKa = 4.44MAAMAAAMEE1206 pKa = 4.24AHH1208 pKa = 6.04STTVAA1213 pKa = 3.19

Molecular weight: 120.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G6EAJ4|G6EAJ4_9SPHN Flagellar hook-associated protein 1 OS=Novosphingobium pentaromativorans US6-1 OX=1088721 GN=flgK PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.44GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 8.11VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5229

0

5229

1570680

35

4258

300.4

32.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.561 ± 0.05

0.932 ± 0.011

5.928 ± 0.022

5.924 ± 0.033

3.605 ± 0.022

8.687 ± 0.036

2.077 ± 0.019

5.048 ± 0.023

3.14 ± 0.026

9.84 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.016

2.588 ± 0.022

5.191 ± 0.024

3.204 ± 0.021

7.296 ± 0.041

5.646 ± 0.025

5.058 ± 0.027

7.063 ± 0.027

1.425 ± 0.015

2.227 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski