Microbacterium mangrovi

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3509 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B2A9Z8|A0A0B2A9Z8_9MICO ATP-binding protein OS=Microbacterium mangrovi OX=1348253 GN=LK09_05575 PE=4 SV=1
MM1 pKa = 7.79TDD3 pKa = 3.81LTALSYY9 pKa = 9.57TAEE12 pKa = 4.41HH13 pKa = 6.44EE14 pKa = 4.51WVATGADD21 pKa = 3.51VVTIGITDD29 pKa = 4.22FAADD33 pKa = 3.42KK34 pKa = 10.87LGDD37 pKa = 3.73VVYY40 pKa = 11.18VDD42 pKa = 4.87LPAVGTEE49 pKa = 3.75VTAAEE54 pKa = 4.2VCGEE58 pKa = 3.99IEE60 pKa = 4.18STKK63 pKa = 10.84SVGEE67 pKa = 4.02LYY69 pKa = 10.87APVTGTVVEE78 pKa = 4.37INDD81 pKa = 4.02AVVDD85 pKa = 4.35DD86 pKa = 4.65PSLVNAEE93 pKa = 4.09PFEE96 pKa = 4.56GGWLLRR102 pKa = 11.84IQVAAGATDD111 pKa = 3.68GLLDD115 pKa = 3.72RR116 pKa = 11.84AAYY119 pKa = 7.73EE120 pKa = 3.97ALTGGEE126 pKa = 4.13AA127 pKa = 3.63

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B2A0W5|A0A0B2A0W5_9MICO 50S ribosomal protein L29 OS=Microbacterium mangrovi OX=1348253 GN=rpmC PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 10.04KK16 pKa = 8.86HH17 pKa = 4.36GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILAARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.31GRR40 pKa = 11.84SEE42 pKa = 4.21LSAA45 pKa = 4.73

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3509

0

3509

1144491

37

12100

326.2

34.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.9 ± 0.064

0.521 ± 0.009

6.22 ± 0.036

5.089 ± 0.064

3.182 ± 0.025

9.057 ± 0.073

2.052 ± 0.023

4.595 ± 0.033

1.929 ± 0.029

10.017 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.835 ± 0.022

1.931 ± 0.023

5.446 ± 0.028

2.852 ± 0.023

7.202 ± 0.069

5.398 ± 0.035

6.146 ± 0.088

9.104 ± 0.038

1.531 ± 0.022

1.994 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski