Amino acid dipepetide frequency for Fibrobacter sp. UWEL

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.429AlaAla: 8.429 ± 0.158	1.213AlaCys: 1.213 ± 0.036	4.903AlaAsp: 4.903 ± 0.07	5.892AlaGlu: 5.892 ± 0.103	3.691AlaPhe: 3.691 ± 0.068	6.605AlaGly: 6.605 ± 0.1	1.248AlaHis: 1.248 ± 0.033	4.873AlaIle: 4.873 ± 0.077	5.646AlaLys: 5.646 ± 0.085	7.701AlaLeu: 7.701 ± 0.097	2.274AlaMet: 2.274 ± 0.056	3.469AlaAsn: 3.469 ± 0.064	3.251AlaPro: 3.251 ± 0.082	2.724AlaGln: 2.724 ± 0.058	3.353AlaArg: 3.353 ± 0.067	5.233AlaSer: 5.233 ± 0.094	4.288AlaThr: 4.288 ± 0.073	5.888AlaVal: 5.888 ± 0.075	0.841AlaTrp: 0.841 ± 0.035	2.683AlaTyr: 2.683 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.065CysAla: 1.065 ± 0.034	0.232CysCys: 0.232 ± 0.016	0.859CysAsp: 0.859 ± 0.035	0.849CysGlu: 0.849 ± 0.029	0.57CysPhe: 0.57 ± 0.023	1.263CysGly: 1.263 ± 0.04	0.271CysHis: 0.271 ± 0.017	0.696CysIle: 0.696 ± 0.025	0.853CysLys: 0.853 ± 0.031	1.05CysLeu: 1.05 ± 0.032	0.294CysMet: 0.294 ± 0.017	0.575CysAsn: 0.575 ± 0.024	0.627CysPro: 0.627 ± 0.031	0.324CysGln: 0.324 ± 0.017	0.571CysArg: 0.571 ± 0.024	0.914CysSer: 0.914 ± 0.03	0.701CysThr: 0.701 ± 0.031	0.952CysVal: 0.952 ± 0.032	0.123CysTrp: 0.123 ± 0.011	0.545CysTyr: 0.545 ± 0.025	0.0CysXaa: 0.0 ± 0.0
Asp	4.812AspAla: 4.812 ± 0.075	0.745AspCys: 0.745 ± 0.029	3.512AspAsp: 3.512 ± 0.076	4.317AspGlu: 4.317 ± 0.068	3.199AspPhe: 3.199 ± 0.054	4.974AspGly: 4.974 ± 0.106	1.01AspHis: 1.01 ± 0.032	3.529AspIle: 3.529 ± 0.071	3.367AspLys: 3.367 ± 0.062	5.018AspLeu: 5.018 ± 0.074	1.55AspMet: 1.55 ± 0.039	2.473AspAsn: 2.473 ± 0.047	2.442AspPro: 2.442 ± 0.059	1.493AspGln: 1.493 ± 0.041	2.455AspArg: 2.455 ± 0.061	4.582AspSer: 4.582 ± 0.094	3.022AspThr: 3.022 ± 0.073	4.27AspVal: 4.27 ± 0.067	0.841AspTrp: 0.841 ± 0.034	2.625AspTyr: 2.625 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.084GluAla: 5.084 ± 0.09	0.746GluCys: 0.746 ± 0.027	3.607GluAsp: 3.607 ± 0.065	4.582GluGlu: 4.582 ± 0.106	2.885GluPhe: 2.885 ± 0.053	4.258GluGly: 4.258 ± 0.066	1.146GluHis: 1.146 ± 0.035	4.284GluIle: 4.284 ± 0.059	5.178GluLys: 5.178 ± 0.086	5.556GluLeu: 5.556 ± 0.083	2.032GluMet: 2.032 ± 0.044	3.851GluAsn: 3.851 ± 0.064	1.965GluPro: 1.965 ± 0.052	2.088GluGln: 2.088 ± 0.044	2.963GluArg: 2.963 ± 0.064	4.026GluSer: 4.026 ± 0.072	3.492GluThr: 3.492 ± 0.066	4.111GluVal: 4.111 ± 0.062	0.843GluTrp: 0.843 ± 0.027	2.37GluTyr: 2.37 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	4.05PheAla: 4.05 ± 0.062	0.786PheCys: 0.786 ± 0.032	3.124PheAsp: 3.124 ± 0.059	2.962PheGlu: 2.962 ± 0.052	2.33PhePhe: 2.33 ± 0.059	3.71PheGly: 3.71 ± 0.065	0.805PheHis: 0.805 ± 0.029	2.532PheIle: 2.532 ± 0.056	2.793PheLys: 2.793 ± 0.054	4.025PheLeu: 4.025 ± 0.078	1.157PheMet: 1.157 ± 0.037	2.056PheAsn: 2.056 ± 0.046	1.698PhePro: 1.698 ± 0.041	1.329PheGln: 1.329 ± 0.031	2.055PheArg: 2.055 ± 0.044	3.24PheSer: 3.24 ± 0.063	2.535PheThr: 2.535 ± 0.054	3.406PheVal: 3.406 ± 0.068	0.668PheTrp: 0.668 ± 0.028	1.765PheTyr: 1.765 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	5.515GlyAla: 5.515 ± 0.083	1.159GlyCys: 1.159 ± 0.033	4.315GlyAsp: 4.315 ± 0.098	4.557GlyGlu: 4.557 ± 0.071	3.707GlyPhe: 3.707 ± 0.068	5.289GlyGly: 5.289 ± 0.101	1.3GlyHis: 1.3 ± 0.037	4.717GlyIle: 4.717 ± 0.079	5.443GlyLys: 5.443 ± 0.068	6.18GlyLeu: 6.18 ± 0.085	2.048GlyMet: 2.048 ± 0.045	3.64GlyAsn: 3.64 ± 0.073	1.717GlyPro: 1.717 ± 0.04	1.8GlyGln: 1.8 ± 0.05	3.129GlyArg: 3.129 ± 0.065	4.941GlySer: 4.941 ± 0.102	4.05GlyThr: 4.05 ± 0.078	5.382GlyVal: 5.382 ± 0.076	1.006GlyTrp: 1.006 ± 0.041	2.809GlyTyr: 2.809 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.227HisAla: 1.227 ± 0.037	0.283HisCys: 0.283 ± 0.015	0.916HisAsp: 0.916 ± 0.032	0.98HisGlu: 0.98 ± 0.033	1.024HisPhe: 1.024 ± 0.028	1.175HisGly: 1.175 ± 0.036	0.472HisHis: 0.472 ± 0.024	1.03HisIle: 1.03 ± 0.032	1.005HisLys: 1.005 ± 0.03	1.674HisLeu: 1.674 ± 0.045	0.38HisMet: 0.38 ± 0.018	0.691HisAsn: 0.691 ± 0.027	0.996HisPro: 0.996 ± 0.036	0.62HisGln: 0.62 ± 0.024	0.817HisArg: 0.817 ± 0.027	1.061HisSer: 1.061 ± 0.028	0.782HisThr: 0.782 ± 0.025	1.129HisVal: 1.129 ± 0.033	0.279HisTrp: 0.279 ± 0.016	0.723HisTyr: 0.723 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.381IleAla: 5.381 ± 0.084	0.89IleCys: 0.89 ± 0.032	3.518IleAsp: 3.518 ± 0.07	3.526IleGlu: 3.526 ± 0.061	2.586IlePhe: 2.586 ± 0.055	4.043IleGly: 4.043 ± 0.072	1.176IleHis: 1.176 ± 0.035	3.123IleIle: 3.123 ± 0.061	3.484IleLys: 3.484 ± 0.064	5.415IleLeu: 5.415 ± 0.091	1.278IleMet: 1.278 ± 0.044	2.433IleAsn: 2.433 ± 0.048	2.988IlePro: 2.988 ± 0.054	2.143IleGln: 2.143 ± 0.044	2.916IleArg: 2.916 ± 0.053	4.225IleSer: 4.225 ± 0.075	3.127IleThr: 3.127 ± 0.058	4.127IleVal: 4.127 ± 0.066	0.623IleTrp: 0.623 ± 0.029	1.97IleTyr: 1.97 ± 0.047	0.0IleXaa: 0.0 ± 0.0
Lys	5.621LysAla: 5.621 ± 0.088	0.657LysCys: 0.657 ± 0.028	4.359LysAsp: 4.359 ± 0.077	4.408LysGlu: 4.408 ± 0.073	2.832LysPhe: 2.832 ± 0.057	4.126LysGly: 4.126 ± 0.064	1.011LysHis: 1.011 ± 0.032	4.164LysIle: 4.164 ± 0.07	5.392LysLys: 5.392 ± 0.087	5.264LysLeu: 5.264 ± 0.074	2.199LysMet: 2.199 ± 0.041	3.756LysAsn: 3.756 ± 0.06	2.284LysPro: 2.284 ± 0.048	2.072LysGln: 2.072 ± 0.055	2.792LysArg: 2.792 ± 0.057	4.119LysSer: 4.119 ± 0.066	3.63LysThr: 3.63 ± 0.062	4.696LysVal: 4.696 ± 0.074	0.685LysTrp: 0.685 ± 0.029	2.351LysTyr: 2.351 ± 0.051	0.0LysXaa: 0.0 ± 0.0
Leu	7.417LeuAla: 7.417 ± 0.095	1.274LeuCys: 1.274 ± 0.042	5.526LeuAsp: 5.526 ± 0.082	5.5LeuGlu: 5.5 ± 0.084	4.039LeuPhe: 4.039 ± 0.083	6.102LeuGly: 6.102 ± 0.087	1.52LeuHis: 1.52 ± 0.045	4.443LeuIle: 4.443 ± 0.077	6.108LeuLys: 6.108 ± 0.091	7.729LeuLeu: 7.729 ± 0.126	2.411LeuMet: 2.411 ± 0.046	4.088LeuAsn: 4.088 ± 0.067	3.935LeuPro: 3.935 ± 0.066	2.977LeuGln: 2.977 ± 0.063	4.111LeuArg: 4.111 ± 0.07	5.936LeuSer: 5.936 ± 0.082	4.774LeuThr: 4.774 ± 0.071	5.781LeuVal: 5.781 ± 0.078	1.05LeuTrp: 1.05 ± 0.036	2.875LeuTyr: 2.875 ± 0.062	0.0LeuXaa: 0.0 ± 0.0
Met	2.426MetAla: 2.426 ± 0.05	0.23MetCys: 0.23 ± 0.012	1.916MetAsp: 1.916 ± 0.038	1.795MetGlu: 1.795 ± 0.04	1.091MetPhe: 1.091 ± 0.036	2.074MetGly: 2.074 ± 0.043	0.414MetHis: 0.414 ± 0.02	1.452MetIle: 1.452 ± 0.041	2.017MetLys: 2.017 ± 0.044	2.25MetLeu: 2.25 ± 0.048	0.747MetMet: 0.747 ± 0.028	1.577MetAsn: 1.577 ± 0.038	1.084MetPro: 1.084 ± 0.03	0.854MetGln: 0.854 ± 0.029	1.157MetArg: 1.157 ± 0.034	1.738MetSer: 1.738 ± 0.04	1.461MetThr: 1.461 ± 0.033	1.841MetVal: 1.841 ± 0.043	0.23MetTrp: 0.23 ± 0.014	0.703MetTyr: 0.703 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.887AsnAla: 3.887 ± 0.058	0.593AsnCys: 0.593 ± 0.029	2.627AsnAsp: 2.627 ± 0.062	2.648AsnGlu: 2.648 ± 0.045	2.28AsnPhe: 2.28 ± 0.047	4.006AsnGly: 4.006 ± 0.092	0.861AsnHis: 0.861 ± 0.029	2.83AsnIle: 2.83 ± 0.058	2.773AsnLys: 2.773 ± 0.057	4.232AsnLeu: 4.232 ± 0.063	1.276AsnMet: 1.276 ± 0.038	2.007AsnAsn: 2.007 ± 0.059	2.375AsnPro: 2.375 ± 0.053	1.321AsnGln: 1.321 ± 0.034	2.136AsnArg: 2.136 ± 0.05	2.903AsnSer: 2.903 ± 0.056	2.248AsnThr: 2.248 ± 0.054	3.374AsnVal: 3.374 ± 0.064	0.656AsnTrp: 0.656 ± 0.026	1.798AsnTyr: 1.798 ± 0.04	0.0AsnXaa: 0.0 ± 0.0
Pro	3.747ProAla: 3.747 ± 0.097	0.448ProCys: 0.448 ± 0.02	2.484ProAsp: 2.484 ± 0.05	3.317ProGlu: 3.317 ± 0.059	1.816ProPhe: 1.816 ± 0.044	2.59ProGly: 2.59 ± 0.056	0.641ProHis: 0.641 ± 0.023	1.998ProIle: 1.998 ± 0.043	2.375ProLys: 2.375 ± 0.049	3.094ProLeu: 3.094 ± 0.059	1.009ProMet: 1.009 ± 0.034	1.715ProAsn: 1.715 ± 0.046	1.071ProPro: 1.071 ± 0.04	1.311ProGln: 1.311 ± 0.038	1.407ProArg: 1.407 ± 0.041	2.417ProSer: 2.417 ± 0.048	2.024ProThr: 2.024 ± 0.044	3.157ProVal: 3.157 ± 0.065	0.527ProTrp: 0.527 ± 0.021	1.356ProTyr: 1.356 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	2.506GlnAla: 2.506 ± 0.055	0.331GlnCys: 0.331 ± 0.016	1.74GlnAsp: 1.74 ± 0.046	2.122GlnGlu: 2.122 ± 0.054	1.463GlnPhe: 1.463 ± 0.035	2.04GlnGly: 2.04 ± 0.04	0.462GlnHis: 0.462 ± 0.022	1.961GlnIle: 1.961 ± 0.044	2.442GlnLys: 2.442 ± 0.055	2.495GlnLeu: 2.495 ± 0.055	1.047GlnMet: 1.047 ± 0.033	1.63GlnAsn: 1.63 ± 0.037	1.005GlnPro: 1.005 ± 0.031	1.111GlnGln: 1.111 ± 0.036	1.235GlnArg: 1.235 ± 0.033	1.805GlnSer: 1.805 ± 0.042	1.511GlnThr: 1.511 ± 0.03	2.215GlnVal: 2.215 ± 0.042	0.432GlnTrp: 0.432 ± 0.02	1.077GlnTyr: 1.077 ± 0.031	0.0GlnXaa: 0.0 ± 0.0
Arg	3.002ArgAla: 3.002 ± 0.056	0.595ArgCys: 0.595 ± 0.025	2.597ArgAsp: 2.597 ± 0.052	3.088ArgGlu: 3.088 ± 0.061	2.25ArgPhe: 2.25 ± 0.054	2.857ArgGly: 2.857 ± 0.058	0.774ArgHis: 0.774 ± 0.028	2.992ArgIle: 2.992 ± 0.054	2.953ArgLys: 2.953 ± 0.055	4.145ArgLeu: 4.145 ± 0.072	1.276ArgMet: 1.276 ± 0.036	2.138ArgAsn: 2.138 ± 0.04	1.585ArgPro: 1.585 ± 0.043	1.4ArgGln: 1.4 ± 0.037	2.164ArgArg: 2.164 ± 0.052	2.508ArgSer: 2.508 ± 0.055	2.149ArgThr: 2.149 ± 0.041	2.988ArgVal: 2.988 ± 0.059	0.538ArgTrp: 0.538 ± 0.024	1.769ArgTyr: 1.769 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	5.641SerAla: 5.641 ± 0.094	0.866SerCys: 0.866 ± 0.032	3.727SerAsp: 3.727 ± 0.067	4.007SerGlu: 4.007 ± 0.068	3.188SerPhe: 3.188 ± 0.066	5.183SerGly: 5.183 ± 0.086	1.052SerHis: 1.052 ± 0.03	4.172SerIle: 4.172 ± 0.07	4.022SerLys: 4.022 ± 0.062	6.201SerLeu: 6.201 ± 0.086	1.671SerMet: 1.671 ± 0.044	2.772SerAsn: 2.772 ± 0.054	2.219SerPro: 2.219 ± 0.044	1.871SerGln: 1.871 ± 0.046	2.872SerArg: 2.872 ± 0.052	6.271SerSer: 6.271 ± 0.257	3.416SerThr: 3.416 ± 0.075	5.103SerVal: 5.103 ± 0.087	0.94SerTrp: 0.94 ± 0.036	2.396SerTyr: 2.396 ± 0.055	0.0SerXaa: 0.0 ± 0.0
Thr	4.597ThrAla: 4.597 ± 0.073	0.665ThrCys: 0.665 ± 0.024	2.981ThrAsp: 2.981 ± 0.067	3.102ThrGlu: 3.102 ± 0.05	2.51ThrPhe: 2.51 ± 0.057	4.062ThrGly: 4.062 ± 0.07	0.97ThrHis: 0.97 ± 0.037	3.42ThrIle: 3.42 ± 0.072	2.839ThrLys: 2.839 ± 0.058	5.361ThrLeu: 5.361 ± 0.077	1.224ThrMet: 1.224 ± 0.035	2.001ThrAsn: 2.001 ± 0.053	2.572ThrPro: 2.572 ± 0.05	1.448ThrGln: 1.448 ± 0.041	1.998ThrArg: 1.998 ± 0.045	3.38ThrSer: 3.38 ± 0.061	2.956ThrThr: 2.956 ± 0.067	4.132ThrVal: 4.132 ± 0.083	0.67ThrTrp: 0.67 ± 0.025	1.988ThrTyr: 1.988 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	6.317ValAla: 6.317 ± 0.079	1.012ValCys: 1.012 ± 0.033	4.467ValAsp: 4.467 ± 0.068	4.498ValGlu: 4.498 ± 0.08	3.222ValPhe: 3.222 ± 0.065	4.697ValGly: 4.697 ± 0.083	1.142ValHis: 1.142 ± 0.034	4.001ValIle: 4.001 ± 0.066	4.606ValLys: 4.606 ± 0.078	6.143ValLeu: 6.143 ± 0.087	1.819ValMet: 1.819 ± 0.041	3.349ValAsn: 3.349 ± 0.06	2.925ValPro: 2.925 ± 0.051	2.179ValGln: 2.179 ± 0.046	3.261ValArg: 3.261 ± 0.059	5.012ValSer: 5.012 ± 0.079	3.869ValThr: 3.869 ± 0.071	5.359ValVal: 5.359 ± 0.096	0.776ValTrp: 0.776 ± 0.028	2.478ValTyr: 2.478 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.79TrpAla: 0.79 ± 0.025	0.156TrpCys: 0.156 ± 0.014	0.753TrpAsp: 0.753 ± 0.032	0.676TrpGlu: 0.676 ± 0.027	0.577TrpPhe: 0.577 ± 0.025	0.826TrpGly: 0.826 ± 0.03	0.282TrpHis: 0.282 ± 0.016	0.846TrpIle: 0.846 ± 0.027	0.836TrpLys: 0.836 ± 0.032	1.027TrpLeu: 1.027 ± 0.034	0.44TrpMet: 0.44 ± 0.021	0.876TrpAsn: 0.876 ± 0.037	0.339TrpPro: 0.339 ± 0.019	0.408TrpGln: 0.408 ± 0.022	0.521TrpArg: 0.521 ± 0.026	0.84TrpSer: 0.84 ± 0.034	0.783TrpThr: 0.783 ± 0.027	0.771TrpVal: 0.771 ± 0.026	0.179TrpTrp: 0.179 ± 0.014	0.476TrpTyr: 0.476 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.688TyrAla: 2.688 ± 0.055	0.453TyrCys: 0.453 ± 0.023	2.307TyrAsp: 2.307 ± 0.051	2.241TyrGlu: 2.241 ± 0.049	1.765TyrPhe: 1.765 ± 0.042	2.824TyrGly: 2.824 ± 0.066	0.709TyrHis: 0.709 ± 0.026	1.91TyrIle: 1.91 ± 0.047	2.284TyrLys: 2.284 ± 0.048	3.035TyrLeu: 3.035 ± 0.061	0.924TyrMet: 0.924 ± 0.033	1.739TyrAsn: 1.739 ± 0.035	1.424TyrPro: 1.424 ± 0.041	1.157TyrGln: 1.157 ± 0.034	1.856TyrArg: 1.856 ± 0.05	2.439TyrSer: 2.439 ± 0.051	2.059TyrThr: 2.059 ± 0.048	2.451TyrVal: 2.451 ± 0.05	0.492TyrTrp: 0.492 ± 0.021	1.632TyrTyr: 1.632 ± 0.04	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2928 proteins (1075629 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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