Coprinellus micaceus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Psathyrellaceae; Coprinellus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23011 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y7SH21|A0A4Y7SH21_9AGAR Uncharacterized protein OS=Coprinellus micaceus OX=71717 GN=FA13DRAFT_1742332 PE=4 SV=1
MM1 pKa = 7.76GDD3 pKa = 3.42VQPVLKK9 pKa = 10.28AAYY12 pKa = 9.03PPMPNDD18 pKa = 3.89NILGPVAHH26 pKa = 7.48RR27 pKa = 11.84SLQPIPNVFEE37 pKa = 4.72LSQATTLSVDD47 pKa = 3.59DD48 pKa = 4.72GSLNGIYY55 pKa = 8.62DD56 pKa = 3.63TTTIGEE62 pKa = 4.22PSPFAPHH69 pKa = 7.49DD70 pKa = 4.39DD71 pKa = 3.8SLPPSGSIMSALVRR85 pKa = 11.84FFNLDD90 pKa = 3.23MPTNNSDD97 pKa = 3.41ATGTSSNGFDD107 pKa = 4.63SDD109 pKa = 3.41IHH111 pKa = 7.22CDD113 pKa = 4.55LIPATFDD120 pKa = 3.74SNSDD124 pKa = 3.27SSFKK128 pKa = 10.35FVPPIPTGSSSTLQQPDD145 pKa = 3.87ADD147 pKa = 3.93TGSNFDD153 pKa = 4.7SISSFDD159 pKa = 4.2FVPEE163 pKa = 4.21GNQYY167 pKa = 9.49EE168 pKa = 4.39MNMDD172 pKa = 3.19ISFPSLSNDD181 pKa = 2.68AYY183 pKa = 10.08TFGDD187 pKa = 3.26QTAYY191 pKa = 10.35SISHH195 pKa = 6.11IPFPDD200 pKa = 3.32FHH202 pKa = 7.95DD203 pKa = 4.33LAWVYY208 pKa = 10.09IASDD212 pKa = 4.09FEE214 pKa = 4.35HH215 pKa = 6.49TLNDD219 pKa = 3.43SSHH222 pKa = 6.86RR223 pKa = 11.84GLLVQAA229 pKa = 5.14

Molecular weight: 24.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y7TQ73|A0A4Y7TQ73_9AGAR S-adenosyl-L-methionine-dependent methyltransferase OS=Coprinellus micaceus OX=71717 GN=FA13DRAFT_1727665 PE=4 SV=1
MM1 pKa = 7.83PFFTSRR7 pKa = 11.84RR8 pKa = 11.84TRR10 pKa = 11.84RR11 pKa = 11.84THH13 pKa = 6.4AVSTVPRR20 pKa = 11.84TSRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84GFTLPTFGRR34 pKa = 11.84KK35 pKa = 8.59NKK37 pKa = 9.37NRR39 pKa = 11.84VAGGHH44 pKa = 6.32AAALSNPHH52 pKa = 5.09TTRR55 pKa = 11.84SGRR58 pKa = 11.84GHH60 pKa = 7.54AKK62 pKa = 10.15RR63 pKa = 11.84EE64 pKa = 3.99LRR66 pKa = 11.84AMGQGNRR73 pKa = 11.84THH75 pKa = 6.39VPLMTKK81 pKa = 9.78IKK83 pKa = 9.49RR84 pKa = 11.84ALGIRR89 pKa = 11.84SSPRR93 pKa = 11.84HH94 pKa = 4.78STHH97 pKa = 5.05TTTMGSRR104 pKa = 11.84SRR106 pKa = 11.84HH107 pKa = 5.43AIVVV111 pKa = 3.6

Molecular weight: 12.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23011

0

23011

8897202

49

5035

386.6

42.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.261 ± 0.013

1.411 ± 0.008

5.373 ± 0.012

5.92 ± 0.018

3.673 ± 0.009

6.825 ± 0.017

2.677 ± 0.008

4.533 ± 0.01

4.469 ± 0.016

9.314 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.972 ± 0.006

3.288 ± 0.008

6.904 ± 0.02

3.676 ± 0.009

6.67 ± 0.015

8.669 ± 0.018

6.016 ± 0.01

6.243 ± 0.01

1.53 ± 0.006

2.577 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski