Lutimaribacter litoralis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4022 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A521B5X1|A0A521B5X1_9RHOB Glyoxylase beta-lactamase superfamily II OS=Lutimaribacter litoralis OX=1010611 GN=SAMN06265173_102155 PE=4 SV=1
MM1 pKa = 6.76 PTGYY5 pKa = 10.73 LVTLGNYY12 pKa = 9.53 SLNSGDD18 pKa = 4.2 TIGTSTYY25 pKa = 9.68 SFSSASVIGSGSMSYY40 pKa = 10.68 YY41 pKa = 10.07 GRR43 pKa = 11.84 QYY45 pKa = 9.94 WYY47 pKa = 9.89 QSSSYY52 pKa = 9.11 RR53 pKa = 11.84 TITGTYY59 pKa = 9.67 YY60 pKa = 10.57 EE61 pKa = 4.22 ATNGNVYY68 pKa = 9.65 FVPSSTLNLVQSASASTVPSYY89 pKa = 7.7 TTLDD93 pKa = 3.59 DD94 pKa = 3.92 TVEE97 pKa = 4.17 GTGADD102 pKa = 3.89 DD103 pKa = 6.82 LIDD106 pKa = 3.78 ASYY109 pKa = 11.36 GGDD112 pKa = 3.41 PDD114 pKa = 4.72 GDD116 pKa = 3.99 HH117 pKa = 7.03 VDD119 pKa = 4.34 DD120 pKa = 6.46 GIGTGTGGMGDD131 pKa = 3.9 SIEE134 pKa = 4.21 AAGGDD139 pKa = 3.66 DD140 pKa = 3.25 TVYY143 pKa = 10.87 AGSGDD148 pKa = 3.72 DD149 pKa = 4.1 TITGGGGSDD158 pKa = 4.19 YY159 pKa = 11.02 IDD161 pKa = 4.16 GGDD164 pKa = 4.01 GNDD167 pKa = 3.61 VIYY170 pKa = 10.99 GDD172 pKa = 5.1 DD173 pKa = 3.61 VTSAASTAEE182 pKa = 3.93 SMNWSSAGSDD192 pKa = 3.45 EE193 pKa = 4.14 QDD195 pKa = 2.67 ISGGFTQTTGVMDD208 pKa = 3.83 VNVSFSTDD216 pKa = 3.04 GNPNTTFTVEE226 pKa = 4.17 STTTSYY232 pKa = 11.15 VGSGEE237 pKa = 4.31 GFNSTSALEE246 pKa = 4.18 IGSQNTGDD254 pKa = 4.07 AGTVTLDD261 pKa = 3.4 FTTNQPDD268 pKa = 3.69 SFSDD272 pKa = 3.31 NVEE275 pKa = 3.97 NVSFRR280 pKa = 11.84 LNDD283 pKa = 3.1 VDD285 pKa = 4.5 GLQDD289 pKa = 3.25 GWQDD293 pKa = 3.57 VITITAVDD301 pKa = 3.62 ANGNTVTVLLGDD313 pKa = 3.62 NGEE316 pKa = 4.59 DD317 pKa = 3.37 VVSGNTVTADD327 pKa = 3.64 LTRR330 pKa = 11.84 DD331 pKa = 3.38 SYY333 pKa = 11.86 TDD335 pKa = 3.37 ADD337 pKa = 3.83 GSVLVTIPGPVQSISIDD354 pKa = 3.62 YY355 pKa = 11.23 DD356 pKa = 3.25 NGFANGQILIVSDD369 pKa = 3.32 VHH371 pKa = 8.09 FEE373 pKa = 4.4 TIPLTPGDD381 pKa = 3.91 DD382 pKa = 4.11 TILGGAGDD390 pKa = 4.1 DD391 pKa = 4.82 LIYY394 pKa = 11.3 GEE396 pKa = 6.22 DD397 pKa = 3.77 GDD399 pKa = 4.73 DD400 pKa = 3.38 WLGGGDD406 pKa = 4.85 GSDD409 pKa = 3.35 TLIGGVGNDD418 pKa = 3.79 TLAGGAGGDD427 pKa = 4.14 SLSSGAGMDD436 pKa = 3.57 YY437 pKa = 11.07 LDD439 pKa = 4.45 YY440 pKa = 10.64 SGSSAGVDD448 pKa = 3.42 VNLGLGTASGGDD460 pKa = 3.56 AEE462 pKa = 5.24 GDD464 pKa = 3.78 TLAGGLDD471 pKa = 4.32 GIIGSDD477 pKa = 3.24 WDD479 pKa = 4.03 DD480 pKa = 3.49 TLTGYY485 pKa = 11.13 DD486 pKa = 3.49 MQGIDD491 pKa = 5.86 NGTAWTNVFYY501 pKa = 11.03 GGGGNDD507 pKa = 4.01 YY508 pKa = 10.8 IDD510 pKa = 4.07 GAGADD515 pKa = 3.5 DD516 pKa = 4.14 FLYY519 pKa = 11.07 GEE521 pKa = 4.97 EE522 pKa = 4.72 GDD524 pKa = 3.96 DD525 pKa = 4.08 TILGGTGNDD534 pKa = 3.9 LVDD537 pKa = 3.81 GGIGNDD543 pKa = 3.58 SLLGGDD549 pKa = 4.99 GNDD552 pKa = 3.6 TLIGGDD558 pKa = 3.84 GDD560 pKa = 5.6 DD561 pKa = 4.07 ILSGGGGADD570 pKa = 3.05 ILSGGTGNDD579 pKa = 3.33 QITVGSGDD587 pKa = 3.63 TATGGAGDD595 pKa = 4.39 DD596 pKa = 3.73 TFTIQGGGGTITIDD610 pKa = 3.18 GGEE613 pKa = 4.22 GGEE616 pKa = 4.39 GSGDD620 pKa = 3.58 TIDD623 pKa = 5.3 FGGQIDD629 pKa = 4.12 WGSITYY635 pKa = 9.3 TNTVPSDD642 pKa = 3.42 LAGSVTLADD651 pKa = 3.67 GTVVTFSNIEE661 pKa = 4.06 SVVICFKK668 pKa = 10.88 AGTRR672 pKa = 11.84 IEE674 pKa = 4.5 TPHH677 pKa = 6.03 GPRR680 pKa = 11.84 RR681 pKa = 11.84 IEE683 pKa = 4.03 DD684 pKa = 4.09 LRR686 pKa = 11.84 PGDD689 pKa = 3.85 PVITRR694 pKa = 11.84 DD695 pKa = 3.55 NGVQPVRR702 pKa = 11.84 WAGRR706 pKa = 11.84 RR707 pKa = 11.84 RR708 pKa = 11.84 VPGQGEE714 pKa = 3.89 FAPIRR719 pKa = 11.84 FAPGTVGNHH728 pKa = 5.64 RR729 pKa = 11.84 ALIVSPQHH737 pKa = 6.87 RR738 pKa = 11.84 ILHH741 pKa = 6.09 HH742 pKa = 6.49 SPAATLLFNSTEE754 pKa = 4.01 VLVAARR760 pKa = 11.84 HH761 pKa = 5.02 LVNGKK766 pKa = 7.3 TIRR769 pKa = 11.84 QVEE772 pKa = 4.37 KK773 pKa = 10.69 DD774 pKa = 3.39 QIDD777 pKa = 3.87 YY778 pKa = 11.09 VHH780 pKa = 7.08 ILFDD784 pKa = 3.14 RR785 pKa = 11.84 HH786 pKa = 5.94 EE787 pKa = 4.73 IIFAEE792 pKa = 5.17 GAASEE797 pKa = 4.5 SFHH800 pKa = 6.95 PGRR803 pKa = 11.84 HH804 pKa = 5.04 GLNGVMGSARR814 pKa = 11.84 EE815 pKa = 3.87 EE816 pKa = 4.02 LFALFPEE823 pKa = 4.82 LRR825 pKa = 11.84 SDD827 pKa = 3.65 PDD829 pKa = 3.68 SFGDD833 pKa = 3.52 TARR836 pKa = 11.84 PCLRR840 pKa = 11.84 EE841 pKa = 3.8 YY842 pKa = 10.36 EE843 pKa = 4.26 ARR845 pKa = 11.84 LLTPAA850 pKa = 4.61
Molecular weight: 86.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.699
IPC2_protein 3.554
IPC_protein 3.617
Toseland 3.376
ProMoST 3.795
Dawson 3.63
Bjellqvist 3.783
Wikipedia 3.592
Rodwell 3.439
Grimsley 3.274
Solomon 3.617
Lehninger 3.579
Nozaki 3.732
DTASelect 4.05
Thurlkill 3.427
EMBOSS 3.592
Sillero 3.745
Patrickios 1.278
IPC_peptide 3.617
IPC2_peptide 3.719
IPC2.peptide.svr19 3.693
Protein with the highest isoelectric point:
>tr|A0A521E4U2|A0A521E4U2_9RHOB Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase OS=Lutimaribacter litoralis OX=1010611 GN=SAMN06265173_11478 PE=4 SV=1
MM1 pKa = 7.61 NANQLINMVLRR12 pKa = 11.84 MVLRR16 pKa = 11.84 KK17 pKa = 9.48 AVNTGVNRR25 pKa = 11.84 LAGGKK30 pKa = 9.44 SGPQGVKK37 pKa = 8.45 AAKK40 pKa = 9.83 RR41 pKa = 11.84 ARR43 pKa = 11.84 QAMRR47 pKa = 11.84 VTRR50 pKa = 11.84 RR51 pKa = 11.84 LNKK54 pKa = 9.82 FF55 pKa = 3.06
Molecular weight: 6.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.574
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.31
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4022
0
4022
1232936
25
2127
306.5
33.33
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.873 ± 0.046
0.895 ± 0.014
6.183 ± 0.037
5.509 ± 0.032
3.703 ± 0.022
8.665 ± 0.04
2.089 ± 0.018
5.286 ± 0.026
3.495 ± 0.033
10.042 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.924 ± 0.02
2.783 ± 0.022
5.086 ± 0.025
3.53 ± 0.02
6.392 ± 0.038
5.038 ± 0.026
5.554 ± 0.028
7.25 ± 0.03
1.378 ± 0.016
2.325 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here