Sneathiella sp. DP05

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sneathiellales; Sneathiellaceae; Sneathiella

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L8W3M3|A0A6L8W3M3_9PROT ATP-binding cassette domain-containing protein OS=Sneathiella sp. DP05 OX=2606216 GN=GQE98_00695 PE=4 SV=1
MM1 pKa = 7.26LHH3 pKa = 6.0VPLRR7 pKa = 11.84PTTLTLVFTTALPFMLFSLPVSAEE31 pKa = 3.8EE32 pKa = 3.91EE33 pKa = 4.45SFPKK37 pKa = 9.34ITGEE41 pKa = 3.98LSVEE45 pKa = 4.27VEE47 pKa = 4.29NDD49 pKa = 2.62WTYY52 pKa = 11.66QSDD55 pKa = 4.56DD56 pKa = 3.84PDD58 pKa = 5.45AEE60 pKa = 4.44INDD63 pKa = 4.59LYY65 pKa = 9.65PTVVLGTNVAFTEE78 pKa = 4.39VFSLNFEE85 pKa = 4.21ATLEE89 pKa = 4.07PVEE92 pKa = 5.17DD93 pKa = 3.87ATSDD97 pKa = 3.71RR98 pKa = 11.84AFEE101 pKa = 4.51DD102 pKa = 2.93LGGYY106 pKa = 10.26LNIITVNYY114 pKa = 10.18DD115 pKa = 3.18GEE117 pKa = 4.45AVSAYY122 pKa = 10.17AGKK125 pKa = 8.49FTPNFGIAWDD135 pKa = 3.66IAPGIFGTNLNEE147 pKa = 4.36DD148 pKa = 3.78YY149 pKa = 10.8EE150 pKa = 4.32LAEE153 pKa = 4.37MIGFGGGFHH162 pKa = 6.96FEE164 pKa = 3.94AAGEE168 pKa = 4.2HH169 pKa = 6.29TISASTFFQDD179 pKa = 4.27TSFLSNSVGTQRR191 pKa = 11.84GPLRR195 pKa = 11.84LSDD198 pKa = 3.48GGAGNTEE205 pKa = 4.14NFSSFAVALDD215 pKa = 3.71GNFQKK220 pKa = 11.09LPGFRR225 pKa = 11.84YY226 pKa = 9.91HH227 pKa = 7.4LGLSSLGAGEE237 pKa = 5.16DD238 pKa = 3.87GNDD241 pKa = 3.37RR242 pKa = 11.84QLGYY246 pKa = 10.91AIGGEE251 pKa = 4.15YY252 pKa = 10.48AFNIGEE258 pKa = 4.73DD259 pKa = 3.74VTLSPMAEE267 pKa = 3.54YY268 pKa = 10.8VYY270 pKa = 10.46FDD272 pKa = 3.61NYY274 pKa = 11.14GGIDD278 pKa = 4.03NDD280 pKa = 3.88TAKK283 pKa = 10.93YY284 pKa = 7.38FTAGLALNYY293 pKa = 8.93EE294 pKa = 4.09NWAASSTYY302 pKa = 7.81QLRR305 pKa = 11.84DD306 pKa = 3.53TEE308 pKa = 4.51VGGVMTDD315 pKa = 3.25DD316 pKa = 3.84HH317 pKa = 8.24VVDD320 pKa = 4.12LTVGYY325 pKa = 10.21VFDD328 pKa = 4.28MGLGVAAAWRR338 pKa = 11.84SAEE341 pKa = 3.93EE342 pKa = 4.35DD343 pKa = 3.81NIDD346 pKa = 4.41SEE348 pKa = 4.97GLGLLLSYY356 pKa = 10.8AIDD359 pKa = 3.82FF360 pKa = 4.48

Molecular weight: 38.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L8W2N0|A0A6L8W2N0_9PROT Acyl-CoA dehydrogenase OS=Sneathiella sp. DP05 OX=2606216 GN=GQE98_01630 PE=3 SV=1
MM1 pKa = 6.96TARR4 pKa = 11.84SARR7 pKa = 11.84NGRR10 pKa = 11.84NQGGKK15 pKa = 8.11SRR17 pKa = 11.84KK18 pKa = 9.35KK19 pKa = 10.16GGGGSGSGLTKK30 pKa = 10.43SSRR33 pKa = 11.84SNQQRR38 pKa = 11.84AARR41 pKa = 11.84KK42 pKa = 8.6MNLKK46 pKa = 10.04AHH48 pKa = 6.12GRR50 pKa = 11.84HH51 pKa = 5.55AARR54 pKa = 11.84RR55 pKa = 11.84QLKK58 pKa = 9.75KK59 pKa = 9.82IQAATRR65 pKa = 11.84IAAA68 pKa = 3.79

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3523

0

3523

1128886

29

2065

320.4

35.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.006 ± 0.043

0.898 ± 0.011

5.742 ± 0.033

6.514 ± 0.039

4.093 ± 0.028

8.025 ± 0.036

1.965 ± 0.018

6.543 ± 0.031

4.729 ± 0.033

10.032 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.727 ± 0.017

3.468 ± 0.021

4.532 ± 0.022

3.067 ± 0.021

5.537 ± 0.032

5.946 ± 0.027

5.339 ± 0.024

7.018 ± 0.032

1.202 ± 0.015

2.62 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski