Cytobacillus depressus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Cytobacillus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4971 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6L3VBS5|A0A6L3VBS5_9BACI Heme chaperone HemW OS=Cytobacillus depressus OX=1602942 GN=F7731_03405 PE=3 SV=1
MM1 pKa = 7.71EE2 pKa = 5.83IISYY6 pKa = 7.93TVEE9 pKa = 3.84QLQDD13 pKa = 3.27PTGILLGEE21 pKa = 4.12RR22 pKa = 11.84YY23 pKa = 9.98EE24 pKa = 4.22FFLDD28 pKa = 3.93ILVDD32 pKa = 5.18DD33 pKa = 4.53EE34 pKa = 5.41DD35 pKa = 3.92EE36 pKa = 5.01LYY38 pKa = 10.86SEE40 pKa = 5.42SGLQLRR46 pKa = 11.84ILFYY50 pKa = 10.79KK51 pKa = 10.7HH52 pKa = 6.27EE53 pKa = 4.83DD54 pKa = 3.31DD55 pKa = 4.89YY56 pKa = 11.84RR57 pKa = 11.84ILNYY61 pKa = 10.35HH62 pKa = 6.83FLTSEE67 pKa = 4.32TILDD71 pKa = 3.75FALDD75 pKa = 3.55EE76 pKa = 4.7DD77 pKa = 4.25EE78 pKa = 5.01EE79 pKa = 5.1KK80 pKa = 10.79FVAQFCRR87 pKa = 11.84EE88 pKa = 4.22HH89 pKa = 7.51YY90 pKa = 10.87VEE92 pKa = 4.68ASEE95 pKa = 5.21NEE97 pKa = 3.96

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6L3V5Y9|A0A6L3V5Y9_9BACI Alpha/beta hydrolase OS=Cytobacillus depressus OX=1602942 GN=F7731_09485 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.42KK9 pKa = 8.05RR10 pKa = 11.84KK11 pKa = 8.77RR12 pKa = 11.84SKK14 pKa = 9.05VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTPNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4971

0

4971

1449228

23

1786

291.5

32.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.049 ± 0.04

0.718 ± 0.011

4.851 ± 0.029

7.378 ± 0.05

4.702 ± 0.028

7.054 ± 0.033

2.044 ± 0.016

8.363 ± 0.037

7.11 ± 0.033

9.74 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.016

4.598 ± 0.024

3.666 ± 0.02

3.569 ± 0.024

3.887 ± 0.023

6.027 ± 0.027

5.247 ± 0.023

6.804 ± 0.03

1.009 ± 0.013

3.415 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski