Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc)

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Akkermansiaceae; Akkermansia; Akkermansia muciniphila

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2137 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2UNM6|B2UNM6_AKKM8 ABC transporter related OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_1929 PE=4 SV=1
MM1 pKa = 7.79ADD3 pKa = 3.58IDD5 pKa = 4.42EE6 pKa = 4.59KK7 pKa = 10.88TPLNVPGKK15 pKa = 9.92FYY17 pKa = 11.24VDD19 pKa = 4.23SSCIDD24 pKa = 3.44CDD26 pKa = 3.82LCRR29 pKa = 11.84EE30 pKa = 4.13TAPEE34 pKa = 3.86NFGRR38 pKa = 11.84DD39 pKa = 3.38DD40 pKa = 4.45DD41 pKa = 4.8EE42 pKa = 4.59GVSYY46 pKa = 10.87VKK48 pKa = 10.43KK49 pKa = 10.72QPDD52 pKa = 3.36NDD54 pKa = 4.08EE55 pKa = 4.3EE56 pKa = 4.53LSACEE61 pKa = 4.36EE62 pKa = 4.08ALEE65 pKa = 4.47GCPVEE70 pKa = 5.71AIGDD74 pKa = 3.84DD75 pKa = 4.24GEE77 pKa = 4.18

Molecular weight: 8.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2UR75|B2UR75_AKKM8 Peptidase M15B and M15C DD-carboxypeptidase VanY/endolysin OS=Akkermansia muciniphila (strain ATCC BAA-835 / DSM 22959 / JCM 33894 / BCRC 81048 / CCUG 64013 / CIP 107961 / Muc) OX=349741 GN=Amuc_1134 PE=4 SV=1
MM1 pKa = 7.58GSLKK5 pKa = 10.12KK6 pKa = 10.09RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 9.95AKK11 pKa = 9.63INKK14 pKa = 8.37HH15 pKa = 5.03KK16 pKa = 10.08RR17 pKa = 11.84SKK19 pKa = 10.68RR20 pKa = 11.84MRR22 pKa = 11.84QNRR25 pKa = 11.84HH26 pKa = 4.76KK27 pKa = 10.76KK28 pKa = 8.45RR29 pKa = 11.84LRR31 pKa = 11.84YY32 pKa = 9.57KK33 pKa = 10.44SS34 pKa = 3.35

Molecular weight: 4.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2137

0

2137

771359

30

3152

361.0

39.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.435 ± 0.058

1.408 ± 0.024

5.107 ± 0.036

6.382 ± 0.051

3.975 ± 0.034

7.985 ± 0.077

2.075 ± 0.021

5.349 ± 0.044

4.762 ± 0.051

9.908 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.025

3.637 ± 0.043

5.097 ± 0.054

3.206 ± 0.03

6.244 ± 0.051

6.293 ± 0.041

5.207 ± 0.069

6.676 ± 0.046

1.449 ± 0.023

2.961 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski