Idiomarina sp. A28L

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Idiomarinaceae; Idiomarina; unclassified Idiomarina

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2298 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7RZ01|F7RZ01_9GAMM P-type ATPase translocating P-type ATPase translocating OS=Idiomarina sp. A28L OX=1036674 GN=A28LD_1487 PE=4 SV=1
MM1 pKa = 7.11MKK3 pKa = 9.97INSFLLPVVSAGMLVFAAQADD24 pKa = 4.08TVYY27 pKa = 10.53TSDD30 pKa = 4.15SSVTAYY36 pKa = 10.78DD37 pKa = 4.21PIFPSTAYY45 pKa = 10.7SDD47 pKa = 3.76WVNQACTINPDD58 pKa = 3.11VGLDD62 pKa = 3.67ADD64 pKa = 4.37WEE66 pKa = 4.51NPHH69 pKa = 7.13PSYY72 pKa = 11.22QFGTNVHH79 pKa = 6.69PWQSGSFLNAQWINAWSDD97 pKa = 2.84INARR101 pKa = 11.84GPLGQSWTKK110 pKa = 10.48YY111 pKa = 6.76STEE114 pKa = 3.55ISGQGEE120 pKa = 4.14FVLDD124 pKa = 4.55LLADD128 pKa = 3.8NCSWIYY134 pKa = 10.59IDD136 pKa = 3.53GTLVGFQAVTSLAQKK151 pKa = 10.9YY152 pKa = 8.59PVTLNGDD159 pKa = 3.39HH160 pKa = 6.67TLDD163 pKa = 3.83FLIFDD168 pKa = 4.57GGGLAGGMFRR178 pKa = 11.84LEE180 pKa = 4.47TNTGTVFVDD189 pKa = 3.44SDD191 pKa = 3.82NDD193 pKa = 3.76GLTDD197 pKa = 4.52AEE199 pKa = 4.18EE200 pKa = 4.21ALYY203 pKa = 8.52GTDD206 pKa = 4.11PLNPDD211 pKa = 3.14SDD213 pKa = 4.16GDD215 pKa = 3.96GFTDD219 pKa = 4.11GEE221 pKa = 4.26EE222 pKa = 4.3VAAGTDD228 pKa = 3.64PNDD231 pKa = 4.09PNDD234 pKa = 3.84FPVFDD239 pKa = 4.25TDD241 pKa = 5.55GDD243 pKa = 4.62GVWDD247 pKa = 5.0IDD249 pKa = 4.05DD250 pKa = 4.39ACPNTSEE257 pKa = 4.69GVLVDD262 pKa = 3.41QFGCSGVQNIALACSCEE279 pKa = 4.05GSDD282 pKa = 6.12DD283 pKa = 3.67NSPWKK288 pKa = 10.72NHH290 pKa = 4.64GQYY293 pKa = 10.38VSCVAKK299 pKa = 10.74AKK301 pKa = 10.74NNEE304 pKa = 4.07VNNGLITEE312 pKa = 4.52AEE314 pKa = 4.22GSEE317 pKa = 4.28LVSAAAQSSCGKK329 pKa = 9.81RR330 pKa = 11.84DD331 pKa = 3.41NGNKK335 pKa = 9.78GKK337 pKa = 10.41NKK339 pKa = 10.28

Molecular weight: 36.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7RWD3|F7RWD3_9GAMM tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG OS=Idiomarina sp. A28L OX=1036674 GN=mnmG PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84KK13 pKa = 7.97RR14 pKa = 11.84THH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.31QLAAA44 pKa = 4.14

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2298

0

2298

783584

29

2854

341.0

37.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.787 ± 0.05

0.834 ± 0.015

5.243 ± 0.04

6.72 ± 0.05

4.079 ± 0.03

7.052 ± 0.044

2.227 ± 0.029

6.03 ± 0.038

4.123 ± 0.049

10.411 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.485 ± 0.025

4.122 ± 0.029

4.157 ± 0.031

4.608 ± 0.039

5.674 ± 0.042

6.154 ± 0.041

5.114 ± 0.039

7.053 ± 0.04

1.291 ± 0.02

2.834 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski