Beilong virus
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q287Y1|Q287Y1_9MONO Nucleocapsid OS=Beilong virus OX=341053 GN=N PE=3 SV=1
MM1 pKa = 7.73 SDD3 pKa = 3.66 YY4 pKa = 11.3 NPDD7 pKa = 3.21 VLQQLVKK14 pKa = 10.94 DD15 pKa = 4.5 GIKK18 pKa = 9.87 TIEE21 pKa = 4.64 LLQQSPEE28 pKa = 4.27 DD29 pKa = 3.66 FQKK32 pKa = 10.02 TYY34 pKa = 10.56 GRR36 pKa = 11.84 SAIQEE41 pKa = 3.9 PSTRR45 pKa = 11.84 ARR47 pKa = 11.84 IQSWEE52 pKa = 4.05 SRR54 pKa = 11.84 NPDD57 pKa = 2.98 HH58 pKa = 8.22 DD59 pKa = 3.87 YY60 pKa = 11.2 TGNKK64 pKa = 7.4 NQRR67 pKa = 11.84 SEE69 pKa = 4.25 GAKK72 pKa = 9.54 EE73 pKa = 3.59 RR74 pKa = 11.84 ANKK77 pKa = 10.16 SEE79 pKa = 4.19 SAGTASADD87 pKa = 3.54 GGHH90 pKa = 6.72 GDD92 pKa = 3.68 KK93 pKa = 10.73 PSNNGGDD100 pKa = 3.65 SQNEE104 pKa = 4.14 YY105 pKa = 10.4 QGSDD109 pKa = 3.36 QQVWDD114 pKa = 3.49 AAYY117 pKa = 11.07 NDD119 pKa = 4.47 GNSGGAWGGPTGGLPTAGEE138 pKa = 3.97 RR139 pKa = 11.84 GYY141 pKa = 9.98 PITTGNQEE149 pKa = 4.06 FEE151 pKa = 4.82 GYY153 pKa = 10.34 HH154 pKa = 6.45 PDD156 pKa = 3.57 GPVDD160 pKa = 3.27 ARR162 pKa = 11.84 EE163 pKa = 3.96 YY164 pKa = 10.21 NQISSMDD171 pKa = 3.72 HH172 pKa = 5.74 EE173 pKa = 4.57 MSAAEE178 pKa = 4.06 TLGSSTQLTTMRR190 pKa = 11.84 NATTDD195 pKa = 3.38 DD196 pKa = 3.37 FAKK199 pKa = 10.19 IFEE202 pKa = 4.48 EE203 pKa = 4.86 GTPKK207 pKa = 9.59 VHH209 pKa = 6.94 RR210 pKa = 11.84 RR211 pKa = 11.84 LRR213 pKa = 11.84 GITAVVPAQKK223 pKa = 10.33 QPGAVGGPVKK233 pKa = 10.57 KK234 pKa = 9.83 GTDD237 pKa = 3.22 GSTASTLLGDD247 pKa = 3.97 VPLSGSGAIPNVHH260 pKa = 7.08 PSLLHH265 pKa = 4.98 QPKK268 pKa = 10.34 KK269 pKa = 10.18 NAHH272 pKa = 6.42 AEE274 pKa = 4.0 NAQGSVQDD282 pKa = 3.7 VSTTGAIGQSDD293 pKa = 3.88 EE294 pKa = 4.43 AAHH297 pKa = 6.78 FDD299 pKa = 4.02 QEE301 pKa = 4.88 IEE303 pKa = 4.23 GKK305 pKa = 10.62 LNLVLKK311 pKa = 10.3 EE312 pKa = 4.38 LDD314 pKa = 4.63 LISKK318 pKa = 10.15 KK319 pKa = 10.38 LDD321 pKa = 3.35 YY322 pKa = 10.97 LPEE325 pKa = 5.38 IKK327 pKa = 10.58 EE328 pKa = 4.09 EE329 pKa = 3.65 IRR331 pKa = 11.84 NINKK335 pKa = 10.34 KK336 pKa = 8.04 ITNLSLGLSTVEE348 pKa = 4.21 NYY350 pKa = 9.38 IKK352 pKa = 11.05 SMMIIIPSSGKK363 pKa = 9.59 PDD365 pKa = 3.5 VNDD368 pKa = 3.66 TAEE371 pKa = 4.08 VNPDD375 pKa = 3.29 LKK377 pKa = 11.16 AVIGRR382 pKa = 11.84 DD383 pKa = 3.0 KK384 pKa = 11.08 TRR386 pKa = 11.84 GLKK389 pKa = 9.92 EE390 pKa = 4.15 VTTHH394 pKa = 7.59 RR395 pKa = 11.84 SDD397 pKa = 5.14 LEE399 pKa = 4.16 SLDD402 pKa = 4.21 LLSPATSEE410 pKa = 4.3 IDD412 pKa = 3.66 PKK414 pKa = 11.45 YY415 pKa = 9.84 LTQDD419 pKa = 3.09 LDD421 pKa = 3.99 FTKK424 pKa = 10.93 SNAANFVPGNDD435 pKa = 2.96 ASSFYY440 pKa = 10.54 TIVAMIKK447 pKa = 10.89 GEE449 pKa = 4.07 VTDD452 pKa = 3.54 VKK454 pKa = 10.58 RR455 pKa = 11.84 QQEE458 pKa = 4.26 LIKK461 pKa = 10.26 WVSDD465 pKa = 3.46 SMNKK469 pKa = 9.75 HH470 pKa = 6.08 PMGQIYY476 pKa = 9.68 RR477 pKa = 11.84 VVRR480 pKa = 11.84 EE481 pKa = 4.12 ALDD484 pKa = 4.0 AEE486 pKa = 4.68 SDD488 pKa = 3.86 SSSSSDD494 pKa = 2.92 SDD496 pKa = 3.39
Molecular weight: 53.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.938
IPC2_protein 4.889
IPC_protein 4.851
Toseland 4.749
ProMoST 4.952
Dawson 4.838
Bjellqvist 4.978
Wikipedia 4.711
Rodwell 4.724
Grimsley 4.66
Solomon 4.825
Lehninger 4.787
Nozaki 4.94
DTASelect 5.13
Thurlkill 4.749
EMBOSS 4.749
Sillero 5.003
Patrickios 4.253
IPC_peptide 4.838
IPC2_peptide 5.003
IPC2.peptide.svr19 4.947
Protein with the highest isoelectric point:
>tr|Q287X8|Q287X8_9MONO Isoform of Q287Y0 V protein OS=Beilong virus OX=341053 GN=P/V/C PE=4 SV=1
MM1 pKa = 7.5 SSSSLSRR8 pKa = 11.84 MEE10 pKa = 4.03 SRR12 pKa = 11.84 LSSFYY17 pKa = 11.07 SRR19 pKa = 11.84 VRR21 pKa = 11.84 KK22 pKa = 7.18 TFRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 TAEE30 pKa = 3.76 VPSRR34 pKa = 11.84 SRR36 pKa = 11.84 RR37 pKa = 11.84 QEE39 pKa = 3.83 PEE41 pKa = 4.03 SKK43 pKa = 10.26 VGSPAIPIMITPVIKK58 pKa = 10.11 IRR60 pKa = 11.84 EE61 pKa = 4.03 ARR63 pKa = 11.84 EE64 pKa = 3.76 LKK66 pKa = 10.45 RR67 pKa = 11.84 EE68 pKa = 3.96 QTRR71 pKa = 11.84 ARR73 pKa = 11.84 ARR75 pKa = 11.84 EE76 pKa = 3.95 LLQLMEE82 pKa = 5.12 DD83 pKa = 3.91 MEE85 pKa = 5.28 INPQITGVTPKK96 pKa = 10.06 TSTKK100 pKa = 10.47 GQINRR105 pKa = 11.84 YY106 pKa = 8.12 GMLRR110 pKa = 11.84 IMMGIVAEE118 pKa = 4.31 LGEE121 pKa = 4.49 VPQVDD126 pKa = 4.82 FPRR129 pKa = 11.84 LEE131 pKa = 4.01 KK132 pKa = 10.83 EE133 pKa = 4.67 GILSQQEE140 pKa = 4.06 IKK142 pKa = 10.71 NLRR145 pKa = 11.84 DD146 pKa = 3.7 TIPTVQLMLEE156 pKa = 4.35 SIIRR160 pKa = 3.68
Molecular weight: 18.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.321
IPC2_protein 9.545
IPC_protein 10.438
Toseland 10.847
ProMoST 10.906
Dawson 10.906
Bjellqvist 10.643
Wikipedia 11.14
Rodwell 11.023
Grimsley 10.935
Solomon 11.096
Lehninger 11.067
Nozaki 10.818
DTASelect 10.643
Thurlkill 10.833
EMBOSS 11.272
Sillero 10.847
Patrickios 10.76
IPC_peptide 11.111
IPC2_peptide 9.414
IPC2.peptide.svr19 8.965
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
3
11
5903
76
2172
536.6
60.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.166 ± 0.69
1.745 ± 0.322
5.319 ± 0.448
5.658 ± 0.408
3.371 ± 0.441
6.437 ± 0.85
1.999 ± 0.303
6.844 ± 0.485
5.607 ± 0.368
9.08 ± 0.941
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.965 ± 0.223
5.438 ± 0.348
4.794 ± 0.501
3.761 ± 0.383
5.336 ± 0.505
7.86 ± 0.485
6.37 ± 0.418
6.166 ± 0.385
1.067 ± 0.103
4.015 ± 0.39
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here