Vibrio phage VBP47

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Fuhrmanvirinae; Stoningtonvirus; Vibrio virus VBP47

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4SQQ9|M4SQQ9_9CAUD Uncharacterized protein OS=Vibrio phage VBP47 OX=754073 GN=VPNG_00087 PE=4 SV=1
MM1 pKa = 7.26NVHH4 pKa = 7.53LYY6 pKa = 10.19RR7 pKa = 11.84VIEE10 pKa = 4.32CDD12 pKa = 3.14GSTMYY17 pKa = 10.73ILLSEE22 pKa = 4.83GGWHH26 pKa = 6.97PVTADD31 pKa = 3.1ADD33 pKa = 3.59IDD35 pKa = 3.6EE36 pKa = 5.04EE37 pKa = 4.83YY38 pKa = 11.17AFKK41 pKa = 10.89SLTHH45 pKa = 6.5NGPDD49 pKa = 3.32SDD51 pKa = 4.15YY52 pKa = 11.54GAAFLDD58 pKa = 3.85EE59 pKa = 4.93DD60 pKa = 4.45KK61 pKa = 11.16SVEE64 pKa = 4.08MLLMDD69 pKa = 3.97VAFKK73 pKa = 10.73EE74 pKa = 4.73AVAHH78 pKa = 6.0MKK80 pKa = 9.05MCALLDD86 pKa = 3.64MM87 pKa = 5.75

Molecular weight: 9.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SLB3|M4SLB3_9CAUD Uncharacterized protein OS=Vibrio phage VBP47 OX=754073 GN=VPNG_00113 PE=4 SV=1
MM1 pKa = 8.04KK2 pKa = 9.93IRR4 pKa = 11.84KK5 pKa = 8.27RR6 pKa = 11.84CKK8 pKa = 9.17IRR10 pKa = 11.84SDD12 pKa = 3.3DD13 pKa = 4.59RR14 pKa = 11.84RR15 pKa = 11.84WQLFWTTQEE24 pKa = 3.2EE25 pKa = 5.3RR26 pKa = 11.84YY27 pKa = 9.52RR28 pKa = 11.84VAKK31 pKa = 9.62TRR33 pKa = 11.84VKK35 pKa = 10.45IEE37 pKa = 3.56TLQNYY42 pKa = 8.07RR43 pKa = 11.84KK44 pKa = 9.78RR45 pKa = 11.84RR46 pKa = 11.84DD47 pKa = 3.51RR48 pKa = 11.84KK49 pKa = 8.0VVSYY53 pKa = 10.02FSEE56 pKa = 3.99LHH58 pKa = 4.94LTKK61 pKa = 10.42LGKK64 pKa = 9.46MYY66 pKa = 10.68NFSCMLTGDD75 pKa = 3.8TPLPEE80 pKa = 4.12TFHH83 pKa = 6.79WSFTNISKK91 pKa = 10.08NRR93 pKa = 3.61

Molecular weight: 11.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

114

0

114

24027

50

3220

210.8

23.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.053 ± 0.474

0.974 ± 0.158

6.722 ± 0.221

7.442 ± 0.237

3.808 ± 0.125

6.613 ± 0.277

1.894 ± 0.14

5.719 ± 0.164

6.48 ± 0.283

8.153 ± 0.261

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.997 ± 0.164

5.277 ± 0.25

3.625 ± 0.195

3.996 ± 0.246

4.645 ± 0.188

5.893 ± 0.249

6.164 ± 0.203

6.468 ± 0.177

1.361 ± 0.122

3.717 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski