Amino acid dipepetide frequency for Vibrio phage VBP47

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.075AlaAla: 8.075 ± 1.67
0.499AlaCys: 0.499 ± 0.141
4.495AlaAsp: 4.495 ± 0.409
5.161AlaGlu: 5.161 ± 0.65
3.205AlaPhe: 3.205 ± 0.362
5.119AlaGly: 5.119 ± 0.63
1.332AlaHis: 1.332 ± 0.203
4.745AlaIle: 4.745 ± 0.444
6.326AlaLys: 6.326 ± 0.598
6.618AlaLeu: 6.618 ± 0.562
1.79AlaMet: 1.79 ± 0.276
3.912AlaAsn: 3.912 ± 0.829
2.872AlaPro: 2.872 ± 0.517
3.704AlaGln: 3.704 ± 0.661
3.579AlaArg: 3.579 ± 0.736
4.786AlaSer: 4.786 ± 0.738
5.286AlaThr: 5.286 ± 0.755
5.161AlaVal: 5.161 ± 0.384
1.041AlaTrp: 1.041 ± 0.211
2.83AlaTyr: 2.83 ± 0.34
0.0AlaXaa: 0.0 ± 0.0
Cys
0.957CysAla: 0.957 ± 0.264
0.208CysCys: 0.208 ± 0.079
0.583CysAsp: 0.583 ± 0.145
0.916CysGlu: 0.916 ± 0.23
0.416CysPhe: 0.416 ± 0.129
0.708CysGly: 0.708 ± 0.165
0.208CysHis: 0.208 ± 0.102
0.708CysIle: 0.708 ± 0.187
0.708CysLys: 0.708 ± 0.207
0.791CysLeu: 0.791 ± 0.201
0.458CysMet: 0.458 ± 0.166
0.291CysAsn: 0.291 ± 0.125
0.416CysPro: 0.416 ± 0.152
0.333CysGln: 0.333 ± 0.13
0.208CysArg: 0.208 ± 0.086
0.416CysSer: 0.416 ± 0.127
0.666CysThr: 0.666 ± 0.161
0.416CysVal: 0.416 ± 0.16
0.166CysTrp: 0.166 ± 0.082
0.166CysTyr: 0.166 ± 0.098
0.0CysXaa: 0.0 ± 0.0
Asp
5.411AspAla: 5.411 ± 0.549
0.499AspCys: 0.499 ± 0.15
3.912AspAsp: 3.912 ± 0.516
4.828AspGlu: 4.828 ± 0.614
2.747AspPhe: 2.747 ± 0.395
5.286AspGly: 5.286 ± 0.527
1.457AspHis: 1.457 ± 0.252
4.121AspIle: 4.121 ± 0.481
3.205AspLys: 3.205 ± 0.44
6.077AspLeu: 6.077 ± 0.407
2.081AspMet: 2.081 ± 0.308
3.746AspAsn: 3.746 ± 0.461
2.705AspPro: 2.705 ± 0.328
2.664AspGln: 2.664 ± 0.357
2.705AspArg: 2.705 ± 0.342
3.621AspSer: 3.621 ± 0.361
4.495AspThr: 4.495 ± 0.359
4.245AspVal: 4.245 ± 0.354
1.207AspTrp: 1.207 ± 0.26
2.206AspTyr: 2.206 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
6.243GluAla: 6.243 ± 0.65
0.832GluCys: 0.832 ± 0.248
4.786GluAsp: 4.786 ± 0.537
6.659GluGlu: 6.659 ± 0.749
3.538GluPhe: 3.538 ± 0.327
4.662GluGly: 4.662 ± 0.525
0.874GluHis: 0.874 ± 0.151
4.578GluIle: 4.578 ± 0.478
4.079GluLys: 4.079 ± 0.401
7.034GluLeu: 7.034 ± 0.706
3.038GluMet: 3.038 ± 0.35
3.496GluAsn: 3.496 ± 0.37
1.706GluPro: 1.706 ± 0.254
3.122GluGln: 3.122 ± 0.567
3.122GluArg: 3.122 ± 0.356
4.121GluSer: 4.121 ± 0.512
3.996GluThr: 3.996 ± 0.538
4.495GluVal: 4.495 ± 0.481
0.874GluTrp: 0.874 ± 0.215
3.163GluTyr: 3.163 ± 0.332
0.0GluXaa: 0.0 ± 0.0
Phe
1.956PheAla: 1.956 ± 0.294
0.499PheCys: 0.499 ± 0.189
2.872PheAsp: 2.872 ± 0.325
2.539PheGlu: 2.539 ± 0.416
1.082PhePhe: 1.082 ± 0.184
2.747PheGly: 2.747 ± 0.319
0.666PheHis: 0.666 ± 0.191
2.289PheIle: 2.289 ± 0.285
2.789PheLys: 2.789 ± 0.34
2.955PheLeu: 2.955 ± 0.391
1.374PheMet: 1.374 ± 0.216
2.664PheAsn: 2.664 ± 0.372
1.207PhePro: 1.207 ± 0.223
1.956PheGln: 1.956 ± 0.282
1.998PheArg: 1.998 ± 0.286
2.789PheSer: 2.789 ± 0.313
2.248PheThr: 2.248 ± 0.301
1.956PheVal: 1.956 ± 0.27
0.208PheTrp: 0.208 ± 0.081
1.29PheTyr: 1.29 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
5.078GlyAla: 5.078 ± 0.585
0.541GlyCys: 0.541 ± 0.195
4.578GlyAsp: 4.578 ± 0.442
4.745GlyGlu: 4.745 ± 0.394
2.914GlyPhe: 2.914 ± 0.418
4.62GlyGly: 4.62 ± 0.663
1.207GlyHis: 1.207 ± 0.254
4.204GlyIle: 4.204 ± 0.372
3.954GlyLys: 3.954 ± 0.529
4.578GlyLeu: 4.578 ± 0.537
1.956GlyMet: 1.956 ± 0.261
3.746GlyAsn: 3.746 ± 0.412
1.415GlyPro: 1.415 ± 0.305
2.289GlyGln: 2.289 ± 0.352
2.955GlyArg: 2.955 ± 0.358
3.912GlySer: 3.912 ± 0.453
4.953GlyThr: 4.953 ± 0.547
4.537GlyVal: 4.537 ± 0.565
1.29GlyTrp: 1.29 ± 0.285
2.664GlyTyr: 2.664 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
1.207HisAla: 1.207 ± 0.264
0.333HisCys: 0.333 ± 0.13
1.041HisAsp: 1.041 ± 0.199
1.207HisGlu: 1.207 ± 0.221
0.874HisPhe: 0.874 ± 0.195
1.041HisGly: 1.041 ± 0.242
0.583HisHis: 0.583 ± 0.154
1.498HisIle: 1.498 ± 0.266
1.831HisLys: 1.831 ± 0.288
1.415HisLeu: 1.415 ± 0.226
0.666HisMet: 0.666 ± 0.192
0.999HisAsn: 0.999 ± 0.206
0.832HisPro: 0.832 ± 0.184
0.583HisGln: 0.583 ± 0.125
1.124HisArg: 1.124 ± 0.235
0.916HisSer: 0.916 ± 0.214
0.708HisThr: 0.708 ± 0.149
0.791HisVal: 0.791 ± 0.152
0.416HisTrp: 0.416 ± 0.137
0.832HisTyr: 0.832 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
4.786IleAla: 4.786 ± 0.476
0.624IleCys: 0.624 ± 0.177
5.036IleAsp: 5.036 ± 0.487
4.953IleGlu: 4.953 ± 0.408
1.831IlePhe: 1.831 ± 0.397
3.704IleGly: 3.704 ± 0.375
1.249IleHis: 1.249 ± 0.276
2.414IleIle: 2.414 ± 0.371
3.621IleLys: 3.621 ± 0.377
3.996IleLeu: 3.996 ± 0.411
1.249IleMet: 1.249 ± 0.282
3.33IleAsn: 3.33 ± 0.438
2.414IlePro: 2.414 ± 0.321
2.372IleGln: 2.372 ± 0.357
2.83IleArg: 2.83 ± 0.321
3.413IleSer: 3.413 ± 0.449
3.413IleThr: 3.413 ± 0.302
3.538IleVal: 3.538 ± 0.415
0.708IleTrp: 0.708 ± 0.163
1.706IleTyr: 1.706 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
5.452LysAla: 5.452 ± 0.606
0.666LysCys: 0.666 ± 0.187
4.287LysAsp: 4.287 ± 0.329
5.244LysGlu: 5.244 ± 0.438
1.915LysPhe: 1.915 ± 0.295
4.329LysGly: 4.329 ± 0.48
1.29LysHis: 1.29 ± 0.286
3.163LysIle: 3.163 ± 0.417
3.08LysLys: 3.08 ± 0.449
5.244LysLeu: 5.244 ± 0.586
2.747LysMet: 2.747 ± 0.401
3.08LysAsn: 3.08 ± 0.376
2.664LysPro: 2.664 ± 0.355
2.83LysGln: 2.83 ± 0.388
2.955LysArg: 2.955 ± 0.477
3.496LysSer: 3.496 ± 0.414
3.954LysThr: 3.954 ± 0.448
4.62LysVal: 4.62 ± 0.379
0.999LysTrp: 0.999 ± 0.194
2.789LysTyr: 2.789 ± 0.508
0.0LysXaa: 0.0 ± 0.0
Leu
6.535LeuAla: 6.535 ± 0.614
0.957LeuCys: 0.957 ± 0.224
6.368LeuAsp: 6.368 ± 0.473
5.869LeuGlu: 5.869 ± 0.586
2.83LeuPhe: 2.83 ± 0.292
4.995LeuGly: 4.995 ± 0.421
1.374LeuHis: 1.374 ± 0.203
5.328LeuIle: 5.328 ± 0.555
5.994LeuLys: 5.994 ± 0.53
6.368LeuLeu: 6.368 ± 0.582
2.414LeuMet: 2.414 ± 0.303
3.538LeuAsn: 3.538 ± 0.461
3.205LeuPro: 3.205 ± 0.406
3.33LeuGln: 3.33 ± 0.482
4.329LeuArg: 4.329 ± 0.367
4.995LeuSer: 4.995 ± 0.411
4.828LeuThr: 4.828 ± 0.322
5.494LeuVal: 5.494 ± 0.453
0.583LeuTrp: 0.583 ± 0.163
2.206LeuTyr: 2.206 ± 0.375
0.0LeuXaa: 0.0 ± 0.0
Met
2.664MetAla: 2.664 ± 0.41
0.291MetCys: 0.291 ± 0.138
1.79MetAsp: 1.79 ± 0.295
2.206MetGlu: 2.206 ± 0.343
0.874MetPhe: 0.874 ± 0.191
1.54MetGly: 1.54 ± 0.327
0.874MetHis: 0.874 ± 0.177
1.457MetIle: 1.457 ± 0.242
1.54MetLys: 1.54 ± 0.252
2.914MetLeu: 2.914 ± 0.338
0.874MetMet: 0.874 ± 0.187
2.081MetAsn: 2.081 ± 0.277
1.124MetPro: 1.124 ± 0.178
0.874MetGln: 0.874 ± 0.199
1.54MetArg: 1.54 ± 0.227
2.497MetSer: 2.497 ± 0.316
1.79MetThr: 1.79 ± 0.253
1.165MetVal: 1.165 ± 0.189
0.458MetTrp: 0.458 ± 0.126
1.415MetTyr: 1.415 ± 0.314
0.0MetXaa: 0.0 ± 0.0
Asn
4.287AsnAla: 4.287 ± 0.838
0.583AsnCys: 0.583 ± 0.193
2.997AsnAsp: 2.997 ± 0.388
2.289AsnGlu: 2.289 ± 0.342
2.289AsnPhe: 2.289 ± 0.291
3.788AsnGly: 3.788 ± 0.361
0.999AsnHis: 0.999 ± 0.173
2.83AsnIle: 2.83 ± 0.426
2.83AsnLys: 2.83 ± 0.343
4.287AsnLeu: 4.287 ± 0.329
1.207AsnMet: 1.207 ± 0.239
2.955AsnAsn: 2.955 ± 0.323
2.83AsnPro: 2.83 ± 0.416
2.622AsnGln: 2.622 ± 0.352
2.456AsnArg: 2.456 ± 0.298
3.746AsnSer: 3.746 ± 0.4
3.246AsnThr: 3.246 ± 0.426
3.538AsnVal: 3.538 ± 0.402
0.791AsnTrp: 0.791 ± 0.175
2.206AsnTyr: 2.206 ± 0.291
0.0AsnXaa: 0.0 ± 0.0
Pro
2.664ProAla: 2.664 ± 0.437
0.375ProCys: 0.375 ± 0.146
2.331ProAsp: 2.331 ± 0.349
3.246ProGlu: 3.246 ± 0.331
1.582ProPhe: 1.582 ± 0.314
1.706ProGly: 1.706 ± 0.31
0.499ProHis: 0.499 ± 0.114
2.164ProIle: 2.164 ± 0.349
2.039ProLys: 2.039 ± 0.322
2.705ProLeu: 2.705 ± 0.317
1.165ProMet: 1.165 ± 0.173
1.956ProAsn: 1.956 ± 0.237
1.124ProPro: 1.124 ± 0.285
1.706ProGln: 1.706 ± 0.25
1.29ProArg: 1.29 ± 0.247
2.331ProSer: 2.331 ± 0.319
2.83ProThr: 2.83 ± 0.355
2.747ProVal: 2.747 ± 0.308
0.458ProTrp: 0.458 ± 0.163
1.332ProTyr: 1.332 ± 0.247
0.0ProXaa: 0.0 ± 0.0
Gln
3.788GlnAla: 3.788 ± 0.622
0.208GlnCys: 0.208 ± 0.103
2.206GlnAsp: 2.206 ± 0.28
3.33GlnGlu: 3.33 ± 0.533
1.415GlnPhe: 1.415 ± 0.283
2.622GlnGly: 2.622 ± 0.411
0.541GlnHis: 0.541 ± 0.122
1.748GlnIle: 1.748 ± 0.272
2.747GlnLys: 2.747 ± 0.413
3.496GlnLeu: 3.496 ± 0.406
1.124GlnMet: 1.124 ± 0.294
1.665GlnAsn: 1.665 ± 0.206
1.29GlnPro: 1.29 ± 0.287
2.248GlnGln: 2.248 ± 0.33
1.998GlnArg: 1.998 ± 0.267
2.456GlnSer: 2.456 ± 0.268
2.331GlnThr: 2.331 ± 0.394
2.747GlnVal: 2.747 ± 0.389
0.416GlnTrp: 0.416 ± 0.122
1.582GlnTyr: 1.582 ± 0.294
0.0GlnXaa: 0.0 ± 0.0
Arg
3.08ArgAla: 3.08 ± 0.447
0.499ArgCys: 0.499 ± 0.158
3.579ArgAsp: 3.579 ± 0.387
3.788ArgGlu: 3.788 ± 0.456
1.748ArgPhe: 1.748 ± 0.239
2.83ArgGly: 2.83 ± 0.357
0.708ArgHis: 0.708 ± 0.197
2.289ArgIle: 2.289 ± 0.316
3.621ArgLys: 3.621 ± 0.474
4.662ArgLeu: 4.662 ± 0.478
1.415ArgMet: 1.415 ± 0.238
2.248ArgAsn: 2.248 ± 0.365
1.415ArgPro: 1.415 ± 0.269
1.748ArgGln: 1.748 ± 0.226
2.414ArgArg: 2.414 ± 0.27
2.497ArgSer: 2.497 ± 0.354
2.206ArgThr: 2.206 ± 0.264
2.997ArgVal: 2.997 ± 0.346
0.791ArgTrp: 0.791 ± 0.151
1.915ArgTyr: 1.915 ± 0.226
0.0ArgXaa: 0.0 ± 0.0
Ser
4.412SerAla: 4.412 ± 0.555
0.541SerCys: 0.541 ± 0.131
4.495SerAsp: 4.495 ± 0.585
4.412SerGlu: 4.412 ± 0.863
2.164SerPhe: 2.164 ± 0.263
4.578SerGly: 4.578 ± 0.42
1.124SerHis: 1.124 ± 0.246
3.371SerIle: 3.371 ± 0.368
4.662SerLys: 4.662 ± 0.407
5.078SerLeu: 5.078 ± 0.416
1.374SerMet: 1.374 ± 0.274
3.288SerAsn: 3.288 ± 0.405
2.747SerPro: 2.747 ± 0.306
1.374SerGln: 1.374 ± 0.244
2.414SerArg: 2.414 ± 0.291
2.872SerSer: 2.872 ± 0.377
3.663SerThr: 3.663 ± 0.368
3.704SerVal: 3.704 ± 0.402
0.957SerTrp: 0.957 ± 0.184
1.706SerTyr: 1.706 ± 0.266
0.0SerXaa: 0.0 ± 0.0
Thr
5.244ThrAla: 5.244 ± 0.514
0.541ThrCys: 0.541 ± 0.167
3.954ThrAsp: 3.954 ± 0.378
3.996ThrGlu: 3.996 ± 0.512
2.289ThrPhe: 2.289 ± 0.286
4.037ThrGly: 4.037 ± 0.447
1.457ThrHis: 1.457 ± 0.322
3.704ThrIle: 3.704 ± 0.409
4.37ThrLys: 4.37 ± 0.558
4.703ThrLeu: 4.703 ± 0.359
1.582ThrMet: 1.582 ± 0.227
3.038ThrAsn: 3.038 ± 0.525
2.872ThrPro: 2.872 ± 0.392
2.081ThrGln: 2.081 ± 0.222
2.372ThrArg: 2.372 ± 0.296
3.621ThrSer: 3.621 ± 0.474
3.746ThrThr: 3.746 ± 0.458
4.537ThrVal: 4.537 ± 0.462
0.749ThrTrp: 0.749 ± 0.171
2.747ThrTyr: 2.747 ± 0.379
0.0ThrXaa: 0.0 ± 0.0
Val
4.828ValAla: 4.828 ± 0.624
0.458ValCys: 0.458 ± 0.151
4.454ValAsp: 4.454 ± 0.403
5.036ValGlu: 5.036 ± 0.48
2.331ValPhe: 2.331 ± 0.288
4.578ValGly: 4.578 ± 0.366
1.082ValHis: 1.082 ± 0.223
3.746ValIle: 3.746 ± 0.323
4.662ValLys: 4.662 ± 0.392
4.454ValLeu: 4.454 ± 0.371
1.665ValMet: 1.665 ± 0.296
3.829ValAsn: 3.829 ± 0.403
1.915ValPro: 1.915 ± 0.265
2.497ValGln: 2.497 ± 0.329
3.413ValArg: 3.413 ± 0.395
3.788ValSer: 3.788 ± 0.435
4.204ValThr: 4.204 ± 0.368
4.287ValVal: 4.287 ± 0.423
0.791ValTrp: 0.791 ± 0.147
2.664ValTyr: 2.664 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
0.874TrpAla: 0.874 ± 0.198
0.125TrpCys: 0.125 ± 0.073
1.165TrpAsp: 1.165 ± 0.237
0.874TrpGlu: 0.874 ± 0.229
0.666TrpPhe: 0.666 ± 0.171
0.708TrpGly: 0.708 ± 0.128
0.583TrpHis: 0.583 ± 0.166
0.832TrpIle: 0.832 ± 0.207
0.832TrpLys: 0.832 ± 0.227
1.165TrpLeu: 1.165 ± 0.253
0.375TrpMet: 0.375 ± 0.119
0.916TrpAsn: 0.916 ± 0.267
0.208TrpPro: 0.208 ± 0.087
0.541TrpGln: 0.541 ± 0.146
0.458TrpArg: 0.458 ± 0.112
0.541TrpSer: 0.541 ± 0.151
0.957TrpThr: 0.957 ± 0.195
1.165TrpVal: 1.165 ± 0.23
0.0TrpTrp: 0.0 ± 0.0
0.624TrpTyr: 0.624 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.997TyrAla: 2.997 ± 0.314
0.458TyrCys: 0.458 ± 0.161
2.289TyrAsp: 2.289 ± 0.29
3.122TyrGlu: 3.122 ± 0.462
1.374TyrPhe: 1.374 ± 0.251
2.539TyrGly: 2.539 ± 0.349
0.832TyrHis: 0.832 ± 0.201
1.998TyrIle: 1.998 ± 0.322
1.915TyrLys: 1.915 ± 0.324
2.997TyrLeu: 2.997 ± 0.447
1.374TyrMet: 1.374 ± 0.247
1.998TyrAsn: 1.998 ± 0.262
1.29TyrPro: 1.29 ± 0.278
1.082TyrGln: 1.082 ± 0.192
2.289TyrArg: 2.289 ± 0.297
2.123TyrSer: 2.123 ± 0.439
2.123TyrThr: 2.123 ± 0.373
2.539TyrVal: 2.539 ± 0.324
0.708TyrTrp: 0.708 ± 0.159
1.124TyrTyr: 1.124 ± 0.27
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 114 proteins (24027 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski