Emmonsia crescens

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales;

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2B7Z9B6|A0A2B7Z9B6_9EURO Malonic semialdehyde reductase OS=Emmonsia crescens OX=73230 GN=GX50_07448 PE=3 SV=1
MM1 pKa = 7.46SPITMKK7 pKa = 10.19SWPLVTTLLILLFGLVASATQNNAGDD33 pKa = 4.27LVAITAVEE41 pKa = 3.95RR42 pKa = 11.84RR43 pKa = 11.84AEE45 pKa = 4.06GLEE48 pKa = 3.64VRR50 pKa = 11.84APKK53 pKa = 10.23GGKK56 pKa = 9.78GGGGGGGDD64 pKa = 5.33DD65 pKa = 6.07DD66 pKa = 7.09DD67 pKa = 7.65DD68 pKa = 6.66DD69 pKa = 7.17DD70 pKa = 5.9EE71 pKa = 7.35DD72 pKa = 5.22GSPTNQDD79 pKa = 2.89FQTCPNGGTTCTEE92 pKa = 4.19CFGAGYY98 pKa = 9.16VSCVTPSNICYY109 pKa = 10.27NPSTQTRR116 pKa = 11.84SQICTTQGGVSGSTQLAGSLGLGALGLAIALLL148 pKa = 4.08

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2B7Z9D0|A0A2B7Z9D0_9EURO MFS domain-containing protein OS=Emmonsia crescens OX=73230 GN=GX50_07408 PE=4 SV=1
MM1 pKa = 7.48SLFSAKK7 pKa = 10.17SLLEE11 pKa = 3.91SLPRR15 pKa = 11.84RR16 pKa = 11.84PTSSLSLFIKK26 pKa = 10.32SQSQSQSRR34 pKa = 11.84AFSHH38 pKa = 6.84LLSQRR43 pKa = 11.84RR44 pKa = 11.84LPTQQPTATSSAASSQPALLRR65 pKa = 11.84LLQPPTTATAQSTITTMTTGTLSSSVLSSPGATSRR100 pKa = 11.84PFSSSAALGAKK111 pKa = 9.84RR112 pKa = 11.84NTFNPSRR119 pKa = 11.84RR120 pKa = 11.84VQKK123 pKa = 10.2RR124 pKa = 11.84RR125 pKa = 11.84HH126 pKa = 5.34GFLARR131 pKa = 11.84LKK133 pKa = 8.2THH135 pKa = 6.44SGRR138 pKa = 11.84KK139 pKa = 7.82ILARR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84ARR147 pKa = 11.84GRR149 pKa = 11.84KK150 pKa = 7.83YY151 pKa = 10.92LSWW154 pKa = 5.23

Molecular weight: 16.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8899

0

8899

4332052

30

5954

486.8

53.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.016 ± 0.019

1.187 ± 0.01

5.655 ± 0.017

6.25 ± 0.023

3.648 ± 0.015

6.825 ± 0.024

2.455 ± 0.01

5.093 ± 0.019

5.016 ± 0.022

8.802 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.101 ± 0.009

3.98 ± 0.013

6.317 ± 0.031

4.076 ± 0.018

6.269 ± 0.022

8.646 ± 0.031

5.922 ± 0.017

5.748 ± 0.017

1.309 ± 0.008

2.684 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski