Lactobacillus phage Maenad

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tybeckvirinae; Maenadvirus; Lactobacillus virus Maenad

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P0ZKW3|A0A2P0ZKW3_9CAUD Uncharacterized protein OS=Lactobacillus phage Maenad OX=2079431 PE=4 SV=1
MM1 pKa = 7.82IEE3 pKa = 4.01LLLYY7 pKa = 10.42RR8 pKa = 11.84DD9 pKa = 3.73DD10 pKa = 3.67LKK12 pKa = 11.6YY13 pKa = 11.13YY14 pKa = 8.79ITEE17 pKa = 4.29EE18 pKa = 4.01YY19 pKa = 10.01TLQLDD24 pKa = 3.87RR25 pKa = 11.84DD26 pKa = 3.91WLVDD30 pKa = 3.59YY31 pKa = 10.64IKK33 pKa = 11.16NSTEE37 pKa = 3.86YY38 pKa = 11.12ASVDD42 pKa = 3.3EE43 pKa = 5.38FLEE46 pKa = 4.99FYY48 pKa = 10.81NSEE51 pKa = 4.01DD52 pKa = 3.56TEE54 pKa = 4.63EE55 pKa = 4.64IISTLDD61 pKa = 4.79DD62 pKa = 3.25MDD64 pKa = 3.95EE65 pKa = 4.64PYY67 pKa = 10.51TLEE70 pKa = 3.81KK71 pKa = 9.06TGRR74 pKa = 11.84YY75 pKa = 8.85CDD77 pKa = 4.75CDD79 pKa = 3.8DD80 pKa = 4.9LLL82 pKa = 6.19

Molecular weight: 9.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P0ZKQ0|A0A2P0ZKQ0_9CAUD Uncharacterized protein OS=Lactobacillus phage Maenad OX=2079431 PE=4 SV=1
MM1 pKa = 7.79LYY3 pKa = 10.61YY4 pKa = 10.44NHH6 pKa = 7.22RR7 pKa = 11.84KK8 pKa = 9.77KK9 pKa = 10.79KK10 pKa = 10.73GFVNMKK16 pKa = 9.72KK17 pKa = 10.09LVRR20 pKa = 11.84LLVVMVVSVIGVFTLNTINAKK41 pKa = 10.14AFSITGIKK49 pKa = 8.61LTRR52 pKa = 11.84TTNQINGGHH61 pKa = 7.28DD62 pKa = 2.8IFDD65 pKa = 3.62NVAITNTSRR74 pKa = 11.84HH75 pKa = 5.48RR76 pKa = 11.84ARR78 pKa = 11.84MVYY81 pKa = 10.1VVVKK85 pKa = 10.23EE86 pKa = 4.12KK87 pKa = 10.73GKK89 pKa = 10.61KK90 pKa = 9.62AIKK93 pKa = 9.68QNYY96 pKa = 8.15FIQPGGTVPVYY107 pKa = 7.56TTNKK111 pKa = 10.45DD112 pKa = 3.56EE113 pKa = 4.23VNSIYY118 pKa = 10.08RR119 pKa = 11.84RR120 pKa = 11.84KK121 pKa = 9.74FPRR124 pKa = 11.84KK125 pKa = 8.2FTVTVYY131 pKa = 10.77RR132 pKa = 11.84MSNKK136 pKa = 7.74QTMHH140 pKa = 6.59ALKK143 pKa = 10.42HH144 pKa = 4.63KK145 pKa = 8.32HH146 pKa = 4.52TWKK149 pKa = 10.15GMRR152 pKa = 11.84WTKK155 pKa = 10.76HH156 pKa = 4.5KK157 pKa = 9.94MAGGIEE163 pKa = 4.0

Molecular weight: 18.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

22225

23

1887

203.9

22.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.817 ± 0.495

0.553 ± 0.08

7.195 ± 0.447

6.178 ± 0.358

3.946 ± 0.202

6.974 ± 0.406

1.678 ± 0.119

6.407 ± 0.169

8.198 ± 0.401

8.234 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.148

5.926 ± 0.222

2.623 ± 0.157

3.514 ± 0.225

3.694 ± 0.206

6.947 ± 0.388

6.344 ± 0.33

6.641 ± 0.265

1.192 ± 0.094

4.409 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski