Amino acid dipepetide frequency for Lactobacillus phage Maenad

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.51AlaAla: 3.51 ± 0.9
0.27AlaCys: 0.27 ± 0.103
4.59AlaAsp: 4.59 ± 0.547
3.24AlaGlu: 3.24 ± 0.449
2.385AlaPhe: 2.385 ± 0.218
4.86AlaGly: 4.86 ± 0.744
0.675AlaHis: 0.675 ± 0.161
5.22AlaIle: 5.22 ± 0.573
5.76AlaLys: 5.76 ± 1.058
6.794AlaLeu: 6.794 ± 0.596
1.755AlaMet: 1.755 ± 0.316
3.375AlaAsn: 3.375 ± 0.481
1.665AlaPro: 1.665 ± 0.326
3.06AlaGln: 3.06 ± 0.457
2.475AlaArg: 2.475 ± 0.462
4.59AlaSer: 4.59 ± 0.891
4.5AlaThr: 4.5 ± 0.495
5.04AlaVal: 5.04 ± 0.637
0.99AlaTrp: 0.99 ± 0.235
3.42AlaTyr: 3.42 ± 0.439
0.0AlaXaa: 0.0 ± 0.0
Cys
0.27CysAla: 0.27 ± 0.104
0.0CysCys: 0.0 ± 0.0
0.54CysAsp: 0.54 ± 0.169
0.225CysGlu: 0.225 ± 0.096
0.18CysPhe: 0.18 ± 0.093
0.315CysGly: 0.315 ± 0.137
0.09CysHis: 0.09 ± 0.06
0.27CysIle: 0.27 ± 0.109
0.315CysLys: 0.315 ± 0.127
0.765CysLeu: 0.765 ± 0.157
0.135CysMet: 0.135 ± 0.087
0.225CysAsn: 0.225 ± 0.126
0.135CysPro: 0.135 ± 0.081
0.27CysGln: 0.27 ± 0.111
0.27CysArg: 0.27 ± 0.109
0.36CysSer: 0.36 ± 0.139
0.18CysThr: 0.18 ± 0.09
0.315CysVal: 0.315 ± 0.104
0.135CysTrp: 0.135 ± 0.075
0.54CysTyr: 0.54 ± 0.178
0.0CysXaa: 0.0 ± 0.0
Asp
3.06AspAla: 3.06 ± 0.318
0.54AspCys: 0.54 ± 0.141
6.659AspAsp: 6.659 ± 0.884
5.805AspGlu: 5.805 ± 0.69
3.195AspPhe: 3.195 ± 0.459
6.614AspGly: 6.614 ± 0.754
0.72AspHis: 0.72 ± 0.167
4.95AspIle: 4.95 ± 0.522
6.389AspLys: 6.389 ± 0.502
5.4AspLeu: 5.4 ± 0.698
1.935AspMet: 1.935 ± 0.322
3.825AspAsn: 3.825 ± 0.389
1.395AspPro: 1.395 ± 0.338
1.17AspGln: 1.17 ± 0.243
2.61AspArg: 2.61 ± 0.402
5.175AspSer: 5.175 ± 0.594
3.735AspThr: 3.735 ± 0.436
3.465AspVal: 3.465 ± 0.508
1.26AspTrp: 1.26 ± 0.258
4.05AspTyr: 4.05 ± 0.532
0.0AspXaa: 0.0 ± 0.0
Glu
4.725GluAla: 4.725 ± 0.529
0.27GluCys: 0.27 ± 0.128
4.59GluAsp: 4.59 ± 0.576
3.6GluGlu: 3.6 ± 0.435
2.385GluPhe: 2.385 ± 0.358
2.25GluGly: 2.25 ± 0.304
1.26GluHis: 1.26 ± 0.214
4.41GluIle: 4.41 ± 0.42
4.725GluLys: 4.725 ± 0.488
7.244GluLeu: 7.244 ± 0.752
2.61GluMet: 2.61 ± 0.355
2.835GluAsn: 2.835 ± 0.379
1.845GluPro: 1.845 ± 0.335
2.79GluGln: 2.79 ± 0.314
2.52GluArg: 2.52 ± 0.407
3.645GluSer: 3.645 ± 0.326
2.925GluThr: 2.925 ± 0.375
4.14GluVal: 4.14 ± 0.501
0.675GluTrp: 0.675 ± 0.153
2.34GluTyr: 2.34 ± 0.331
0.0GluXaa: 0.0 ± 0.0
Phe
2.475PheAla: 2.475 ± 0.311
0.405PheCys: 0.405 ± 0.127
3.465PheAsp: 3.465 ± 0.41
1.845PheGlu: 1.845 ± 0.336
1.935PhePhe: 1.935 ± 0.332
2.565PheGly: 2.565 ± 0.327
0.405PheHis: 0.405 ± 0.106
2.385PheIle: 2.385 ± 0.419
3.33PheLys: 3.33 ± 0.482
2.79PheLeu: 2.79 ± 0.47
1.08PheMet: 1.08 ± 0.217
2.61PheAsn: 2.61 ± 0.346
0.81PhePro: 0.81 ± 0.169
1.08PheGln: 1.08 ± 0.195
1.395PheArg: 1.395 ± 0.247
3.42PheSer: 3.42 ± 0.457
3.15PheThr: 3.15 ± 0.464
2.475PheVal: 2.475 ± 0.336
0.225PheTrp: 0.225 ± 0.098
1.62PheTyr: 1.62 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
4.68GlyAla: 4.68 ± 0.792
0.045GlyCys: 0.045 ± 0.047
4.725GlyAsp: 4.725 ± 0.673
3.69GlyGlu: 3.69 ± 0.423
3.24GlyPhe: 3.24 ± 0.439
4.86GlyGly: 4.86 ± 0.876
1.44GlyHis: 1.44 ± 0.212
4.59GlyIle: 4.59 ± 0.606
6.075GlyLys: 6.075 ± 1.083
6.389GlyLeu: 6.389 ± 0.701
1.395GlyMet: 1.395 ± 0.315
4.365GlyAsn: 4.365 ± 0.409
0.99GlyPro: 0.99 ± 0.262
2.115GlyGln: 2.115 ± 0.267
3.015GlyArg: 3.015 ± 0.42
4.635GlySer: 4.635 ± 0.608
4.455GlyThr: 4.455 ± 0.638
4.41GlyVal: 4.41 ± 0.392
1.125GlyTrp: 1.125 ± 0.192
3.51GlyTyr: 3.51 ± 0.315
0.0GlyXaa: 0.0 ± 0.0
His
0.945HisAla: 0.945 ± 0.158
0.09HisCys: 0.09 ± 0.061
1.35HisAsp: 1.35 ± 0.188
0.9HisGlu: 0.9 ± 0.221
0.945HisPhe: 0.945 ± 0.181
1.62HisGly: 1.62 ± 0.259
0.405HisHis: 0.405 ± 0.129
0.9HisIle: 0.9 ± 0.191
1.125HisLys: 1.125 ± 0.23
0.81HisLeu: 0.81 ± 0.167
0.36HisMet: 0.36 ± 0.126
1.125HisAsn: 1.125 ± 0.223
0.585HisPro: 0.585 ± 0.164
0.495HisGln: 0.495 ± 0.129
0.72HisArg: 0.72 ± 0.196
0.945HisSer: 0.945 ± 0.175
0.945HisThr: 0.945 ± 0.178
1.17HisVal: 1.17 ± 0.288
0.135HisTrp: 0.135 ± 0.079
1.215HisTyr: 1.215 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
5.04IleAla: 5.04 ± 0.423
0.45IleCys: 0.45 ± 0.125
4.635IleAsp: 4.635 ± 0.576
4.185IleGlu: 4.185 ± 0.49
2.43IlePhe: 2.43 ± 0.316
4.545IleGly: 4.545 ± 0.973
0.945IleHis: 0.945 ± 0.175
3.375IleIle: 3.375 ± 0.469
5.4IleLys: 5.4 ± 0.536
4.455IleLeu: 4.455 ± 0.49
1.53IleMet: 1.53 ± 0.303
5.31IleAsn: 5.31 ± 0.45
1.98IlePro: 1.98 ± 0.318
1.89IleGln: 1.89 ± 0.305
1.98IleArg: 1.98 ± 0.295
4.32IleSer: 4.32 ± 0.55
4.365IleThr: 4.365 ± 0.586
4.41IleVal: 4.41 ± 0.487
0.45IleTrp: 0.45 ± 0.122
2.385IleTyr: 2.385 ± 0.329
0.0IleXaa: 0.0 ± 0.0
Lys
5.805LysAla: 5.805 ± 0.864
0.27LysCys: 0.27 ± 0.122
4.995LysAsp: 4.995 ± 0.444
4.905LysGlu: 4.905 ± 0.536
2.925LysPhe: 2.925 ± 0.332
5.535LysGly: 5.535 ± 0.931
1.845LysHis: 1.845 ± 0.286
4.86LysIle: 4.86 ± 0.539
6.524LysLys: 6.524 ± 0.584
7.199LysLeu: 7.199 ± 0.642
3.195LysMet: 3.195 ± 0.451
4.14LysAsn: 4.14 ± 0.463
2.07LysPro: 2.07 ± 0.321
3.6LysGln: 3.6 ± 0.406
4.5LysArg: 4.5 ± 0.503
4.185LysSer: 4.185 ± 0.695
5.76LysThr: 5.76 ± 0.528
5.04LysVal: 5.04 ± 0.539
0.9LysTrp: 0.9 ± 0.34
3.735LysTyr: 3.735 ± 0.43
0.0LysXaa: 0.0 ± 0.0
Leu
5.715LeuAla: 5.715 ± 0.55
0.45LeuCys: 0.45 ± 0.157
6.254LeuAsp: 6.254 ± 0.705
6.254LeuGlu: 6.254 ± 0.566
2.925LeuPhe: 2.925 ± 0.349
5.445LeuGly: 5.445 ± 0.481
1.305LeuHis: 1.305 ± 0.272
5.04LeuIle: 5.04 ± 0.479
6.794LeuLys: 6.794 ± 0.539
6.704LeuLeu: 6.704 ± 0.575
2.43LeuMet: 2.43 ± 0.357
4.23LeuAsn: 4.23 ± 0.46
2.745LeuPro: 2.745 ± 0.313
2.25LeuGln: 2.25 ± 0.256
2.88LeuArg: 2.88 ± 0.402
6.479LeuSer: 6.479 ± 0.509
5.625LeuThr: 5.625 ± 0.482
4.86LeuVal: 4.86 ± 0.454
0.765LeuTrp: 0.765 ± 0.186
3.195LeuTyr: 3.195 ± 0.454
0.0LeuXaa: 0.0 ± 0.0
Met
2.025MetAla: 2.025 ± 0.287
0.0MetCys: 0.0 ± 0.0
1.215MetAsp: 1.215 ± 0.193
1.62MetGlu: 1.62 ± 0.284
0.675MetPhe: 0.675 ± 0.186
0.81MetGly: 0.81 ± 0.178
0.45MetHis: 0.45 ± 0.132
2.25MetIle: 2.25 ± 0.257
2.745MetLys: 2.745 ± 0.365
2.385MetLeu: 2.385 ± 0.355
0.45MetMet: 0.45 ± 0.13
1.755MetAsn: 1.755 ± 0.277
0.54MetPro: 0.54 ± 0.164
1.08MetGln: 1.08 ± 0.204
0.72MetArg: 0.72 ± 0.172
2.025MetSer: 2.025 ± 0.287
1.8MetThr: 1.8 ± 0.275
1.845MetVal: 1.845 ± 0.301
0.09MetTrp: 0.09 ± 0.061
0.81MetTyr: 0.81 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.78AsnAla: 3.78 ± 0.591
0.405AsnCys: 0.405 ± 0.136
4.14AsnAsp: 4.14 ± 0.414
3.69AsnGlu: 3.69 ± 0.362
1.8AsnPhe: 1.8 ± 0.281
5.76AsnGly: 5.76 ± 0.523
1.26AsnHis: 1.26 ± 0.228
3.33AsnIle: 3.33 ± 0.363
5.67AsnLys: 5.67 ± 0.501
3.825AsnLeu: 3.825 ± 0.462
1.44AsnMet: 1.44 ± 0.269
4.185AsnAsn: 4.185 ± 0.459
1.53AsnPro: 1.53 ± 0.243
2.565AsnGln: 2.565 ± 0.459
1.8AsnArg: 1.8 ± 0.291
3.465AsnSer: 3.465 ± 0.323
3.69AsnThr: 3.69 ± 0.405
3.87AsnVal: 3.87 ± 0.446
0.72AsnTrp: 0.72 ± 0.152
2.34AsnTyr: 2.34 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
2.115ProAla: 2.115 ± 0.327
0.225ProCys: 0.225 ± 0.115
2.25ProAsp: 2.25 ± 0.299
2.16ProGlu: 2.16 ± 0.42
0.72ProPhe: 0.72 ± 0.171
1.035ProGly: 1.035 ± 0.306
0.405ProHis: 0.405 ± 0.137
1.395ProIle: 1.395 ± 0.256
1.665ProLys: 1.665 ± 0.318
1.755ProLeu: 1.755 ± 0.275
0.45ProMet: 0.45 ± 0.14
1.71ProAsn: 1.71 ± 0.222
0.36ProPro: 0.36 ± 0.163
1.035ProGln: 1.035 ± 0.286
0.9ProArg: 0.9 ± 0.229
2.475ProSer: 2.475 ± 0.343
1.125ProThr: 1.125 ± 0.219
2.61ProVal: 2.61 ± 0.348
0.315ProTrp: 0.315 ± 0.109
1.53ProTyr: 1.53 ± 0.234
0.0ProXaa: 0.0 ± 0.0
Gln
2.385GlnAla: 2.385 ± 0.37
0.135GlnCys: 0.135 ± 0.087
1.62GlnAsp: 1.62 ± 0.237
1.71GlnGlu: 1.71 ± 0.266
1.755GlnPhe: 1.755 ± 0.29
2.16GlnGly: 2.16 ± 0.405
0.63GlnHis: 0.63 ± 0.143
2.43GlnIle: 2.43 ± 0.339
2.25GlnLys: 2.25 ± 0.313
3.06GlnLeu: 3.06 ± 0.385
0.81GlnMet: 0.81 ± 0.147
2.25GlnAsn: 2.25 ± 0.371
1.35GlnPro: 1.35 ± 0.396
1.35GlnGln: 1.35 ± 0.343
1.53GlnArg: 1.53 ± 0.254
2.385GlnSer: 2.385 ± 0.344
2.835GlnThr: 2.835 ± 0.504
2.205GlnVal: 2.205 ± 0.338
0.315GlnTrp: 0.315 ± 0.103
1.98GlnTyr: 1.98 ± 0.285
0.0GlnXaa: 0.0 ± 0.0
Arg
2.88ArgAla: 2.88 ± 0.363
0.18ArgCys: 0.18 ± 0.091
2.475ArgAsp: 2.475 ± 0.387
2.745ArgGlu: 2.745 ± 0.392
1.935ArgPhe: 1.935 ± 0.301
2.25ArgGly: 2.25 ± 0.324
0.855ArgHis: 0.855 ± 0.182
1.665ArgIle: 1.665 ± 0.288
2.97ArgLys: 2.97 ± 0.391
2.655ArgLeu: 2.655 ± 0.253
0.9ArgMet: 0.9 ± 0.225
2.115ArgAsn: 2.115 ± 0.334
0.99ArgPro: 0.99 ± 0.229
1.125ArgGln: 1.125 ± 0.241
1.35ArgArg: 1.35 ± 0.247
2.25ArgSer: 2.25 ± 0.363
2.52ArgThr: 2.52 ± 0.386
3.015ArgVal: 3.015 ± 0.416
0.585ArgTrp: 0.585 ± 0.172
1.485ArgTyr: 1.485 ± 0.258
0.0ArgXaa: 0.0 ± 0.0
Ser
5.67SerAla: 5.67 ± 0.93
0.225SerCys: 0.225 ± 0.092
5.4SerAsp: 5.4 ± 0.535
4.725SerGlu: 4.725 ± 0.553
2.52SerPhe: 2.52 ± 0.364
5.265SerGly: 5.265 ± 0.757
1.035SerHis: 1.035 ± 0.21
4.14SerIle: 4.14 ± 0.443
5.04SerLys: 5.04 ± 0.622
5.985SerLeu: 5.985 ± 0.507
1.26SerMet: 1.26 ± 0.252
4.14SerAsn: 4.14 ± 0.694
1.665SerPro: 1.665 ± 0.293
2.475SerGln: 2.475 ± 0.434
2.205SerArg: 2.205 ± 0.302
5.805SerSer: 5.805 ± 0.595
3.96SerThr: 3.96 ± 0.511
4.635SerVal: 4.635 ± 0.397
0.81SerTrp: 0.81 ± 0.172
2.52SerTyr: 2.52 ± 0.385
0.0SerXaa: 0.0 ± 0.0
Thr
4.95ThrAla: 4.95 ± 0.807
0.54ThrCys: 0.54 ± 0.182
4.005ThrAsp: 4.005 ± 0.41
3.375ThrGlu: 3.375 ± 0.386
2.43ThrPhe: 2.43 ± 0.332
5.04ThrGly: 5.04 ± 0.7
0.945ThrHis: 0.945 ± 0.213
4.815ThrIle: 4.815 ± 0.397
4.77ThrLys: 4.77 ± 0.423
4.815ThrLeu: 4.815 ± 0.471
1.215ThrMet: 1.215 ± 0.213
3.555ThrAsn: 3.555 ± 0.484
2.295ThrPro: 2.295 ± 0.333
2.475ThrGln: 2.475 ± 0.415
1.89ThrArg: 1.89 ± 0.316
4.14ThrSer: 4.14 ± 0.523
3.51ThrThr: 3.51 ± 0.403
4.95ThrVal: 4.95 ± 0.482
0.675ThrTrp: 0.675 ± 0.167
3.06ThrTyr: 3.06 ± 0.508
0.0ThrXaa: 0.0 ± 0.0
Val
4.815ValAla: 4.815 ± 0.525
0.405ValCys: 0.405 ± 0.152
4.725ValAsp: 4.725 ± 0.563
4.14ValGlu: 4.14 ± 0.506
2.205ValPhe: 2.205 ± 0.352
4.545ValGly: 4.545 ± 0.736
0.9ValHis: 0.9 ± 0.202
4.5ValIle: 4.5 ± 0.515
6.569ValLys: 6.569 ± 0.493
4.725ValLeu: 4.725 ± 0.545
1.215ValMet: 1.215 ± 0.237
4.23ValAsn: 4.23 ± 0.397
1.8ValPro: 1.8 ± 0.248
1.755ValGln: 1.755 ± 0.298
2.205ValArg: 2.205 ± 0.325
4.815ValSer: 4.815 ± 0.49
5.175ValThr: 5.175 ± 0.494
4.77ValVal: 4.77 ± 0.574
0.9ValTrp: 0.9 ± 0.197
2.025ValTyr: 2.025 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
0.585TrpAla: 0.585 ± 0.147
0.135TrpCys: 0.135 ± 0.075
0.81TrpAsp: 0.81 ± 0.201
0.585TrpGlu: 0.585 ± 0.145
0.945TrpPhe: 0.945 ± 0.238
0.765TrpGly: 0.765 ± 0.151
0.18TrpHis: 0.18 ± 0.09
0.81TrpIle: 0.81 ± 0.225
0.765TrpLys: 0.765 ± 0.221
1.395TrpLeu: 1.395 ± 0.249
0.09TrpMet: 0.09 ± 0.061
0.585TrpAsn: 0.585 ± 0.159
0.09TrpPro: 0.09 ± 0.066
0.54TrpGln: 0.54 ± 0.152
0.495TrpArg: 0.495 ± 0.119
1.035TrpSer: 1.035 ± 0.222
0.81TrpThr: 0.81 ± 0.177
0.54TrpVal: 0.54 ± 0.132
0.225TrpTrp: 0.225 ± 0.11
0.54TrpTyr: 0.54 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.745TyrAla: 2.745 ± 0.325
0.495TyrCys: 0.495 ± 0.163
3.51TyrAsp: 3.51 ± 0.544
2.385TyrGlu: 2.385 ± 0.371
1.935TyrPhe: 1.935 ± 0.355
3.51TyrGly: 3.51 ± 0.463
1.035TyrHis: 1.035 ± 0.264
2.745TyrIle: 2.745 ± 0.404
3.105TyrLys: 3.105 ± 0.359
3.195TyrLeu: 3.195 ± 0.413
0.99TyrMet: 0.99 ± 0.212
2.7TyrAsn: 2.7 ± 0.391
1.395TyrPro: 1.395 ± 0.247
2.025TyrGln: 2.025 ± 0.388
1.485TyrArg: 1.485 ± 0.258
3.33TyrSer: 3.33 ± 0.436
2.385TyrThr: 2.385 ± 0.459
2.655TyrVal: 2.655 ± 0.43
0.63TyrTrp: 0.63 ± 0.158
1.8TyrTyr: 1.8 ± 0.262
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (22225 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski