Helicobacter canadensis MIT 98-5491

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter canadensis

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1535 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C5ZV99|C5ZV99_9HELI Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta OS=Helicobacter canadensis MIT 98-5491 OX=537970 GN=accD PE=3 SV=1
MM1 pKa = 7.43AVKK4 pKa = 9.0ITDD7 pKa = 3.01ICIACGACIDD17 pKa = 4.08EE18 pKa = 5.06CPVEE22 pKa = 5.36AIVDD26 pKa = 4.06DD27 pKa = 5.38DD28 pKa = 5.63DD29 pKa = 4.85NPNNDD34 pKa = 2.27GCYY37 pKa = 9.23FVYY40 pKa = 10.69NNKK43 pKa = 9.64CVEE46 pKa = 4.47CVGHH50 pKa = 6.61NDD52 pKa = 3.28EE53 pKa = 4.91PACASACPTDD63 pKa = 4.4GCIVWDD69 pKa = 4.23AVVDD73 pKa = 4.25SQPHH77 pKa = 6.21RR78 pKa = 11.84DD79 pKa = 4.76DD80 pKa = 3.3IGEE83 pKa = 4.38DD84 pKa = 2.97KK85 pKa = 10.25RR86 pKa = 11.84TAHH89 pKa = 5.72VPVVEE94 pKa = 4.22

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C5ZXK1|C5ZXK1_9HELI Thiamine monophosphate kinase OS=Helicobacter canadensis MIT 98-5491 OX=537970 GN=thiL PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.31RR14 pKa = 11.84THH16 pKa = 5.85GFRR19 pKa = 11.84VRR21 pKa = 11.84MQTKK25 pKa = 9.97NGRR28 pKa = 11.84KK29 pKa = 9.25VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1535

0

1535

493512

31

2883

321.5

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.557 ± 0.058

1.197 ± 0.028

4.703 ± 0.042

7.184 ± 0.07

5.409 ± 0.066

6.102 ± 0.07

1.71 ± 0.024

8.762 ± 0.055

8.36 ± 0.062

10.736 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.026

5.641 ± 0.058

3.225 ± 0.036

3.98 ± 0.041

3.363 ± 0.04

6.56 ± 0.046

4.45 ± 0.047

5.3 ± 0.054

0.731 ± 0.02

3.775 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski