Duganella sp. Leaf126

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Duganella; unclassified Duganella

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4312 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q5CN11|A0A0Q5CN11_9BURK Uncharacterized protein OS=Duganella sp. Leaf126 OX=1736266 GN=ASF61_12385 PE=4 SV=1
MM1 pKa = 7.07NAVAEE6 pKa = 4.55SLDD9 pKa = 3.81VMPAPIVFTDD19 pKa = 3.8SAAEE23 pKa = 4.01KK24 pKa = 9.59VAQLIEE30 pKa = 4.38EE31 pKa = 4.51EE32 pKa = 4.63GNPDD36 pKa = 2.85LKK38 pKa = 11.13LRR40 pKa = 11.84VFVQGGGCSGFQYY53 pKa = 10.94GFTFDD58 pKa = 4.69EE59 pKa = 4.93IVNEE63 pKa = 4.83DD64 pKa = 3.85DD65 pKa = 3.11TTMVKK70 pKa = 10.56NGVEE74 pKa = 3.91LLIDD78 pKa = 3.72SMSYY82 pKa = 10.23QYY84 pKa = 11.55LVGAEE89 pKa = 4.01IDD91 pKa = 3.89YY92 pKa = 11.28KK93 pKa = 11.39DD94 pKa = 4.47DD95 pKa = 4.08LEE97 pKa = 4.56GAQFVIKK104 pKa = 10.68NPTATSTCGCGSSFSVV120 pKa = 3.54

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q5CZU2|A0A0Q5CZU2_9BURK Two-component system response regulator OS=Duganella sp. Leaf126 OX=1736266 GN=ASF61_08915 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84LAPLMRR8 pKa = 11.84LAPLMRR14 pKa = 11.84LAQLMRR20 pKa = 11.84LAPQMQRR27 pKa = 11.84TPLMRR32 pKa = 11.84LAPQMQRR39 pKa = 11.84TPLMRR44 pKa = 11.84LAPQMQRR51 pKa = 11.84TPLMPITLLTPLTT64 pKa = 3.98

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4312

0

4312

1538618

29

4569

356.8

38.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.432 ± 0.058

0.816 ± 0.012

5.65 ± 0.028

4.675 ± 0.034

3.369 ± 0.019

8.169 ± 0.042

2.215 ± 0.019

4.559 ± 0.03

3.347 ± 0.034

10.405 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.022

3.002 ± 0.026

5.001 ± 0.029

4.202 ± 0.028

6.487 ± 0.037

5.388 ± 0.034

5.536 ± 0.036

7.465 ± 0.031

1.298 ± 0.016

2.53 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski